A novel aptamer developed for breast cancer cell internalization

Kejing Zhang, Kwame Sefah, Lili Tang, Zilong Zhao, Guizhi Zhu, Mao Ye, Weijia Sun, Steve Goodison, Weihong Tan

Research output: Contribution to journalArticle

74 Scopus citations

Abstract

Breast cancer affects one in eight women in the United States, with a mortality rate that is second only to lung cancer. Although chemotherapy is widely used in breast cancer treatment, its side effects remain a challenge. One way to address this problem is through drug delivery by the internalization of cell-type-specific probes. Although nucleic acid aptamers are excellent probes for molecular recognition, only a few studies have demonstrated that aptamers can be internalized into living cells. Therefore, herein we report the development of a cancer-cell-specific DNA aptamer probe, KMF2-1a. By using the cell-SELEX method, this aptamer was selected against breast cancer cell line MCF-10AT1. Our results show that KMF2-1a is internalized efficiently and specifically to the endosome of target breast cancer cells. These results indicate that KMF2-1a is a promising agent for cell-type-specific intracellular delivery with both diagnostic and therapeutic implications.

Original languageEnglish (US)
Pages (from-to)79-84
Number of pages6
JournalChemMedChem
Volume7
Issue number1
DOIs
StatePublished - Jan 2 2012

    Fingerprint

Keywords

  • Aptamers
  • Breast cancer
  • Drug delivery
  • Internalization
  • MCF-10AT1

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Pharmacology
  • Drug Discovery
  • Pharmacology, Toxicology and Pharmaceutics(all)
  • Organic Chemistry

Cite this

Zhang, K., Sefah, K., Tang, L., Zhao, Z., Zhu, G., Ye, M., Sun, W., Goodison, S., & Tan, W. (2012). A novel aptamer developed for breast cancer cell internalization. ChemMedChem, 7(1), 79-84. https://doi.org/10.1002/cmdc.201100457