Accelerated degradation of junctional acetylcholine receptor-α-bungarotoxin complexes in denervated rat diaphragm

R. S. Brett, S. G. Younkin, M. Konieczkowski, R. M. Slugg

Research output: Contribution to journalArticle

20 Scopus citations

Abstract

[125I]α-bungarotoxin was administered to rats in vivo to label acetylcholine receptors in innervated diaphragm, 5-day denervated diaphragm, or diaphragm which had been denervated immediately before labeling. The rate of degradation of junctional toxin-receptor complexes was followed by sacrificing animals at various times after labeling. The rate of degradation of junctional toxin-receptor complexes was significantly faster in 5-day denervated left hemidiaphragm (t 1 2 = 2.0 days) than in innervated left hemidiaphragm (t 1 2 = 10.7 days). The rate of degradation of junctional toxin-receptor complexes in left hemidiaphragm denervated at the time of labeling was essentially identical to that in innervated muscle for 3 days but then increased to a significantly more rapid rate (t 1 2 = 3.7 days in the period 3-13 days after denervation and labelling). These findings support the concert that continous innervation is needed to maintain the metabolic stability of junctional acetylcholine receptors.

Original languageEnglish (US)
Pages (from-to)133-142
Number of pages10
JournalBrain Research
Volume233
Issue number1
DOIs
StatePublished - Feb 4 1982

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Keywords

  • denervation
  • junctional acetylcholine receptors
  • turnover

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

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