A CD8 T-cell epitope variant enhances immune targeting to a recombinant picornavirus vaccine antigen

Michael P. Bell, Danielle N. Renner, Aaron J. Johnson, Kevin D. Pavelko

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Recombinant virus vaccines are often less effective due to immunodominant responses against endogenous vector antigens. However, the use of small RNA virus vectors provides an opportunity to limit host exposure to endogenous virus antigens and focus immune responses on the desired vaccine antigen. Using the Daniel's strain of Theiler's murine encephalomyelitis virus, we have identified strategies to modulate responses to endogenous viral proteins by manipulating the host CD8+ T-cell repertoire prior to infection or through the use of mutations introduced into the virus genome. Both of these approaches enhance responses to vaccine antigens introduced into the picornavirus. However, the use of mutant immunodominant epitopes provides an opportunity for enhancing vaccine responses without further manipulation of the host. Using this strategy, we demonstrate that modification of the consensus MHC class I anchor residue within the virus genome can promote enhanced immunity to foreign antigens and self-antigens embedded in the virus genome.

Original languageEnglish (US)
Pages (from-to)361-366
Number of pages6
JournalViral Immunology
Volume27
Issue number7
DOIs
StatePublished - Sep 1 2014

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Picornaviridae
Synthetic Vaccines
T-Lymphocyte Epitopes
Viruses
Antigens
Vaccines
Genome
Theilovirus
Immunodominant Epitopes
RNA Viruses
Histocompatibility Antigens Class II
Autoantigens
Viral Proteins
Immunity
T-Lymphocytes
Mutation
Infection

ASJC Scopus subject areas

  • Immunology
  • Virology
  • Molecular Medicine

Cite this

A CD8 T-cell epitope variant enhances immune targeting to a recombinant picornavirus vaccine antigen. / Bell, Michael P.; Renner, Danielle N.; Johnson, Aaron J.; Pavelko, Kevin D.

In: Viral Immunology, Vol. 27, No. 7, 01.09.2014, p. 361-366.

Research output: Contribution to journalArticle

Bell, Michael P. ; Renner, Danielle N. ; Johnson, Aaron J. ; Pavelko, Kevin D. / A CD8 T-cell epitope variant enhances immune targeting to a recombinant picornavirus vaccine antigen. In: Viral Immunology. 2014 ; Vol. 27, No. 7. pp. 361-366.
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