A novel source of viable peripheral blood mononuclear cells from leukoreduction system chambers

Allan B. Dietz, Peggy A. Bulur, Richard L. Emery, Jeffrey L. Winters, Dennis E. Epps, Abba C. Zubair, Stanimir Vuk-Pavlović

Research output: Contribution to journalArticle

77 Scopus citations

Abstract

BACKGROUND: Buffy coats are becoming less available as a source of research-grade peripheral blood mononuclear cells (PBMNCs). Therefore, alternative sources of these cells were investigated. STUDY DESIGN AND METHODS: PBMNCs isolated from the cells retained in leukoreduction system chambers (LRSCs) and those eluted from white blood cell filters were compared. From LRSCs (1.88 ± 0.40) × 109 PBMNCs (n = 13) versus (0.43 ± 0.15) × 109 PBMNCs were isolated from leukofilter eluates (LFEs, n = 8; p < 0.0001). RESULTS: Cells from LRSCs and LFEs produced similar numbers of burst-forming unit-erythroid, colony-forming unit (CFU)-granulocyte-macrophage, and CFU-granulocyte-erythrocyte-monocyte- macrophage-megakaryocyte colonies. The percentages of cells positive for CD3, CD4, CD8, CD14, CD19, and CD56 in the PBMNCs isolated from LRSCs and LFEs were indistinguishable. Cells isolated from LRSCs expressed higher levels of CD69 and CD25 in reaction to staphylococcal enterotoxin B than the cells isolated from LFEs. The source of cells affected neither the yield and purity of immunomagnetically isolated CD3+ cells, CD14+ cells, and CD56+ cells nor the function of T cells, natural killer cells, and in vitro matured dendritic cells (DCs). DC yield from LRSC-derived CD14+ cells, however, was higher. CONCLUSION: LRSCs are a novel source of fully functional PBMNCs that can replace the more traditional sources of research-grade cellular products.

Original languageEnglish (US)
Pages (from-to)2083-2089
Number of pages7
JournalTransfusion
Volume46
Issue number12
DOIs
StatePublished - Dec 1 2006

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ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Hematology

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