A high percentage of serum samples that test reactive by enzyme immunoassay for anti-brucella antibodies are not confirmed by the standard tube agglutination test

Matthew J. Binnicker, Elitza S. Theel, Staci M. Larsen, Robin Patel

Research output: Contribution to journalArticle

7 Scopus citations


We describe a retrospective analysis of Brucella enzyme immunoassay (EIA) IgM and IgG results compared to those of the standard tube agglutination test (SAT). Among 1,091 samples tested, 104 (9.5%) and 24 (2.2%) sera were positive by IgM and IgG EIA, respectively. Supplemental testing by SAT showed that 82.7% (86/104) of IgM EIA-reactive samples and 54.2% (13/24) of IgG EIA-reactive samples were negative by SAT. Testing all EIA screen-reactive samples by SAT is required when evaluating patients for potential brucellosis. Due to the limitations of serology, culture remains the gold standard for detecting Brucella infection.

Original languageEnglish (US)
Pages (from-to)1332-1334
Number of pages3
JournalClinical and Vaccine Immunology
Issue number8
StatePublished - Aug 1 2012


ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Clinical Biochemistry
  • Microbiology (medical)

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