TY - JOUR
T1 - A novel Cryptosporidium parvum antigen, CP2, preferentially associates with membranous structures
AU - O'Hara, Steven P.
AU - Yu, Jae Ran
AU - Lin, Jim Jung Ching
N1 - Funding Information:
Acknowledgements We would like to thank Dr. George Cain for critical reading of the manuscript. This work was supported by grant HD18577 from the National Institutes of Health (J.J.C.L.) and grant 2000-1-20200-003-1 from the Basic Research Program of Korean Science and Engineering Foundation (J.R.Y.). The following reagents were obtained through the AIDS Research and Reference Reagent Program, Division of AIDS, NIAID, the National Institutes of Health: C. parvum oocysts provided by Ms. Marilyn Marshall and Dr. Charles Sterling, C. parvum cDNA and genomic libraries produced by BioTechnology General. The experiments comply with the current laws of the United States in which the experiments were performed. The nucleotide sequence reported in this paper has been submitted to the GenBank with accession number AY471868.
PY - 2004/3
Y1 - 2004/3
N2 - The present study addresses the cloning and characterization of a Cryptosporidium parvum antigen, CP2. Sequencing of cDNA and genomic clones revealed a novel gene capable of coding a message of 2,136 nucleotides flanked by 28 and 140 nucleotides of the 5′-and 3′-noncoding regions, respectively. The deduced amino acid sequence suggests that CP2 is a secreted and/ or membrane protein. Immunofluorescence microscopy detected CP2 enrichment in sporozoites that subsequently appeared to encase type I meronts in infected HCT-8 cells. Immunogold electron microscopy revealed that CP2 consistently localized to membranous structures throughout development. In addition, progression from macrogametocyte to sporulated oocyst revealed CP2 initially at the periphery of amylopectin-like granules, in the cytoplasm and discrete vesicles, the parasitophorous vacuole, on the surface of sporozoites, and finally on the parasitophorous vacuole membrane (PVM). The observed expression pattern suggests that CP2 may be involved in the invasion process and/or PVM integrity.
AB - The present study addresses the cloning and characterization of a Cryptosporidium parvum antigen, CP2. Sequencing of cDNA and genomic clones revealed a novel gene capable of coding a message of 2,136 nucleotides flanked by 28 and 140 nucleotides of the 5′-and 3′-noncoding regions, respectively. The deduced amino acid sequence suggests that CP2 is a secreted and/ or membrane protein. Immunofluorescence microscopy detected CP2 enrichment in sporozoites that subsequently appeared to encase type I meronts in infected HCT-8 cells. Immunogold electron microscopy revealed that CP2 consistently localized to membranous structures throughout development. In addition, progression from macrogametocyte to sporulated oocyst revealed CP2 initially at the periphery of amylopectin-like granules, in the cytoplasm and discrete vesicles, the parasitophorous vacuole, on the surface of sporozoites, and finally on the parasitophorous vacuole membrane (PVM). The observed expression pattern suggests that CP2 may be involved in the invasion process and/or PVM integrity.
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U2 - 10.1007/s00436-003-1057-5
DO - 10.1007/s00436-003-1057-5
M3 - Article
C2 - 14727189
AN - SCOPUS:1542614372
VL - 92
SP - 317
EP - 327
JO - Parasitology Research
JF - Parasitology Research
SN - 0932-0113
IS - 4
ER -