A factor found in the igg fraction of serum of patients with paraneoplastic bilateral diffuse uveal melanocytic proliferation causes proliferation of cultured human melanocytes

Sarah L. Miles, Richard M. Niles, Sean J Pittock, Richard Geoffrey Vile, John Davies, Jeffrey L. Winters, Nakhleh E. Abu-Yaghi, Axel F Grothey, Mustaqeem Siddiqui, Judith S Kaur, Lynn Hartmann, Kimberly R. Kalli, Larry R Pease, Daniel Kravitz, Svetomir Nenad Markovic, Jose S Pulido

Research output: Contribution to journalArticle

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Abstract

PURPOSE: To determine if there is a factor in the serum of patients with bilateral diffuse uveal melanocytic proliferation (BDUMP) that causes melanocytic proliferation. METHODS: Human melanocytes and melanoma cells were grown and exposed to serum or plasma of patients with BDUMP, other neoplastic conditions, or control media. Preliminary studies using serum were conducted in an unmasked fashion. In addition, IgG-depleted and IgG-enriched plasma was also tested in a similar fashion. Experiments using plasma were conducted triple masked. To show that the proliferation was melanocyte selective, human dermal fibroblasts, keratinocytes, and ovarian cancer cells were treated with plasma of the BDUMP cases or controls, and the effect of this exposure on their proliferation was quantified. RESULTS: At 72 hours, the serum of BDUMP patients caused statistically significant increased proliferation of normal human melanocytes. Further studies at 6 days demonstrated similar findings. In addition, melanocytes grown in BDUMP serum exhibited a disorganized morphology with foci of multilayered cells. Cultured melanoma cells also showed statistically significant increase in growth in serum from BDUMP patients compared with controls. Masked plasma studies further confirmed these findings and showed that the IgG fraction appeared to contain the melanocyte growth-stimulating factor. The human fibroblasts, keratinocytes, and ovarian cancer cells did not show an increase in growth with the BDUMP plasma treatment. CONCLUSION: Patients with BDUMP have a factor in the IgG fraction that selectively causes melanocyte proliferation. How it causes proliferation of human melanocytes and melanoma cells needs to be further elucidated.

Original languageEnglish (US)
Pages (from-to)1959-1966
Number of pages8
JournalRetina
Volume32
Issue number9
DOIs
StatePublished - Oct 2012

Fingerprint

Ocular Paraneoplastic Syndromes
Melanocytes
Serum
Immunoglobulin G
Melanoma
Keratinocytes
Ovarian Neoplasms
Fibroblasts
Growth
Cultured Cells
Intercellular Signaling Peptides and Proteins
Skin

Keywords

  • BDUMP
  • bilateral diffuse uveal melanocytic proliferation
  • cancer
  • immunoglobulins
  • melanocytes
  • ovarian cancer
  • paraneoplastic syndromes
  • uveal melanocytic proliferation

ASJC Scopus subject areas

  • Ophthalmology

Cite this

A factor found in the igg fraction of serum of patients with paraneoplastic bilateral diffuse uveal melanocytic proliferation causes proliferation of cultured human melanocytes. / Miles, Sarah L.; Niles, Richard M.; Pittock, Sean J; Vile, Richard Geoffrey; Davies, John; Winters, Jeffrey L.; Abu-Yaghi, Nakhleh E.; Grothey, Axel F; Siddiqui, Mustaqeem; Kaur, Judith S; Hartmann, Lynn; Kalli, Kimberly R.; Pease, Larry R; Kravitz, Daniel; Markovic, Svetomir Nenad; Pulido, Jose S.

In: Retina, Vol. 32, No. 9, 10.2012, p. 1959-1966.

Research output: Contribution to journalArticle

Miles, Sarah L. ; Niles, Richard M. ; Pittock, Sean J ; Vile, Richard Geoffrey ; Davies, John ; Winters, Jeffrey L. ; Abu-Yaghi, Nakhleh E. ; Grothey, Axel F ; Siddiqui, Mustaqeem ; Kaur, Judith S ; Hartmann, Lynn ; Kalli, Kimberly R. ; Pease, Larry R ; Kravitz, Daniel ; Markovic, Svetomir Nenad ; Pulido, Jose S. / A factor found in the igg fraction of serum of patients with paraneoplastic bilateral diffuse uveal melanocytic proliferation causes proliferation of cultured human melanocytes. In: Retina. 2012 ; Vol. 32, No. 9. pp. 1959-1966.
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abstract = "PURPOSE: To determine if there is a factor in the serum of patients with bilateral diffuse uveal melanocytic proliferation (BDUMP) that causes melanocytic proliferation. METHODS: Human melanocytes and melanoma cells were grown and exposed to serum or plasma of patients with BDUMP, other neoplastic conditions, or control media. Preliminary studies using serum were conducted in an unmasked fashion. In addition, IgG-depleted and IgG-enriched plasma was also tested in a similar fashion. Experiments using plasma were conducted triple masked. To show that the proliferation was melanocyte selective, human dermal fibroblasts, keratinocytes, and ovarian cancer cells were treated with plasma of the BDUMP cases or controls, and the effect of this exposure on their proliferation was quantified. RESULTS: At 72 hours, the serum of BDUMP patients caused statistically significant increased proliferation of normal human melanocytes. Further studies at 6 days demonstrated similar findings. In addition, melanocytes grown in BDUMP serum exhibited a disorganized morphology with foci of multilayered cells. Cultured melanoma cells also showed statistically significant increase in growth in serum from BDUMP patients compared with controls. Masked plasma studies further confirmed these findings and showed that the IgG fraction appeared to contain the melanocyte growth-stimulating factor. The human fibroblasts, keratinocytes, and ovarian cancer cells did not show an increase in growth with the BDUMP plasma treatment. CONCLUSION: Patients with BDUMP have a factor in the IgG fraction that selectively causes melanocyte proliferation. How it causes proliferation of human melanocytes and melanoma cells needs to be further elucidated.",
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T1 - A factor found in the igg fraction of serum of patients with paraneoplastic bilateral diffuse uveal melanocytic proliferation causes proliferation of cultured human melanocytes

AU - Miles, Sarah L.

AU - Niles, Richard M.

AU - Pittock, Sean J

AU - Vile, Richard Geoffrey

AU - Davies, John

AU - Winters, Jeffrey L.

AU - Abu-Yaghi, Nakhleh E.

AU - Grothey, Axel F

AU - Siddiqui, Mustaqeem

AU - Kaur, Judith S

AU - Hartmann, Lynn

AU - Kalli, Kimberly R.

AU - Pease, Larry R

AU - Kravitz, Daniel

AU - Markovic, Svetomir Nenad

AU - Pulido, Jose S

PY - 2012/10

Y1 - 2012/10

N2 - PURPOSE: To determine if there is a factor in the serum of patients with bilateral diffuse uveal melanocytic proliferation (BDUMP) that causes melanocytic proliferation. METHODS: Human melanocytes and melanoma cells were grown and exposed to serum or plasma of patients with BDUMP, other neoplastic conditions, or control media. Preliminary studies using serum were conducted in an unmasked fashion. In addition, IgG-depleted and IgG-enriched plasma was also tested in a similar fashion. Experiments using plasma were conducted triple masked. To show that the proliferation was melanocyte selective, human dermal fibroblasts, keratinocytes, and ovarian cancer cells were treated with plasma of the BDUMP cases or controls, and the effect of this exposure on their proliferation was quantified. RESULTS: At 72 hours, the serum of BDUMP patients caused statistically significant increased proliferation of normal human melanocytes. Further studies at 6 days demonstrated similar findings. In addition, melanocytes grown in BDUMP serum exhibited a disorganized morphology with foci of multilayered cells. Cultured melanoma cells also showed statistically significant increase in growth in serum from BDUMP patients compared with controls. Masked plasma studies further confirmed these findings and showed that the IgG fraction appeared to contain the melanocyte growth-stimulating factor. The human fibroblasts, keratinocytes, and ovarian cancer cells did not show an increase in growth with the BDUMP plasma treatment. CONCLUSION: Patients with BDUMP have a factor in the IgG fraction that selectively causes melanocyte proliferation. How it causes proliferation of human melanocytes and melanoma cells needs to be further elucidated.

AB - PURPOSE: To determine if there is a factor in the serum of patients with bilateral diffuse uveal melanocytic proliferation (BDUMP) that causes melanocytic proliferation. METHODS: Human melanocytes and melanoma cells were grown and exposed to serum or plasma of patients with BDUMP, other neoplastic conditions, or control media. Preliminary studies using serum were conducted in an unmasked fashion. In addition, IgG-depleted and IgG-enriched plasma was also tested in a similar fashion. Experiments using plasma were conducted triple masked. To show that the proliferation was melanocyte selective, human dermal fibroblasts, keratinocytes, and ovarian cancer cells were treated with plasma of the BDUMP cases or controls, and the effect of this exposure on their proliferation was quantified. RESULTS: At 72 hours, the serum of BDUMP patients caused statistically significant increased proliferation of normal human melanocytes. Further studies at 6 days demonstrated similar findings. In addition, melanocytes grown in BDUMP serum exhibited a disorganized morphology with foci of multilayered cells. Cultured melanoma cells also showed statistically significant increase in growth in serum from BDUMP patients compared with controls. Masked plasma studies further confirmed these findings and showed that the IgG fraction appeared to contain the melanocyte growth-stimulating factor. The human fibroblasts, keratinocytes, and ovarian cancer cells did not show an increase in growth with the BDUMP plasma treatment. CONCLUSION: Patients with BDUMP have a factor in the IgG fraction that selectively causes melanocyte proliferation. How it causes proliferation of human melanocytes and melanoma cells needs to be further elucidated.

KW - BDUMP

KW - bilateral diffuse uveal melanocytic proliferation

KW - cancer

KW - immunoglobulins

KW - melanocytes

KW - ovarian cancer

KW - paraneoplastic syndromes

KW - uveal melanocytic proliferation

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U2 - 10.1097/IAE.0b013e3182618bab

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AN - SCOPUS:84866896086

VL - 32

SP - 1959

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JO - Retina

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SN - 0275-004X

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