A study of aminolevulinic acid-induced protoporphyrin IX fluorescence kinetics in the canine oral cavity

Vijay V. Vaidyanathan, Sohi Rastegar, Theresa W. Fossum, Patricia Flores, Egbertus W. Van Der Breggen, Norman G. Egger, Steven L. Jacques, Massoud Motamedi

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7 Scopus citations

Abstract

Background and Objective: 5-Aminolevulinic acid-induced protoporphyrin IX is a promising photosensitizer that could enhance the spectroscopic contrast between normal and diseased oral tissues. Knowledge of the pharmacokinetics and effects on tissue type are important for diagnostic and therapeutic procedures. Study Design/Materials and Methods: Dogs randomly were administered five doses of 5-aminolevulinic acid: 5, 25, 50, 75, and 100 mg/kg. The fluorescence was recorded from buccal mucosa, gums, tongue, and facial skin using a fiberoptic probe connected to an optical multichannel analyzer. Blood samples were collected for hematologic and serum biochemical analysis. Pharmacokinetic parameters of interest were estimated using a compartmental model. Results: Protoporphyrin fluorescence at all sites reached a peak in 2-6 hours, and returned to baseline in 24-31 hours, depending on the dose. Plasma protoporphyrin peaked earlier than oral tissues. Conclusion: The rate of synthesis of protoporphyrin, and its conversion to heme products are dose dependent. Different tissues have different pharmacokinetic response. (C) 2000 Wiley-Liss, Inc.

Original languageEnglish (US)
Pages (from-to)405-414
Number of pages10
JournalLasers in Surgery and Medicine
Volume26
Issue number4
DOIs
StatePublished - May 22 2000

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Keywords

  • Dosage
  • In vivo
  • Oral tissue
  • Pharmacokinetics

ASJC Scopus subject areas

  • Surgery
  • Dermatology

Cite this

Vaidyanathan, V. V., Rastegar, S., Fossum, T. W., Flores, P., Van Der Breggen, E. W., Egger, N. G., Jacques, S. L., & Motamedi, M. (2000). A study of aminolevulinic acid-induced protoporphyrin IX fluorescence kinetics in the canine oral cavity. Lasers in Surgery and Medicine, 26(4), 405-414. https://doi.org/10.1002/(SICI)1096-9101(2000)26:4<405::AID-LSM9>3.0.CO;2-E