TY - JOUR
T1 - A Disposable Tear Glucose Biosensor—Part 5
T2 - Improvements in Reagents and Tear Sampling Component
AU - Lin, Chi En
AU - Ito, Yuka
AU - Deng, Anna
AU - Johns, Jared
AU - Matloff, Daniel
AU - Cook, Curtiss B.
AU - Sode, Koji
AU - La Belle, Jeffrey T.
N1 - Publisher Copyright:
© 2018, 2018 Diabetes Technology Society.
PY - 2018/7/1
Y1 - 2018/7/1
N2 - A tear glucose (TG) sensor with an integrated tear sampler can provide a noninvasive method for calibrating the continuous TG contact lens and monitoring glucose. Expanding from previous work, an improved TG sensor that implements dried reagents, genetically modified glucose dehydrogenase (GDH), and a tear sampler was developed and compared against the TG sensor prepared with commercial GDH. It was found that neither sensor was affected by the tear interferents: ascorbic acid, acetaminophen, and uric acid. The sensor prepared with commercial GDH generated higher current. This suggests that using enzymes with lower K m may be advantageous when operating in low glucose environments like tears. The improved TG sensor also demonstrated the potential of integrating Schirmer’s test strip as a tear sampler for self-monitoring of TG.
AB - A tear glucose (TG) sensor with an integrated tear sampler can provide a noninvasive method for calibrating the continuous TG contact lens and monitoring glucose. Expanding from previous work, an improved TG sensor that implements dried reagents, genetically modified glucose dehydrogenase (GDH), and a tear sampler was developed and compared against the TG sensor prepared with commercial GDH. It was found that neither sensor was affected by the tear interferents: ascorbic acid, acetaminophen, and uric acid. The sensor prepared with commercial GDH generated higher current. This suggests that using enzymes with lower K m may be advantageous when operating in low glucose environments like tears. The improved TG sensor also demonstrated the potential of integrating Schirmer’s test strip as a tear sampler for self-monitoring of TG.
KW - Schirmer’s test strip
KW - diabetes mellitus
KW - glucose dehydrogenase
KW - integrated tear glucose sensor
KW - noninvasive glucose monitoring
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U2 - 10.1177/1932296818769944
DO - 10.1177/1932296818769944
M3 - Article
AN - SCOPUS:85045829931
SN - 1932-2968
VL - 12
SP - 842
EP - 846
JO - Journal of Diabetes Science and Technology
JF - Journal of Diabetes Science and Technology
IS - 4
ER -