1,2,3,4,6-penta-O-galloyl-beta-d-glucose attenuates renal cell migration, hyaluronan expression, and crystal adhesion

Jae Ho Lee, Matthew Yehl, Kwang Seok Ahn, Sung Hoon Kim, John C. Lieske

Research output: Contribution to journalArticle

14 Scopus citations

Abstract

Calcium oxalate monohydrate (COM) crystals bind avidly to the surface of proliferating and migrating renal endothelial cells, and oxalate-induced peroxidative injury can promote crystal attachment to renal epithelial cells. 1,2,3,4,6-penta-O-galloyl-beta-d-glucose (PGG), isolated from a traditional herbal remedy, inhibits vascular endothelial growth factor (VEGF) stimulated proliferation and migration of human umbilical vein endothelial cells (HUVECs) and has antioxidant activity. This study was performed to determine if PGG altered calcium oxalate monohydrate (COM) crystal adhesion to cells, perhaps via a change in cell surface properties. PGG significantly decreased COM crystal adhesion to cultured MDCK I cells at a low concentration (< 10 μM) which was not cytotoxic. PGG exerted anti-adhesion effects whether cells or crystals were pre-coated. PGG also inhibited cell migration after scrape-wounding, decreased subsequent adhesion of crystals to proliferating and migrating cells, and decreased expression of the crystal binding molecule hyaluronan. These findings suggest that PGG represents a potential urolithiasis prevention compound. Anti-crystal adhesion effects appear multifaceted involving crystal coating by PGG, as well as decreased cell migration and the associated surface expression of hyaluronan. The latter represents a novel mechanism of nephrolithiasis prevention.

Original languageEnglish (US)
Pages (from-to)32-37
Number of pages6
JournalEuropean Journal of Pharmacology
Volume606
Issue number1-3
DOIs
StatePublished - Mar 15 2009

Keywords

  • Calcium oxalate
  • Cell movement
  • Chinese herbal drugs
  • Hyaluronic acid

ASJC Scopus subject areas

  • Pharmacology

Fingerprint Dive into the research topics of '1,2,3,4,6-penta-O-galloyl-beta-d-glucose attenuates renal cell migration, hyaluronan expression, and crystal adhesion'. Together they form a unique fingerprint.

  • Cite this