ZO-1 recruitment to α-catenin -a novel mechanism for coupling the assembly of tight junctions to adherens junctions

Jessica Maiers, Xiao Peng, Alan S. Fanning, Kris A. DeMali

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

The formation of a barrier between epithelial cells is a fundamental determinant of cellular homeostasis, protecting underlying cells against pathogens, dehydration and damage. Assembly of the tight junction barrier is dependent upon neighboring epithelial cells binding to one another and forming adherens junctions, but the mechanism for how these processes are linked is poorly understood. Using a knockdown and substitution system, we studied whether ZO-1 binding to α-catenin is required for coupling tight junction assembly to the formation of adherens junctions. We found that preventing ZO-1 binding to α-catenin did not appear to affect adherens junctions. Rather the assembly and maintenance of the epithelial barrier were disrupted. This disruption was accompanied by alterations in the mobility of ZO-1 and the organization of the actin cytoskeleton. Thus, our study identifies a-catenin binding to ZO-1 as a new mechanism for coupling the assembly of the epithelial barrier to cell-to-cell adhesion.

Original languageEnglish (US)
Pages (from-to)3904-3915
Number of pages12
JournalJournal of Cell Science
Volume126
Issue number17
DOIs
StatePublished - Sep 5 2013
Externally publishedYes

Fingerprint

Adherens Junctions
Catenins
Tight Junctions
Epithelial Cells
Actin Cytoskeleton
Dehydration
Cell Adhesion
Homeostasis
Maintenance

Keywords

  • α-Catenin
  • Adherens junction
  • Tight junction
  • ZO-1

ASJC Scopus subject areas

  • Cell Biology

Cite this

ZO-1 recruitment to α-catenin -a novel mechanism for coupling the assembly of tight junctions to adherens junctions. / Maiers, Jessica; Peng, Xiao; Fanning, Alan S.; DeMali, Kris A.

In: Journal of Cell Science, Vol. 126, No. 17, 05.09.2013, p. 3904-3915.

Research output: Contribution to journalArticle

@article{ca7a0f56379f4b6e960bb0d2424a9bc2,
title = "ZO-1 recruitment to α-catenin -a novel mechanism for coupling the assembly of tight junctions to adherens junctions",
abstract = "The formation of a barrier between epithelial cells is a fundamental determinant of cellular homeostasis, protecting underlying cells against pathogens, dehydration and damage. Assembly of the tight junction barrier is dependent upon neighboring epithelial cells binding to one another and forming adherens junctions, but the mechanism for how these processes are linked is poorly understood. Using a knockdown and substitution system, we studied whether ZO-1 binding to α-catenin is required for coupling tight junction assembly to the formation of adherens junctions. We found that preventing ZO-1 binding to α-catenin did not appear to affect adherens junctions. Rather the assembly and maintenance of the epithelial barrier were disrupted. This disruption was accompanied by alterations in the mobility of ZO-1 and the organization of the actin cytoskeleton. Thus, our study identifies a-catenin binding to ZO-1 as a new mechanism for coupling the assembly of the epithelial barrier to cell-to-cell adhesion.",
keywords = "α-Catenin, Adherens junction, Tight junction, ZO-1",
author = "Jessica Maiers and Xiao Peng and Fanning, {Alan S.} and DeMali, {Kris A.}",
year = "2013",
month = "9",
day = "5",
doi = "10.1242/jcs.126565",
language = "English (US)",
volume = "126",
pages = "3904--3915",
journal = "Journal of Cell Science",
issn = "0021-9533",
publisher = "Company of Biologists Ltd",
number = "17",

}

TY - JOUR

T1 - ZO-1 recruitment to α-catenin -a novel mechanism for coupling the assembly of tight junctions to adherens junctions

AU - Maiers, Jessica

AU - Peng, Xiao

AU - Fanning, Alan S.

AU - DeMali, Kris A.

PY - 2013/9/5

Y1 - 2013/9/5

N2 - The formation of a barrier between epithelial cells is a fundamental determinant of cellular homeostasis, protecting underlying cells against pathogens, dehydration and damage. Assembly of the tight junction barrier is dependent upon neighboring epithelial cells binding to one another and forming adherens junctions, but the mechanism for how these processes are linked is poorly understood. Using a knockdown and substitution system, we studied whether ZO-1 binding to α-catenin is required for coupling tight junction assembly to the formation of adherens junctions. We found that preventing ZO-1 binding to α-catenin did not appear to affect adherens junctions. Rather the assembly and maintenance of the epithelial barrier were disrupted. This disruption was accompanied by alterations in the mobility of ZO-1 and the organization of the actin cytoskeleton. Thus, our study identifies a-catenin binding to ZO-1 as a new mechanism for coupling the assembly of the epithelial barrier to cell-to-cell adhesion.

AB - The formation of a barrier between epithelial cells is a fundamental determinant of cellular homeostasis, protecting underlying cells against pathogens, dehydration and damage. Assembly of the tight junction barrier is dependent upon neighboring epithelial cells binding to one another and forming adherens junctions, but the mechanism for how these processes are linked is poorly understood. Using a knockdown and substitution system, we studied whether ZO-1 binding to α-catenin is required for coupling tight junction assembly to the formation of adherens junctions. We found that preventing ZO-1 binding to α-catenin did not appear to affect adherens junctions. Rather the assembly and maintenance of the epithelial barrier were disrupted. This disruption was accompanied by alterations in the mobility of ZO-1 and the organization of the actin cytoskeleton. Thus, our study identifies a-catenin binding to ZO-1 as a new mechanism for coupling the assembly of the epithelial barrier to cell-to-cell adhesion.

KW - α-Catenin

KW - Adherens junction

KW - Tight junction

KW - ZO-1

UR - http://www.scopus.com/inward/record.url?scp=84883301688&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84883301688&partnerID=8YFLogxK

U2 - 10.1242/jcs.126565

DO - 10.1242/jcs.126565

M3 - Article

C2 - 23813953

AN - SCOPUS:84883301688

VL - 126

SP - 3904

EP - 3915

JO - Journal of Cell Science

JF - Journal of Cell Science

SN - 0021-9533

IS - 17

ER -