A systematic approach to identifying urothelial cells likely to be polysomic by fluorescence in situ hybridization

Benjamin R. Kipp, Emily G. Barr Fritcher, Kristina M. Del Rosario, Carole L. Stevens, Thomas J. Sebo, Kevin C. Halling

Research output: Contribution to journalArticle

21 Scopus citations

Abstract

OBJECTIVE: To determine which nuclear morphologic and background features are most associated with normal and abnormal chromosome patterns in urine cells evaluated by fluorescence in situ hybridization (FISH) with Vysis® UroVysion (Downers Grove, Illinois, U.S.A.). STUDY DESIGN: One hundred specimens were analyzed and the nuclear morphologic and background features compared between FISH-negative (disomic) and -positive (polysomic) cases. RESULTS: Our data show that polysomic urothelial cells have significantly (p < 0.001) weaker DAPI nuclear counterstaining, nuclear enlargement, more irregular nuclear shape and more chromatin clumping when compared to disomic cells. CONCLUSION: These findings indicate that there are specific staining and nuclear cytologic features that can help identify cells that have a high probability of being polysomic by FISH. This information may help cytologists decrease the time required to evaluate urine specimens by FISH and may aid in the development of instruments that automate FISH analysis.

Original languageEnglish (US)
Pages (from-to)317-322
Number of pages6
JournalAnalytical and Quantitative Cytology and Histology
Volume27
Issue number6
StatePublished - Dec 1 2005

Keywords

  • Hybridization in situ, fluorescence
  • Morphometry
  • Urine
  • UroVysion
  • Urothelium

ASJC Scopus subject areas

  • Anatomy
  • Histology

Fingerprint Dive into the research topics of 'A systematic approach to identifying urothelial cells likely to be polysomic by fluorescence in situ hybridization'. Together they form a unique fingerprint.

  • Cite this

    Kipp, B. R., Barr Fritcher, E. G., Del Rosario, K. M., Stevens, C. L., Sebo, T. J., & Halling, K. C. (2005). A systematic approach to identifying urothelial cells likely to be polysomic by fluorescence in situ hybridization. Analytical and Quantitative Cytology and Histology, 27(6), 317-322.