TY - JOUR
T1 - 1,25-dihydroxyvitamin d3 regulates the expression of N-myc, c-myc, protein kinase c, and transforming growth factor-β2 in neuroblastoma cells
AU - Veenstra, Timothy D.
AU - Windebank, Anthony J.
AU - Kumar, Rajiv
N1 - Funding Information:
1This research was supported by NIH Grant DK-25409 to R.K. 2To whom correspondence should be addressed at Mayo Clinic, 200 First Street SW, 911A Guggenheim Building, Rochester, MN 55905. Fax: (507) 266-4710. E-mail: rkumar@mayo.edu.
PY - 1997/6/9
Y1 - 1997/6/9
N2 - 1α,25-Dihydroxyvitamin D3 (1,25(OH)2D3) alters the proliferation of neuroblastoma cells in culture in part via a nerve growth factor (NGF)-mediated pathway. This suggests that factors other than NGF also play a role in the growth arrest induced by 1,25(OH)2D3. To more fully characterize the effect of 1,25(OH)2D3 on neuroblastoma cells, we treated the cells with 10-8 M 1,25(OH)2D3 and examined the cells for changes in the expression of N-myc, c-myc, transforming growth factor-β2 (TGF-β2), and protein kinase C (PKC) activity. Our results show that 1,25(OH)2D3 causes a decrease in the expression of N-myc and c-myc, as well as a twofold increase in total PKC activity and a dose-dependent increase in TGF-β2 expression. These results show that 1,25(OH)2D3 regulates the expression of growth-regulatory factors other than NGF in neuroblastoma cells and that 1,25(OH)2D3 influences the growth of neural cells via multiple growth regulatory pathways.
AB - 1α,25-Dihydroxyvitamin D3 (1,25(OH)2D3) alters the proliferation of neuroblastoma cells in culture in part via a nerve growth factor (NGF)-mediated pathway. This suggests that factors other than NGF also play a role in the growth arrest induced by 1,25(OH)2D3. To more fully characterize the effect of 1,25(OH)2D3 on neuroblastoma cells, we treated the cells with 10-8 M 1,25(OH)2D3 and examined the cells for changes in the expression of N-myc, c-myc, transforming growth factor-β2 (TGF-β2), and protein kinase C (PKC) activity. Our results show that 1,25(OH)2D3 causes a decrease in the expression of N-myc and c-myc, as well as a twofold increase in total PKC activity and a dose-dependent increase in TGF-β2 expression. These results show that 1,25(OH)2D3 regulates the expression of growth-regulatory factors other than NGF in neuroblastoma cells and that 1,25(OH)2D3 influences the growth of neural cells via multiple growth regulatory pathways.
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U2 - 10.1006/bbrc.1997.6718
DO - 10.1006/bbrc.1997.6718
M3 - Article
C2 - 9196027
AN - SCOPUS:0031560921
SN - 0006-291X
VL - 235
SP - 15
EP - 18
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 1
ER -