A multicompartmental model of in vivo adipose tissue glycerol kinetics and capillary permeability in lean and obese humans

Simon W. Coppack, David L. Chinkes, John M. Miles, Bruce W. Patterson, Samuel Klein

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Abstract

Lipolysis of adipose tissue triglycerides releases glycerol. Twenty-four volunteers, of whom 6 were obese and 13 were women, received a primed-constant infusion of 2H5-glycerol for 120 min during postabsorptive steady-state conditions. Arterial, abdominal venous, and interstitial (microdialysis) samples were taken, and a four-compartment model was applied to assess subcutaneous abdominal adipose tissue glycerol kinetics. Adipose tissue blood flow was measured using 133Xe washout. Venous glycerol concentrations (median 230 μmol/l [interquartile range 210-268]) were consistently greater than those of arterial blood (69.1 μmol/l [56.5-85.5]), while glycerol isotopic enrichments (tracer-to-tracee ratio) were greater in arterial blood (8.34% [7.44-10.1]) than venous blood (2.34% [1.71-2.69], P < 0.01). Microdialysate glycerol enrichment was 1.44% (1.11-1.79), indicating incomplete permeability of glycerol between capillary blood and interstitium. Calculated interstitial glycerol concentrations were between 270 μmol/l (256-350) and 332 μmol/l (281-371) (examining different boundary conditions). The calculated capillary diffusion capacity (ps) was between 2.21 ml · 100 g tissue-1 · min-1 (1.31-3.13) and 3.09 ml · 100 g tissue-1 · min-1 (1.52-4.90) and correlated inversely with adiposity (Rs ≤ -0.45, P < 0.05). Our results support previous estimates of interstitial glycerol concentration within adipose tissue and reveal capillary diffusion capacity is reduced in obesity.

Original languageEnglish (US)
Pages (from-to)1934-1941
Number of pages8
JournalDiabetes
Volume54
Issue number7
DOIs
StatePublished - Jul 2005

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Capillary Permeability
Glycerol
Adipose Tissue
Abdominal Subcutaneous Fat
Lipolysis
Microdialysis
Adiposity
Volunteers
Permeability
Triglycerides
Obesity

ASJC Scopus subject areas

  • Internal Medicine
  • Endocrinology, Diabetes and Metabolism

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A multicompartmental model of in vivo adipose tissue glycerol kinetics and capillary permeability in lean and obese humans. / Coppack, Simon W.; Chinkes, David L.; Miles, John M.; Patterson, Bruce W.; Klein, Samuel.

In: Diabetes, Vol. 54, No. 7, 07.2005, p. 1934-1941.

Research output: Contribution to journalArticle

Coppack, Simon W. ; Chinkes, David L. ; Miles, John M. ; Patterson, Bruce W. ; Klein, Samuel. / A multicompartmental model of in vivo adipose tissue glycerol kinetics and capillary permeability in lean and obese humans. In: Diabetes. 2005 ; Vol. 54, No. 7. pp. 1934-1941.
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