1,25-dihydroxyvitamin d3 regulates the expression of N-myc, c-myc, protein kinase c, and transforming growth factor-β2 in neuroblastoma cells

Timothy D. Veenstra, Anthony J. Windebank, Rajiv Kumar

Research output: Contribution to journalArticle

32 Scopus citations

Abstract

1α,25-Dihydroxyvitamin D3 (1,25(OH)2D3) alters the proliferation of neuroblastoma cells in culture in part via a nerve growth factor (NGF)-mediated pathway. This suggests that factors other than NGF also play a role in the growth arrest induced by 1,25(OH)2D3. To more fully characterize the effect of 1,25(OH)2D3 on neuroblastoma cells, we treated the cells with 10-8 M 1,25(OH)2D3 and examined the cells for changes in the expression of N-myc, c-myc, transforming growth factor-β2 (TGF-β2), and protein kinase C (PKC) activity. Our results show that 1,25(OH)2D3 causes a decrease in the expression of N-myc and c-myc, as well as a twofold increase in total PKC activity and a dose-dependent increase in TGF-β2 expression. These results show that 1,25(OH)2D3 regulates the expression of growth-regulatory factors other than NGF in neuroblastoma cells and that 1,25(OH)2D3 influences the growth of neural cells via multiple growth regulatory pathways.

Original languageEnglish (US)
Pages (from-to)15-18
Number of pages4
JournalBiochemical and Biophysical Research Communications
Volume235
Issue number1
DOIs
StatePublished - Jun 9 1997

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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