TY - JOUR
T1 - Procalcitonin NH2‐terminal cleavage peptide has no mitogenic effect on normal human osteoblast‐like cells
AU - Hassager, Christian
AU - Bonde, Susan K.
AU - Anderson, Marlys A.
AU - Rink, H.
AU - Spelsberg, Thomas C.
AU - Riggs, B. Lawrence
PY - 1991/5
Y1 - 1991/5
N2 - The NH2‐terminal cleavage peptide of procalcitonin (N‐proCT) recently was reported to be a bone cell mitogen (Burns DM et al., Proc Natl Acad Sci USA 86:9519–9523, 1989). We have investigated the effect of N‐proCT on the proliferation of normal human cells that have the phenotype of mature osteoblasts (hOB cells). N‐proCT treatment for 24, 48, or 96 h in concentrations from 1 nM to 1 μM did not significantly increase [3H]thymidine uptake (means ranged from ‐19% to 38% of control, no significant differences) in hOB cells (6–10 cell strains per experiment) plated at four different densities. However, the hOB cells responded significantly to treatment with transforming growth factor β (3 ng/ml), bovine insulin (300 μg/ml), or 30% fetal calf serum, which were included in all experiments as positive controls. The [3H]thymidine uptake data were confirmed in a direct cell count experiment tested at 96 h. Thus our data do not support the hypothesis that N‐proCT is a potent mitogen for normal human osteoblasts.
AB - The NH2‐terminal cleavage peptide of procalcitonin (N‐proCT) recently was reported to be a bone cell mitogen (Burns DM et al., Proc Natl Acad Sci USA 86:9519–9523, 1989). We have investigated the effect of N‐proCT on the proliferation of normal human cells that have the phenotype of mature osteoblasts (hOB cells). N‐proCT treatment for 24, 48, or 96 h in concentrations from 1 nM to 1 μM did not significantly increase [3H]thymidine uptake (means ranged from ‐19% to 38% of control, no significant differences) in hOB cells (6–10 cell strains per experiment) plated at four different densities. However, the hOB cells responded significantly to treatment with transforming growth factor β (3 ng/ml), bovine insulin (300 μg/ml), or 30% fetal calf serum, which were included in all experiments as positive controls. The [3H]thymidine uptake data were confirmed in a direct cell count experiment tested at 96 h. Thus our data do not support the hypothesis that N‐proCT is a potent mitogen for normal human osteoblasts.
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U2 - 10.1002/jbmr.5650060510
DO - 10.1002/jbmr.5650060510
M3 - Article
C2 - 2068955
AN - SCOPUS:0025923066
SN - 0884-0431
VL - 6
SP - 489
EP - 493
JO - Journal of Bone and Mineral Research
JF - Journal of Bone and Mineral Research
IS - 5
ER -