A Disposable Tear Glucose Biosensor—Part 5: Improvements in Reagents and Tear Sampling Component

Chi En Lin, Yuka Ito, Anna Deng, Jared Johns, Daniel Matloff, Curtiss B. Cook, Koji Sode, Jeffrey T. La Belle

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

A tear glucose (TG) sensor with an integrated tear sampler can provide a noninvasive method for calibrating the continuous TG contact lens and monitoring glucose. Expanding from previous work, an improved TG sensor that implements dried reagents, genetically modified glucose dehydrogenase (GDH), and a tear sampler was developed and compared against the TG sensor prepared with commercial GDH. It was found that neither sensor was affected by the tear interferents: ascorbic acid, acetaminophen, and uric acid. The sensor prepared with commercial GDH generated higher current. This suggests that using enzymes with lower K m may be advantageous when operating in low glucose environments like tears. The improved TG sensor also demonstrated the potential of integrating Schirmer’s test strip as a tear sampler for self-monitoring of TG.

Original languageEnglish (US)
Pages (from-to)842-846
Number of pages5
JournalJournal of Diabetes Science and Technology
Volume12
Issue number4
DOIs
StatePublished - Jul 1 2018

Keywords

  • Schirmer’s test strip
  • diabetes mellitus
  • glucose dehydrogenase
  • integrated tear glucose sensor
  • noninvasive glucose monitoring

ASJC Scopus subject areas

  • Internal Medicine
  • Endocrinology, Diabetes and Metabolism
  • Bioengineering
  • Biomedical Engineering

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    Lin, C. E., Ito, Y., Deng, A., Johns, J., Matloff, D., Cook, C. B., Sode, K., & La Belle, J. T. (2018). A Disposable Tear Glucose Biosensor—Part 5: Improvements in Reagents and Tear Sampling Component. Journal of Diabetes Science and Technology, 12(4), 842-846. https://doi.org/10.1177/1932296818769944