A Method for Multi-day Tracking of Gastrointestinal Smooth Muscle Contractile Patterns in Organotypic Culture

Peng Du, Amelia Mazzone, Stefan Calder, Anna Qian, Simon J. Gibbons, Gianrico Farrugia, Arthur Beyder

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Gastrointestinal (GI) motility is a key component of digestive health, and it is a complex both rhythmic and arrhythmic process governed by many physiological factors. The ability to accurately track the movements of the GI tissues in vitro over multiple days would provide valuable insights into GI tract physiology. A correlational analysis tracking algorithm was developed and applied to intestinal smooth muscle tissues that were maintained in organotypic culture. Physiologically relevant metrics, such as frequency, amplitude and motility index were independently assayed to quantify smooth muscle contractions. The results were validated by manually detected frequency determined using a standard software package. The algorithm was capable of tracking the changes in contractions of the same tissues over six days. This proof-of-concept study demonstrates the feasibility of long-term tracking of GI motility in organotypic cultures over multiple days. The approach allows study of the effects on GI smooth muscle contractility of direct controlled targeting of the cells and molecules in the GI tunica muscularis.

Original languageEnglish (US)
Title of host publication2019 41st Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2019
PublisherInstitute of Electrical and Electronics Engineers Inc.
Pages4791-4794
Number of pages4
ISBN (Electronic)9781538613115
DOIs
StatePublished - Jul 2019
Event41st Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2019 - Berlin, Germany
Duration: Jul 23 2019Jul 27 2019

Publication series

NameProceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS
ISSN (Print)1557-170X

Conference

Conference41st Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2019
CountryGermany
CityBerlin
Period7/23/197/27/19

ASJC Scopus subject areas

  • Signal Processing
  • Biomedical Engineering
  • Computer Vision and Pattern Recognition
  • Health Informatics

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  • Cite this

    Du, P., Mazzone, A., Calder, S., Qian, A., Gibbons, S. J., Farrugia, G., & Beyder, A. (2019). A Method for Multi-day Tracking of Gastrointestinal Smooth Muscle Contractile Patterns in Organotypic Culture. In 2019 41st Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2019 (pp. 4791-4794). [8857365] (Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS). Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/EMBC.2019.8857365