1,25-dihydroxyvitamin d3 regulates the expression of N-myc, c-myc, protein kinase c, and transforming growth factor-β2 in neuroblastoma cells

Timothy D. Veenstra, Anthony John Windebank, Rajiv Kumar

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

1α,25-Dihydroxyvitamin D3 (1,25(OH)2D3) alters the proliferation of neuroblastoma cells in culture in part via a nerve growth factor (NGF)-mediated pathway. This suggests that factors other than NGF also play a role in the growth arrest induced by 1,25(OH)2D3. To more fully characterize the effect of 1,25(OH)2D3 on neuroblastoma cells, we treated the cells with 10-8 M 1,25(OH)2D3 and examined the cells for changes in the expression of N-myc, c-myc, transforming growth factor-β2 (TGF-β2), and protein kinase C (PKC) activity. Our results show that 1,25(OH)2D3 causes a decrease in the expression of N-myc and c-myc, as well as a twofold increase in total PKC activity and a dose-dependent increase in TGF-β2 expression. These results show that 1,25(OH)2D3 regulates the expression of growth-regulatory factors other than NGF in neuroblastoma cells and that 1,25(OH)2D3 influences the growth of neural cells via multiple growth regulatory pathways.

Original languageEnglish (US)
Pages (from-to)15-18
Number of pages4
JournalBiochemical and Biophysical Research Communications
Volume235
Issue number1
DOIs
StatePublished - Jun 9 1997

Fingerprint

Proto-Oncogene Proteins c-myc
Calcitriol
Transforming Growth Factors
Neuroblastoma
Protein Kinases
Nerve Growth Factor
Protein Kinase C
Growth
Cells
Intercellular Signaling Peptides and Proteins
Cell Culture Techniques

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

@article{8f7b353428794f9d80b33e30e960a59b,
title = "1,25-dihydroxyvitamin d3 regulates the expression of N-myc, c-myc, protein kinase c, and transforming growth factor-β2 in neuroblastoma cells",
abstract = "1α,25-Dihydroxyvitamin D3 (1,25(OH)2D3) alters the proliferation of neuroblastoma cells in culture in part via a nerve growth factor (NGF)-mediated pathway. This suggests that factors other than NGF also play a role in the growth arrest induced by 1,25(OH)2D3. To more fully characterize the effect of 1,25(OH)2D3 on neuroblastoma cells, we treated the cells with 10-8 M 1,25(OH)2D3 and examined the cells for changes in the expression of N-myc, c-myc, transforming growth factor-β2 (TGF-β2), and protein kinase C (PKC) activity. Our results show that 1,25(OH)2D3 causes a decrease in the expression of N-myc and c-myc, as well as a twofold increase in total PKC activity and a dose-dependent increase in TGF-β2 expression. These results show that 1,25(OH)2D3 regulates the expression of growth-regulatory factors other than NGF in neuroblastoma cells and that 1,25(OH)2D3 influences the growth of neural cells via multiple growth regulatory pathways.",
author = "Veenstra, {Timothy D.} and Windebank, {Anthony John} and Rajiv Kumar",
year = "1997",
month = "6",
day = "9",
doi = "10.1006/bbrc.1997.6718",
language = "English (US)",
volume = "235",
pages = "15--18",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Academic Press Inc.",
number = "1",

}

TY - JOUR

T1 - 1,25-dihydroxyvitamin d3 regulates the expression of N-myc, c-myc, protein kinase c, and transforming growth factor-β2 in neuroblastoma cells

AU - Veenstra, Timothy D.

AU - Windebank, Anthony John

AU - Kumar, Rajiv

PY - 1997/6/9

Y1 - 1997/6/9

N2 - 1α,25-Dihydroxyvitamin D3 (1,25(OH)2D3) alters the proliferation of neuroblastoma cells in culture in part via a nerve growth factor (NGF)-mediated pathway. This suggests that factors other than NGF also play a role in the growth arrest induced by 1,25(OH)2D3. To more fully characterize the effect of 1,25(OH)2D3 on neuroblastoma cells, we treated the cells with 10-8 M 1,25(OH)2D3 and examined the cells for changes in the expression of N-myc, c-myc, transforming growth factor-β2 (TGF-β2), and protein kinase C (PKC) activity. Our results show that 1,25(OH)2D3 causes a decrease in the expression of N-myc and c-myc, as well as a twofold increase in total PKC activity and a dose-dependent increase in TGF-β2 expression. These results show that 1,25(OH)2D3 regulates the expression of growth-regulatory factors other than NGF in neuroblastoma cells and that 1,25(OH)2D3 influences the growth of neural cells via multiple growth regulatory pathways.

AB - 1α,25-Dihydroxyvitamin D3 (1,25(OH)2D3) alters the proliferation of neuroblastoma cells in culture in part via a nerve growth factor (NGF)-mediated pathway. This suggests that factors other than NGF also play a role in the growth arrest induced by 1,25(OH)2D3. To more fully characterize the effect of 1,25(OH)2D3 on neuroblastoma cells, we treated the cells with 10-8 M 1,25(OH)2D3 and examined the cells for changes in the expression of N-myc, c-myc, transforming growth factor-β2 (TGF-β2), and protein kinase C (PKC) activity. Our results show that 1,25(OH)2D3 causes a decrease in the expression of N-myc and c-myc, as well as a twofold increase in total PKC activity and a dose-dependent increase in TGF-β2 expression. These results show that 1,25(OH)2D3 regulates the expression of growth-regulatory factors other than NGF in neuroblastoma cells and that 1,25(OH)2D3 influences the growth of neural cells via multiple growth regulatory pathways.

UR - http://www.scopus.com/inward/record.url?scp=0031560921&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031560921&partnerID=8YFLogxK

U2 - 10.1006/bbrc.1997.6718

DO - 10.1006/bbrc.1997.6718

M3 - Article

C2 - 9196027

AN - SCOPUS:0031560921

VL - 235

SP - 15

EP - 18

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 1

ER -