Zinc increases the activity of vitamin D-dependent promoters in osteoblasts

Ward Lutz, Mary F. Burritt, David E. Nixon, Pai Chih Kao, Rajiv Kumar

Research output: Contribution to journalArticle

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Abstract

Zinc modulates the structure and binding of the DNA binding domain of the 1α,25-dihydroxyvitamin D3 receptor to specific vitamin D response element DNA. To determine whether zinc alters 1α,25-dihydroxyvitamin D3-regulated genes in cells, we permanently transfected rat osteoblasts with two vitamin D-dependent promoter-reporter systems and examined their responses to 1α,25-dihydroxyvitamin D3 in the presence of increasing amounts of extracellular zinc. When extracellular zinc concentrations were increased in the presence of 1α,25-dihydroxyvitamin D3, there was an increase in the activity of 1α,25-dihydroxyvitamin D3-dependent promoters with increasing concentrations of zinc. The effect was specific for zinc since metals such as copper failed to increase the activity of 1α,25-dihydroxyvitamin D3-dependent promoters. The concentration of the vitamin D receptor within the cell and the affinity of 1α,25-dihydroxyvitamin D3 for its receptor remained unchanged with added zinc. Our results show that zinc increases the activity of 1α,25-dihydroxyvitamin D3-dependent promoters in osteoblasts. (C) 2000 Academic Press.

Original languageEnglish (US)
Pages (from-to)1-7
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume271
Issue number1
DOIs
StatePublished - Apr 29 2000

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Osteoblasts
Vitamin D
Zinc
Calcitriol
Calcitriol Receptors
Vitamin D Response Element
DNA
Rats
Copper
Genes
Metals

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Zinc increases the activity of vitamin D-dependent promoters in osteoblasts. / Lutz, Ward; Burritt, Mary F.; Nixon, David E.; Kao, Pai Chih; Kumar, Rajiv.

In: Biochemical and Biophysical Research Communications, Vol. 271, No. 1, 29.04.2000, p. 1-7.

Research output: Contribution to journalArticle

Lutz, Ward ; Burritt, Mary F. ; Nixon, David E. ; Kao, Pai Chih ; Kumar, Rajiv. / Zinc increases the activity of vitamin D-dependent promoters in osteoblasts. In: Biochemical and Biophysical Research Communications. 2000 ; Vol. 271, No. 1. pp. 1-7.
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