A microsystem integrating photodegradable hydrogel microstructures and reconfigurable microfluidics for single-cell analysis and retrieval

Kyung Jin Son, Dong Sik Shin, Timothy Kwa, Jungmok You, Yandong Gao, Alexander Revzin

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

We developed a micropatterned photodegradable hydrogel array integrated with reconfigurable microfluidics to enable cell secretion analysis and cell retrieval at the single-cell level. The activity of protease molecules secreted from single cells was monitored using FRET peptides entrapped inside microfabricated compartments. Antibody-modified gel islands tethering cells to the surface could be degraded by UV exposure to release specific single cells of interest.

Original languageEnglish (US)
Pages (from-to)637-641
Number of pages5
JournalLab on a Chip
Volume15
Issue number3
DOIs
StatePublished - Feb 7 2015
Externally publishedYes

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Single-Cell Analysis
Microfluidics
Hydrogel
Microsystems
Antibodies
Hydrogels
Peptides
Peptide Hydrolases
Gels
Microstructure
Molecules
Islands

ASJC Scopus subject areas

  • Bioengineering
  • Biochemistry
  • Chemistry(all)
  • Biomedical Engineering

Cite this

A microsystem integrating photodegradable hydrogel microstructures and reconfigurable microfluidics for single-cell analysis and retrieval. / Son, Kyung Jin; Shin, Dong Sik; Kwa, Timothy; You, Jungmok; Gao, Yandong; Revzin, Alexander.

In: Lab on a Chip, Vol. 15, No. 3, 07.02.2015, p. 637-641.

Research output: Contribution to journalArticle

Son, Kyung Jin ; Shin, Dong Sik ; Kwa, Timothy ; You, Jungmok ; Gao, Yandong ; Revzin, Alexander. / A microsystem integrating photodegradable hydrogel microstructures and reconfigurable microfluidics for single-cell analysis and retrieval. In: Lab on a Chip. 2015 ; Vol. 15, No. 3. pp. 637-641.
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