1,2-bis(2-aminophenoxy)ethane-N,N,N′N′-tetraacetic acid (BAPTA-AM) inhibits caffeine-induced apoptosis in human neuroblastoma cells

Mi Hyeon Jang, Min Chul Shin, Young Wuk Cho, Hyung Hwan Baik, Sung Soo Kim, Eun Gu Hwang, Chang Ju Kim

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

In the present study, it was investigated whether 1,2-bis(2-aminophenoxy) ethane-N,N,N′N′-tetraacetic acid (BAPTA-AM), an intracellular Ca2+ chelator, possesses protective effect against caffeine-induced apoptosis in the central nervous system. Through morphological and biochemical analyses, cells treated with caffeine exhibited several apoptotic features. On the other hand, cells treated with caffeine and BAPTA-AM, showed decreased occurrence of apoptotic features. In addition, it was shown that BAPTA-AM treatment inhibits caffeine-induced increase of caspase-3 enzyme activity. These results show that caffeine induces apoptotic death in human SK-N-MC neuroblastoma cells and BAPTA-AM prevents apoptosis by attenuating caffeine-induced caspase-3 activation.

Original languageEnglish (US)
Pages (from-to)189-192
Number of pages4
JournalNeuroscience Letters
Volume358
Issue number3
DOIs
StatePublished - Apr 1 2004

Keywords

  • Apoptosis
  • BAPTA-AM
  • Caffeine
  • Neuroblastoma

ASJC Scopus subject areas

  • Neuroscience(all)

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