TY - JOUR
T1 - A high percentage of serum samples that test reactive by enzyme immunoassay for anti-brucella antibodies are not confirmed by the standard tube agglutination test
AU - Binnicker, Matthew J.
AU - Theel, Elitza S.
AU - Larsen, Staci M.
AU - Patel, Robin
PY - 2012/8
Y1 - 2012/8
N2 - We describe a retrospective analysis of Brucella enzyme immunoassay (EIA) IgM and IgG results compared to those of the standard tube agglutination test (SAT). Among 1,091 samples tested, 104 (9.5%) and 24 (2.2%) sera were positive by IgM and IgG EIA, respectively. Supplemental testing by SAT showed that 82.7% (86/104) of IgM EIA-reactive samples and 54.2% (13/24) of IgG EIA-reactive samples were negative by SAT. Testing all EIA screen-reactive samples by SAT is required when evaluating patients for potential brucellosis. Due to the limitations of serology, culture remains the gold standard for detecting Brucella infection.
AB - We describe a retrospective analysis of Brucella enzyme immunoassay (EIA) IgM and IgG results compared to those of the standard tube agglutination test (SAT). Among 1,091 samples tested, 104 (9.5%) and 24 (2.2%) sera were positive by IgM and IgG EIA, respectively. Supplemental testing by SAT showed that 82.7% (86/104) of IgM EIA-reactive samples and 54.2% (13/24) of IgG EIA-reactive samples were negative by SAT. Testing all EIA screen-reactive samples by SAT is required when evaluating patients for potential brucellosis. Due to the limitations of serology, culture remains the gold standard for detecting Brucella infection.
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U2 - 10.1128/CVI.00197-12
DO - 10.1128/CVI.00197-12
M3 - Article
C2 - 22718127
AN - SCOPUS:84864567795
SN - 1556-6811
VL - 19
SP - 1332
EP - 1334
JO - Clinical and Vaccine Immunology
JF - Clinical and Vaccine Immunology
IS - 8
ER -