A critical role for vesicle-associated membrane protein-7 in exocytosis from human eosinophils and neutrophils

M. R. Logan, P. Lacy, S. O. Odemuyiwa, M. Steward, F. Davoine, Hirohito Kita, R. Moqbel

Research output: Contribution to journalArticle

60 Citations (Scopus)

Abstract

Background: Granulocyte exocytosis is proposed to be critically dependent on the interaction of soluble N-ethylmaleimide-sensitive factor attachment protein (SNAP) receptors (SNAREs) located on granules/vesicles (v-SNAREs) and plasma membrane (t-SNAREs). Previous studies indicated that the v-SNARE, vesicle-associated membrane protein (VAMP)-2, as well as t-SNAREs (SNAP-23, syntaxin-4 and -6) are implicated in exocytosis from human granulocytes. Vesicle-associated membrane proteins-7 and -8 have been implicated in endosome/lysosome trafficking, however, their role in granulocyte exocytosis remains obscure. Objective: We sought to investigate the expression and functional role of SNARE isoforms in the secretion of different granule-derived mediators in human eosinophils and neutrophils. Methods: The expression of SNAREs was determined by subcellular fractionation and flow cytometry. SNARE-specific antibodies were examined for their ability to impair mediator release from permeabilized eosinophils and neutrophils. Results: Vesicle-associated membrane proteins-7 and -8 were localized to granule and membrane-enriched fractions in eosinophils and neutrophils, whereas syntaxin-6 was not detectable. In permeabilized cells, anti-VAMP-7, but not anti-VAMP-8, antibody impaired the secretion of all mediators examined (in eosinophils, eosinophil peroxidase and eosinophil-derived neurotoxin; in neutrophils, myeloperoxidase, lactoferrin and matrix metalloprotease-9) in a dose-dependent manner. In contrast, anti-VAMP-2 modestly and selectively impaired secretion from small granules and vesicles. Syntaxin-4, but not syntaxin-6, was found to interact with SNAP-23 and was partially involved in mediator secretion from multiple compartments. Conclusion: Our observations indicate for the first time a critical role for VAMP-7 in both eosinophil and neutrophil mediator release.

Original languageEnglish (US)
Pages (from-to)777-784
Number of pages8
JournalAllergy: European Journal of Allergy and Clinical Immunology
Volume61
Issue number6
DOIs
StatePublished - Jun 2006

Fingerprint

R-SNARE Proteins
SNARE Proteins
Exocytosis
Eosinophils
Neutrophils
Qa-SNARE Proteins
Vesicle-Associated Membrane Protein 2
Granulocytes
Eosinophil-Derived Neurotoxin
Eosinophil Peroxidase
Lactoferrin
Antibodies
Endosomes
Metalloproteases
Lysosomes
Peroxidase
Flow Cytometry
Protein Isoforms
Proteins
Cell Membrane

Keywords

  • Exocytosis
  • Granulocytes
  • Soluble N-ethylmaleimide-sensitive factor attachment protein receptors
  • Vesicle-associated membrane protein-7

ASJC Scopus subject areas

  • Immunology

Cite this

A critical role for vesicle-associated membrane protein-7 in exocytosis from human eosinophils and neutrophils. / Logan, M. R.; Lacy, P.; Odemuyiwa, S. O.; Steward, M.; Davoine, F.; Kita, Hirohito; Moqbel, R.

In: Allergy: European Journal of Allergy and Clinical Immunology, Vol. 61, No. 6, 06.2006, p. 777-784.

Research output: Contribution to journalArticle

Logan, M. R. ; Lacy, P. ; Odemuyiwa, S. O. ; Steward, M. ; Davoine, F. ; Kita, Hirohito ; Moqbel, R. / A critical role for vesicle-associated membrane protein-7 in exocytosis from human eosinophils and neutrophils. In: Allergy: European Journal of Allergy and Clinical Immunology. 2006 ; Vol. 61, No. 6. pp. 777-784.
@article{3379c149dce740ccbc76717b07620d1c,
title = "A critical role for vesicle-associated membrane protein-7 in exocytosis from human eosinophils and neutrophils",
abstract = "Background: Granulocyte exocytosis is proposed to be critically dependent on the interaction of soluble N-ethylmaleimide-sensitive factor attachment protein (SNAP) receptors (SNAREs) located on granules/vesicles (v-SNAREs) and plasma membrane (t-SNAREs). Previous studies indicated that the v-SNARE, vesicle-associated membrane protein (VAMP)-2, as well as t-SNAREs (SNAP-23, syntaxin-4 and -6) are implicated in exocytosis from human granulocytes. Vesicle-associated membrane proteins-7 and -8 have been implicated in endosome/lysosome trafficking, however, their role in granulocyte exocytosis remains obscure. Objective: We sought to investigate the expression and functional role of SNARE isoforms in the secretion of different granule-derived mediators in human eosinophils and neutrophils. Methods: The expression of SNAREs was determined by subcellular fractionation and flow cytometry. SNARE-specific antibodies were examined for their ability to impair mediator release from permeabilized eosinophils and neutrophils. Results: Vesicle-associated membrane proteins-7 and -8 were localized to granule and membrane-enriched fractions in eosinophils and neutrophils, whereas syntaxin-6 was not detectable. In permeabilized cells, anti-VAMP-7, but not anti-VAMP-8, antibody impaired the secretion of all mediators examined (in eosinophils, eosinophil peroxidase and eosinophil-derived neurotoxin; in neutrophils, myeloperoxidase, lactoferrin and matrix metalloprotease-9) in a dose-dependent manner. In contrast, anti-VAMP-2 modestly and selectively impaired secretion from small granules and vesicles. Syntaxin-4, but not syntaxin-6, was found to interact with SNAP-23 and was partially involved in mediator secretion from multiple compartments. Conclusion: Our observations indicate for the first time a critical role for VAMP-7 in both eosinophil and neutrophil mediator release.",
keywords = "Exocytosis, Granulocytes, Soluble N-ethylmaleimide-sensitive factor attachment protein receptors, Vesicle-associated membrane protein-7",
author = "Logan, {M. R.} and P. Lacy and Odemuyiwa, {S. O.} and M. Steward and F. Davoine and Hirohito Kita and R. Moqbel",
year = "2006",
month = "6",
doi = "10.1111/j.1398-9995.2006.01089.x",
language = "English (US)",
volume = "61",
pages = "777--784",
journal = "Allergy: European Journal of Allergy and Clinical Immunology",
issn = "0105-4538",
publisher = "Wiley-Blackwell",
number = "6",

}

TY - JOUR

T1 - A critical role for vesicle-associated membrane protein-7 in exocytosis from human eosinophils and neutrophils

AU - Logan, M. R.

AU - Lacy, P.

AU - Odemuyiwa, S. O.

AU - Steward, M.

AU - Davoine, F.

AU - Kita, Hirohito

AU - Moqbel, R.

PY - 2006/6

Y1 - 2006/6

N2 - Background: Granulocyte exocytosis is proposed to be critically dependent on the interaction of soluble N-ethylmaleimide-sensitive factor attachment protein (SNAP) receptors (SNAREs) located on granules/vesicles (v-SNAREs) and plasma membrane (t-SNAREs). Previous studies indicated that the v-SNARE, vesicle-associated membrane protein (VAMP)-2, as well as t-SNAREs (SNAP-23, syntaxin-4 and -6) are implicated in exocytosis from human granulocytes. Vesicle-associated membrane proteins-7 and -8 have been implicated in endosome/lysosome trafficking, however, their role in granulocyte exocytosis remains obscure. Objective: We sought to investigate the expression and functional role of SNARE isoforms in the secretion of different granule-derived mediators in human eosinophils and neutrophils. Methods: The expression of SNAREs was determined by subcellular fractionation and flow cytometry. SNARE-specific antibodies were examined for their ability to impair mediator release from permeabilized eosinophils and neutrophils. Results: Vesicle-associated membrane proteins-7 and -8 were localized to granule and membrane-enriched fractions in eosinophils and neutrophils, whereas syntaxin-6 was not detectable. In permeabilized cells, anti-VAMP-7, but not anti-VAMP-8, antibody impaired the secretion of all mediators examined (in eosinophils, eosinophil peroxidase and eosinophil-derived neurotoxin; in neutrophils, myeloperoxidase, lactoferrin and matrix metalloprotease-9) in a dose-dependent manner. In contrast, anti-VAMP-2 modestly and selectively impaired secretion from small granules and vesicles. Syntaxin-4, but not syntaxin-6, was found to interact with SNAP-23 and was partially involved in mediator secretion from multiple compartments. Conclusion: Our observations indicate for the first time a critical role for VAMP-7 in both eosinophil and neutrophil mediator release.

AB - Background: Granulocyte exocytosis is proposed to be critically dependent on the interaction of soluble N-ethylmaleimide-sensitive factor attachment protein (SNAP) receptors (SNAREs) located on granules/vesicles (v-SNAREs) and plasma membrane (t-SNAREs). Previous studies indicated that the v-SNARE, vesicle-associated membrane protein (VAMP)-2, as well as t-SNAREs (SNAP-23, syntaxin-4 and -6) are implicated in exocytosis from human granulocytes. Vesicle-associated membrane proteins-7 and -8 have been implicated in endosome/lysosome trafficking, however, their role in granulocyte exocytosis remains obscure. Objective: We sought to investigate the expression and functional role of SNARE isoforms in the secretion of different granule-derived mediators in human eosinophils and neutrophils. Methods: The expression of SNAREs was determined by subcellular fractionation and flow cytometry. SNARE-specific antibodies were examined for their ability to impair mediator release from permeabilized eosinophils and neutrophils. Results: Vesicle-associated membrane proteins-7 and -8 were localized to granule and membrane-enriched fractions in eosinophils and neutrophils, whereas syntaxin-6 was not detectable. In permeabilized cells, anti-VAMP-7, but not anti-VAMP-8, antibody impaired the secretion of all mediators examined (in eosinophils, eosinophil peroxidase and eosinophil-derived neurotoxin; in neutrophils, myeloperoxidase, lactoferrin and matrix metalloprotease-9) in a dose-dependent manner. In contrast, anti-VAMP-2 modestly and selectively impaired secretion from small granules and vesicles. Syntaxin-4, but not syntaxin-6, was found to interact with SNAP-23 and was partially involved in mediator secretion from multiple compartments. Conclusion: Our observations indicate for the first time a critical role for VAMP-7 in both eosinophil and neutrophil mediator release.

KW - Exocytosis

KW - Granulocytes

KW - Soluble N-ethylmaleimide-sensitive factor attachment protein receptors

KW - Vesicle-associated membrane protein-7

UR - http://www.scopus.com/inward/record.url?scp=33744458462&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33744458462&partnerID=8YFLogxK

U2 - 10.1111/j.1398-9995.2006.01089.x

DO - 10.1111/j.1398-9995.2006.01089.x

M3 - Article

VL - 61

SP - 777

EP - 784

JO - Allergy: European Journal of Allergy and Clinical Immunology

JF - Allergy: European Journal of Allergy and Clinical Immunology

SN - 0105-4538

IS - 6

ER -