Zinc increases the activity of vitamin D-dependent promoters in osteoblasts

Ward Lutz, Mary F. Burritt, David E. Nixon, Pai Chih Kao, Rajiv Kumar

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35 Scopus citations

Abstract

Zinc modulates the structure and binding of the DNA binding domain of the 1α,25-dihydroxyvitamin D3 receptor to specific vitamin D response element DNA. To determine whether zinc alters 1α,25-dihydroxyvitamin D3-regulated genes in cells, we permanently transfected rat osteoblasts with two vitamin D-dependent promoter-reporter systems and examined their responses to 1α,25-dihydroxyvitamin D3 in the presence of increasing amounts of extracellular zinc. When extracellular zinc concentrations were increased in the presence of 1α,25-dihydroxyvitamin D3, there was an increase in the activity of 1α,25-dihydroxyvitamin D3-dependent promoters with increasing concentrations of zinc. The effect was specific for zinc since metals such as copper failed to increase the activity of 1α,25-dihydroxyvitamin D3-dependent promoters. The concentration of the vitamin D receptor within the cell and the affinity of 1α,25-dihydroxyvitamin D3 for its receptor remained unchanged with added zinc. Our results show that zinc increases the activity of 1α,25-dihydroxyvitamin D3-dependent promoters in osteoblasts. (C) 2000 Academic Press.

Original languageEnglish (US)
Pages (from-to)1-7
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume271
Issue number1
DOIs
StatePublished - Apr 29 2000

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ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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