A dynamin-cortactin-Arp2/3 complex mediates actin reorganization in growth factor-stimulated cells

Eugene W. Krueger, James D. Orth, Hong Cao, Mark A. McNiven

Research output: Contribution to journalArticlepeer-review

172 Scopus citations

Abstract

The mechanisms by which mammalian cells remodel the actin cytoskeleton in response to motogenic stimuli are complex and a topic of intense study. Dynamin 2 (Dyn2) is a large GTPase that interacts directly with several actin binding proteins, including cortactin. In this study, we demonstrate that Dyn2 and cortactin function to mediate dynamic remodeling of the actin cytoskeleton in response to stimulation with the motogenic growth factor platelet-derived growth factor. On stimulation, Dyn2 and cortactin coassemble into large, circular structures on the dorsal cell surface. These "waves" promote an active reorganization of actin filaments in the anterior cytoplasm and function to disassemble actin stress fibers. Importantly, inhibition of Dyn2 and cortactin function potently blocked the formation of waves and subsequent actin reorganization. These findings demonstrate that cortactin and Dyn2 function together in a supramolecular complex that assembles in response to growth factor stimulation and mediates the remodeling of actin to facilitate lamellipodial protrusion at the leading edge of migrating cells.

Original languageEnglish (US)
Pages (from-to)1085-1096
Number of pages12
JournalMolecular biology of the cell
Volume14
Issue number3
DOIs
StatePublished - Mar 1 2003

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Fingerprint Dive into the research topics of 'A dynamin-cortactin-Arp2/3 complex mediates actin reorganization in growth factor-stimulated cells'. Together they form a unique fingerprint.

Cite this