1,2-bis(2-aminophenoxy)ethane-N,N,N′N′-tetraacetic acid (BAPTA-AM) inhibits caffeine-induced apoptosis in human neuroblastoma cells

Mi Hyeon Jang; Min Chul Shin; Young Wuk Cho; Hyung Hwan Baik; Sung Soo Kim; Eun Gu Hwang; Chang Ju Kim

doi:10.1016/j.neulet.2004.01.040

1,2-bis(2-aminophenoxy)ethane-N,N,N′N′-tetraacetic acid (BAPTA-AM) inhibits caffeine-induced apoptosis in human neuroblastoma cells

Mi Hyeon Jang, Min Chul Shin, Young Wuk Cho, Hyung Hwan Baik, Sung Soo Kim, Eun Gu Hwang, Chang Ju Kim

Neurosurgery

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4 Scopus citations

Abstract

In the present study, it was investigated whether 1,2-bis(2-aminophenoxy) ethane-N,N,N′N′-tetraacetic acid (BAPTA-AM), an intracellular Ca²⁺ chelator, possesses protective effect against caffeine-induced apoptosis in the central nervous system. Through morphological and biochemical analyses, cells treated with caffeine exhibited several apoptotic features. On the other hand, cells treated with caffeine and BAPTA-AM, showed decreased occurrence of apoptotic features. In addition, it was shown that BAPTA-AM treatment inhibits caffeine-induced increase of caspase-3 enzyme activity. These results show that caffeine induces apoptotic death in human SK-N-MC neuroblastoma cells and BAPTA-AM prevents apoptosis by attenuating caffeine-induced caspase-3 activation.

Original language	English (US)
Pages (from-to)	189-192
Number of pages	4
Journal	Neuroscience Letters
Volume	358
Issue number	3
DOIs	https://doi.org/10.1016/j.neulet.2004.01.040
State	Published - Apr 1 2004

Keywords

Apoptosis
BAPTA-AM
Caffeine
Neuroblastoma

ASJC Scopus subject areas

General Neuroscience

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10.1016/j.neulet.2004.01.040

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@article{4dd08db2e5e54ea6888b530c0e00bc44,

title = "1,2-bis(2-aminophenoxy)ethane-N,N,N′N′-tetraacetic acid (BAPTA-AM) inhibits caffeine-induced apoptosis in human neuroblastoma cells",

abstract = "In the present study, it was investigated whether 1,2-bis(2-aminophenoxy) ethane-N,N,N′N′-tetraacetic acid (BAPTA-AM), an intracellular Ca2+ chelator, possesses protective effect against caffeine-induced apoptosis in the central nervous system. Through morphological and biochemical analyses, cells treated with caffeine exhibited several apoptotic features. On the other hand, cells treated with caffeine and BAPTA-AM, showed decreased occurrence of apoptotic features. In addition, it was shown that BAPTA-AM treatment inhibits caffeine-induced increase of caspase-3 enzyme activity. These results show that caffeine induces apoptotic death in human SK-N-MC neuroblastoma cells and BAPTA-AM prevents apoptosis by attenuating caffeine-induced caspase-3 activation.",

keywords = "Apoptosis, BAPTA-AM, Caffeine, Neuroblastoma",

author = "Jang, {Mi Hyeon} and Shin, {Min Chul} and Cho, {Young Wuk} and Baik, {Hyung Hwan} and Kim, {Sung Soo} and Hwang, {Eun Gu} and Kim, {Chang Ju}",

note = "Funding Information: This work was supported by grant from the Korea Science & Engineering Foundation (R13-2002-020-01001-0). ",

year = "2004",

month = apr,

day = "1",

doi = "10.1016/j.neulet.2004.01.040",

language = "English (US)",

volume = "358",

pages = "189--192",

journal = "Neuroscience Letters",

issn = "0304-3940",

publisher = "Elsevier Ireland Ltd",

number = "3",

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TY - JOUR

T1 - 1,2-bis(2-aminophenoxy)ethane-N,N,N′N′-tetraacetic acid (BAPTA-AM) inhibits caffeine-induced apoptosis in human neuroblastoma cells

AU - Jang, Mi Hyeon

AU - Shin, Min Chul

AU - Cho, Young Wuk

AU - Baik, Hyung Hwan

AU - Kim, Sung Soo

AU - Hwang, Eun Gu

AU - Kim, Chang Ju

N1 - Funding Information: This work was supported by grant from the Korea Science & Engineering Foundation (R13-2002-020-01001-0).

PY - 2004/4/1

Y1 - 2004/4/1

N2 - In the present study, it was investigated whether 1,2-bis(2-aminophenoxy) ethane-N,N,N′N′-tetraacetic acid (BAPTA-AM), an intracellular Ca2+ chelator, possesses protective effect against caffeine-induced apoptosis in the central nervous system. Through morphological and biochemical analyses, cells treated with caffeine exhibited several apoptotic features. On the other hand, cells treated with caffeine and BAPTA-AM, showed decreased occurrence of apoptotic features. In addition, it was shown that BAPTA-AM treatment inhibits caffeine-induced increase of caspase-3 enzyme activity. These results show that caffeine induces apoptotic death in human SK-N-MC neuroblastoma cells and BAPTA-AM prevents apoptosis by attenuating caffeine-induced caspase-3 activation.

AB - In the present study, it was investigated whether 1,2-bis(2-aminophenoxy) ethane-N,N,N′N′-tetraacetic acid (BAPTA-AM), an intracellular Ca2+ chelator, possesses protective effect against caffeine-induced apoptosis in the central nervous system. Through morphological and biochemical analyses, cells treated with caffeine exhibited several apoptotic features. On the other hand, cells treated with caffeine and BAPTA-AM, showed decreased occurrence of apoptotic features. In addition, it was shown that BAPTA-AM treatment inhibits caffeine-induced increase of caspase-3 enzyme activity. These results show that caffeine induces apoptotic death in human SK-N-MC neuroblastoma cells and BAPTA-AM prevents apoptosis by attenuating caffeine-induced caspase-3 activation.

KW - Apoptosis

KW - BAPTA-AM

KW - Caffeine

KW - Neuroblastoma

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U2 - 10.1016/j.neulet.2004.01.040

DO - 10.1016/j.neulet.2004.01.040

M3 - Article

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AN - SCOPUS:1642317167

SN - 0304-3940

VL - 358

SP - 189

EP - 192

JO - Neuroscience Letters

JF - Neuroscience Letters

IS - 3

ER -

1,2-bis(2-aminophenoxy)ethane-N,N,N′N′-tetraacetic acid (BAPTA-AM) inhibits caffeine-induced apoptosis in human neuroblastoma cells

Abstract

Keywords

ASJC Scopus subject areas

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