TY - JOUR
T1 - Persistent infection of oligodendrocytes in Theiler's virus‐induced encephalomyelitis
AU - Rodriguez, Moses
AU - Leibowitz, Julian L.
AU - Lampert, Peter W.
PY - 1983/4
Y1 - 1983/4
N2 - Mice infected with Theiler's murine encephalomyelitis virus (TMEV) develop a chronic relapsing demyelinating myelitis. To determine the localization of viral antigen in infected cells of the spinal cord, we studied TMEV‐infected SJL/J mice using immunoelectron microscopic peroxidase‐antiperoxidase techniques. Viral antigens were expressed in the cytoplasm of neurons and astrocytes 4 and 11 days postinfection. At 28 days postinfection, macrophages astrocytes, and oligodendrocytes showed viral antigen in their cytoplasm. At 45, 83, 270, and 360 days postinfection, most infected cells were oligodendrocytes as revealed ultrastructurally by immunoperoxidase staining of prominent glial loops that connect with myelin lameliae. Some of these sheaths also showed Schmidt‐Lanterman incisures that stained for viral antigen. Virus could be recovered at low titers for the duration of the illness. The findings indicate that TMEV induces persistent infection of oligodendrocytes which could then become the target of immune‐mediated injury resulting in demyelination.
AB - Mice infected with Theiler's murine encephalomyelitis virus (TMEV) develop a chronic relapsing demyelinating myelitis. To determine the localization of viral antigen in infected cells of the spinal cord, we studied TMEV‐infected SJL/J mice using immunoelectron microscopic peroxidase‐antiperoxidase techniques. Viral antigens were expressed in the cytoplasm of neurons and astrocytes 4 and 11 days postinfection. At 28 days postinfection, macrophages astrocytes, and oligodendrocytes showed viral antigen in their cytoplasm. At 45, 83, 270, and 360 days postinfection, most infected cells were oligodendrocytes as revealed ultrastructurally by immunoperoxidase staining of prominent glial loops that connect with myelin lameliae. Some of these sheaths also showed Schmidt‐Lanterman incisures that stained for viral antigen. Virus could be recovered at low titers for the duration of the illness. The findings indicate that TMEV induces persistent infection of oligodendrocytes which could then become the target of immune‐mediated injury resulting in demyelination.
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U2 - 10.1002/ana.410130409
DO - 10.1002/ana.410130409
M3 - Article
C2 - 6340596
AN - SCOPUS:0020630814
SN - 0364-5134
VL - 13
SP - 426
EP - 433
JO - Annals of neurology
JF - Annals of neurology
IS - 4
ER -