Separation of anti-Ia (I-region associated antigens) from anti-H-2 antibodies in complex sera, by absorption on blood platelets. Description of 3 new Ia specificities

J. Colombani, M. Colombani, D. C. Shreffler, C. David

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

H 2 antigens are expressed in substantial amounts on murine blood platelets (for H 2 antigenic content 1 lymphocyte 50 platelets) whereas Ia antigens are probably not expressed at all (minimal Ia antigenic content more than 35 times lower than for H 2). This property of blood platelets makes them very useful for the selective absorption of anti H 2 antibodies from complex sera and for the preparation of specific anti Ia antibodies from such sera. In 20 sera produced against the complete H 2 complex, 12 sera contained anti Ia antibodies beside the expected anti H 2 antibodies. In two sera, separation of the anti Ia antibodies was easily obtained by absorption of the anti H 2 antibodies on platelets. The analysis of one serum (C3H.Q x B10.D2) anti C3H [(q x d) anti k] showed that, in addition to the expected anti H 2.23 and anti Ia.2 antibodies, it contained at least three other Ia antibodies, separable by absorption on lymphocytes, which recognized three antigens: Ia.17, determined by the haplotypes k, f, s, r, j; Ia.18, determined by the haplotypes k, f, s; and Ia.19, determined by the haplotypes k and r. The genes are located in the I A and/or I B subregions of the H 2 complex.

Original languageEnglish (US)
Pages (from-to)74-85
Number of pages12
JournalTissue Antigens
Volume7
Issue number2
StatePublished - 1976
Externally publishedYes

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Blood Platelets
Antigens
Serum
Haplotypes
Anti-Idiotypic Antibodies
H-2 Antigens
Lymphocytes
Antibodies
Histocompatibility Antigens Class II
anti-H-2 antibodies
Genes

ASJC Scopus subject areas

  • Cell Biology
  • Immunology

Cite this

Separation of anti-Ia (I-region associated antigens) from anti-H-2 antibodies in complex sera, by absorption on blood platelets. Description of 3 new Ia specificities. / Colombani, J.; Colombani, M.; Shreffler, D. C.; David, C.

In: Tissue Antigens, Vol. 7, No. 2, 1976, p. 74-85.

Research output: Contribution to journalArticle

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abstract = "H 2 antigens are expressed in substantial amounts on murine blood platelets (for H 2 antigenic content 1 lymphocyte 50 platelets) whereas Ia antigens are probably not expressed at all (minimal Ia antigenic content more than 35 times lower than for H 2). This property of blood platelets makes them very useful for the selective absorption of anti H 2 antibodies from complex sera and for the preparation of specific anti Ia antibodies from such sera. In 20 sera produced against the complete H 2 complex, 12 sera contained anti Ia antibodies beside the expected anti H 2 antibodies. In two sera, separation of the anti Ia antibodies was easily obtained by absorption of the anti H 2 antibodies on platelets. The analysis of one serum (C3H.Q x B10.D2) anti C3H [(q x d) anti k] showed that, in addition to the expected anti H 2.23 and anti Ia.2 antibodies, it contained at least three other Ia antibodies, separable by absorption on lymphocytes, which recognized three antigens: Ia.17, determined by the haplotypes k, f, s, r, j; Ia.18, determined by the haplotypes k, f, s; and Ia.19, determined by the haplotypes k and r. The genes are located in the I A and/or I B subregions of the H 2 complex.",
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AU - Shreffler, D. C.

AU - David, C.

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AB - H 2 antigens are expressed in substantial amounts on murine blood platelets (for H 2 antigenic content 1 lymphocyte 50 platelets) whereas Ia antigens are probably not expressed at all (minimal Ia antigenic content more than 35 times lower than for H 2). This property of blood platelets makes them very useful for the selective absorption of anti H 2 antibodies from complex sera and for the preparation of specific anti Ia antibodies from such sera. In 20 sera produced against the complete H 2 complex, 12 sera contained anti Ia antibodies beside the expected anti H 2 antibodies. In two sera, separation of the anti Ia antibodies was easily obtained by absorption of the anti H 2 antibodies on platelets. The analysis of one serum (C3H.Q x B10.D2) anti C3H [(q x d) anti k] showed that, in addition to the expected anti H 2.23 and anti Ia.2 antibodies, it contained at least three other Ia antibodies, separable by absorption on lymphocytes, which recognized three antigens: Ia.17, determined by the haplotypes k, f, s, r, j; Ia.18, determined by the haplotypes k, f, s; and Ia.19, determined by the haplotypes k and r. The genes are located in the I A and/or I B subregions of the H 2 complex.

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