Zinc modulates the structure and binding of the DNA binding domain of the 1α,25-dihydroxyvitamin D3 receptor to specific vitamin D response element DNA. To determine whether zinc alters 1α,25-dihydroxyvitamin D3-regulated genes in cells, we permanently transfected rat osteoblasts with two vitamin D-dependent promoter-reporter systems and examined their responses to 1α,25-dihydroxyvitamin D3 in the presence of increasing amounts of extracellular zinc. When extracellular zinc concentrations were increased in the presence of 1α,25-dihydroxyvitamin D3, there was an increase in the activity of 1α,25-dihydroxyvitamin D3-dependent promoters with increasing concentrations of zinc. The effect was specific for zinc since metals such as copper failed to increase the activity of 1α,25-dihydroxyvitamin D3-dependent promoters. The concentration of the vitamin D receptor within the cell and the affinity of 1α,25-dihydroxyvitamin D3 for its receptor remained unchanged with added zinc. Our results show that zinc increases the activity of 1α,25-dihydroxyvitamin D3-dependent promoters in osteoblasts. (C) 2000 Academic Press.
|Original language||English (US)|
|Number of pages||7|
|Journal||Biochemical and Biophysical Research Communications|
|State||Published - Apr 29 2000|
ASJC Scopus subject areas
- Molecular Biology