Platelet glycoprotein IIb-IIIaIIbβ3 integrin) confers fibrinogen- and activation-dependent aggregation on heterologous cells

Mony M. Frojmovic, Timothy E. O'Toole, Edward F. Plow, Joseph C Loftus, Mark H. Ginsberg

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

To analyze molecular mechanisms of platelet aggregation, we have studied the aggregation of Chinese hamster ovary (CHO) cells expressing between 1 and 4 × 105 recombinant human glycoprotein (GP) IIb-IIIa molecules per cell (A5 cells). These cells aggregated as measured by the disappearance of single cells during rotary agitation. Aggregation was dependent on the presence of extracellular fibrinogen (≈500 nmol/L) and divalent cations, and required prior activation of the GPIIb-IIIa. A synthetic peptide (GRGDSP) and monoclonal anti-GPIIb-IIIa antibody (2G12) that block platelet aggregation also blocked aggregation of these cells. Parent CHO cells or those expressing recombinant GPIIb-IIIa containing a point mutation that causes variant thrombasthenia both failed to aggregate when stimulated in the presence of fibrinogen. These data show that GPIIb-IIIa is the only unique platelet surface component required for aggregation.

Original languageEnglish (US)
Pages (from-to)369-376
Number of pages8
JournalBlood
Volume78
Issue number2
StatePublished - Jul 15 1991
Externally publishedYes

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Integrin beta3
Platelet Membrane Glycoproteins
Platelet Glycoprotein GPIIb-IIIa Complex
Integrins
Fibrinogen
Agglomeration
Chemical activation
Platelets
Cricetulus
Platelet Aggregation
glycyl-arginyl-glycyl-aspartyl-seryl-proline
Ovary
Thrombasthenia
Cell Aggregation
Divalent Cations
Point Mutation
Blood Platelets
Cells
Peptides
Molecules

ASJC Scopus subject areas

  • Hematology

Cite this

Platelet glycoprotein IIb-IIIaIIbβ3 integrin) confers fibrinogen- and activation-dependent aggregation on heterologous cells. / Frojmovic, Mony M.; O'Toole, Timothy E.; Plow, Edward F.; Loftus, Joseph C; Ginsberg, Mark H.

In: Blood, Vol. 78, No. 2, 15.07.1991, p. 369-376.

Research output: Contribution to journalArticle

Frojmovic, Mony M. ; O'Toole, Timothy E. ; Plow, Edward F. ; Loftus, Joseph C ; Ginsberg, Mark H. / Platelet glycoprotein IIb-IIIaIIbβ3 integrin) confers fibrinogen- and activation-dependent aggregation on heterologous cells. In: Blood. 1991 ; Vol. 78, No. 2. pp. 369-376.
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