A novel Cryptosporidium parvum antigen, CP2, preferentially associates with membranous structures

Steven P. O'Hara, Jae R. Yu, J. J C Lin

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

The present study addresses the cloning and characterization of a Cryptosporidium parvum antigen, CP2. Sequencing of cDNA and genomic clones revealed a novel gene capable of coding a message of 2,136 nucleotides flanked by 28 and 140 nucleotides of the 5′-and 3′-noncoding regions, respectively. The deduced amino acid sequence suggests that CP2 is a secreted and/ or membrane protein. Immunofluorescence microscopy detected CP2 enrichment in sporozoites that subsequently appeared to encase type I meronts in infected HCT-8 cells. Immunogold electron microscopy revealed that CP2 consistently localized to membranous structures throughout development. In addition, progression from macrogametocyte to sporulated oocyst revealed CP2 initially at the periphery of amylopectin-like granules, in the cytoplasm and discrete vesicles, the parasitophorous vacuole, on the surface of sporozoites, and finally on the parasitophorous vacuole membrane (PVM). The observed expression pattern suggests that CP2 may be involved in the invasion process and/or PVM integrity.

Original languageEnglish (US)
Pages (from-to)317-327
Number of pages11
JournalParasitology Research
Volume92
Issue number4
DOIs
StatePublished - Mar 2004
Externally publishedYes

Fingerprint

Cryptosporidium parvum
Vacuoles
vacuoles
Sporozoites
sporozoites
antigens
Antigens
Nucleotides
nucleotides
meronts
Amylopectin
Membranes
Oocysts
amylopectin
fluorescence microscopy
Fluorescence Microscopy
oocysts
membrane proteins
Organism Cloning
granules

ASJC Scopus subject areas

  • Parasitology

Cite this

A novel Cryptosporidium parvum antigen, CP2, preferentially associates with membranous structures. / O'Hara, Steven P.; Yu, Jae R.; Lin, J. J C.

In: Parasitology Research, Vol. 92, No. 4, 03.2004, p. 317-327.

Research output: Contribution to journalArticle

O'Hara, Steven P. ; Yu, Jae R. ; Lin, J. J C. / A novel Cryptosporidium parvum antigen, CP2, preferentially associates with membranous structures. In: Parasitology Research. 2004 ; Vol. 92, No. 4. pp. 317-327.
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