TY - JOUR
T1 - A single-cell atlas of human and mouse white adipose tissue
AU - Emont, Margo P.
AU - Jacobs, Christopher
AU - Essene, Adam L.
AU - Pant, Deepti
AU - Tenen, Danielle
AU - Colleluori, Georgia
AU - Di Vincenzo, Angelica
AU - Jørgensen, Anja M.
AU - Dashti, Hesam
AU - Stefek, Adam
AU - McGonagle, Elizabeth
AU - Strobel, Sophie
AU - Laber, Samantha
AU - Agrawal, Saaket
AU - Westcott, Gregory P.
AU - Kar, Amrita
AU - Veregge, Molly L.
AU - Gulko, Anton
AU - Srinivasan, Harini
AU - Kramer, Zachary
AU - De Filippis, Eleanna
AU - Merkel, Erin
AU - Ducie, Jennifer
AU - Boyd, Christopher G.
AU - Gourash, William
AU - Courcoulas, Anita
AU - Lin, Samuel J.
AU - Lee, Bernard T.
AU - Morris, Donald
AU - Tobias, Adam
AU - Khera, Amit V.
AU - Claussnitzer, Melina
AU - Pers, Tune H.
AU - Giordano, Antonio
AU - Ashenberg, Orr
AU - Regev, Aviv
AU - Tsai, Linus T.
AU - Rosen, Evan D.
N1 - Funding Information:
S.A. has served as a scientific consultant to Third Rock Ventures. A.V.K. has served as a scientific advisor to Sanofi, Amgen, Maze Therapeutics, Navitor Pharmaceuticals, Sarepta Therapeutics, Novartis, Verve Therapeutics, Silence Therapeutics, Veritas International, Color Health, Third Rock Ventures and Columbia University (NIH); received speaking fees from Illumina, MedGenome, Amgen, and the Novartis Institute for Biomedical Research; and received a sponsored research agreement from the Novartis Institute for Biomedical Research. M.C. holds equity in Waypoint Bio and is a member of the Nestle Scientific Advisory Board. A.R. is a co-founder and equity holder of Celsius Therapeutics, an equity holder in Immunitas Therapeutics and a scientific advisory board member of Thermo Fisher Scientific, Syros Pharmaceuticals, Asimov and Neogene Therapeutics. A.R. is also an employee of Genentech. All other authors declare no competing interests.
Funding Information:
This work was supported by NIH grants RC2 DK116691 to E.D.R., L.T.T., A.C., O.A. and A.R., AHA POST14540015 and DoD PRMRP-DAW81XWH to L.T.T., Broad-BADERC Collaboration Initiative Award (NIH 5P30DK057521) to L.T.T. and E.D.R., and R01 DK102173 to E.D.R. M.P.E. is supported by NIH grant F32DK124914. Additional support includes PRIN 2017 (Italian Ministry of University, no. 2017L8Z2EM) to A. Giordano, T.H.P. acknowledges the Novo Nordisk Foundation (unconditional donation to the Novo Nordisk Foundation Center for Basic Metabolic Research; grant number NNF18CC0034900) and the Lundbeck Foundation (grant number R190-2014-3904), grants AMP-T2D RFB8b (FNIH) and UM1DK126185 (NIDDK) to M.C., Sarnoff Cardiovascular Research Foundation Fellowship to S.A., grants 1K08HG010155 and 1U01HG011719 to A.V.K. from the National Human Genome Research Institute, and a sponsored research agreement from IBM Research to the Broad Institute of MIT and Harvard to A.V.K. All single cell library construction and sequencing was performed through the Boston Nutrition Obesity Research Center Functional Genomics and Bioinformatics Core (NIH P30DK046200). We thank C. Usher for artistic support and M. Udler for helpful discussions.
Publisher Copyright:
© 2022, The Author(s), under exclusive licence to Springer Nature Limited.
PY - 2022/3/31
Y1 - 2022/3/31
N2 - White adipose tissue, once regarded as morphologically and functionally bland, is now recognized to be dynamic, plastic and heterogenous, and is involved in a wide array of biological processes including energy homeostasis, glucose and lipid handling, blood pressure control and host defence1. High-fat feeding and other metabolic stressors cause marked changes in adipose morphology, physiology and cellular composition1, and alterations in adiposity are associated with insulin resistance, dyslipidemia and type 2 diabetes2. Here we provide detailed cellular atlases of human and mouse subcutaneous and visceral white fat at single-cell resolution across a range of body weight. We identify subpopulations of adipocytes, adipose stem and progenitor cells, vascular and immune cells and demonstrate commonalities and differences across species and dietary conditions. We link specific cell types to increased risk of metabolic disease and provide an initial blueprint for a comprehensive set of interactions between individual cell types in the adipose niche in leanness and obesity. These data comprise an extensive resource for the exploration of genes, traits and cell types in the function of white adipose tissue across species, depots and nutritional conditions.
AB - White adipose tissue, once regarded as morphologically and functionally bland, is now recognized to be dynamic, plastic and heterogenous, and is involved in a wide array of biological processes including energy homeostasis, glucose and lipid handling, blood pressure control and host defence1. High-fat feeding and other metabolic stressors cause marked changes in adipose morphology, physiology and cellular composition1, and alterations in adiposity are associated with insulin resistance, dyslipidemia and type 2 diabetes2. Here we provide detailed cellular atlases of human and mouse subcutaneous and visceral white fat at single-cell resolution across a range of body weight. We identify subpopulations of adipocytes, adipose stem and progenitor cells, vascular and immune cells and demonstrate commonalities and differences across species and dietary conditions. We link specific cell types to increased risk of metabolic disease and provide an initial blueprint for a comprehensive set of interactions between individual cell types in the adipose niche in leanness and obesity. These data comprise an extensive resource for the exploration of genes, traits and cell types in the function of white adipose tissue across species, depots and nutritional conditions.
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U2 - 10.1038/s41586-022-04518-2
DO - 10.1038/s41586-022-04518-2
M3 - Article
C2 - 35296864
AN - SCOPUS:85126384772
SN - 0028-0836
VL - 603
SP - 926
EP - 933
JO - Nature
JF - Nature
IS - 7903
ER -