TY - JOUR
T1 - Zinc increases the activity of vitamin D-dependent promoters in osteoblasts
AU - Lutz, Ward
AU - Burritt, Mary F.
AU - Nixon, David E.
AU - Kao, Pai Chih
AU - Kumar, Rajiv
N1 - Funding Information:
This study was supported by the National Institutes of Health Grants DK25409 and AR27037.
PY - 2000/4/29
Y1 - 2000/4/29
N2 - Zinc modulates the structure and binding of the DNA binding domain of the 1α,25-dihydroxyvitamin D3 receptor to specific vitamin D response element DNA. To determine whether zinc alters 1α,25-dihydroxyvitamin D3-regulated genes in cells, we permanently transfected rat osteoblasts with two vitamin D-dependent promoter-reporter systems and examined their responses to 1α,25-dihydroxyvitamin D3 in the presence of increasing amounts of extracellular zinc. When extracellular zinc concentrations were increased in the presence of 1α,25-dihydroxyvitamin D3, there was an increase in the activity of 1α,25-dihydroxyvitamin D3-dependent promoters with increasing concentrations of zinc. The effect was specific for zinc since metals such as copper failed to increase the activity of 1α,25-dihydroxyvitamin D3-dependent promoters. The concentration of the vitamin D receptor within the cell and the affinity of 1α,25-dihydroxyvitamin D3 for its receptor remained unchanged with added zinc. Our results show that zinc increases the activity of 1α,25-dihydroxyvitamin D3-dependent promoters in osteoblasts. (C) 2000 Academic Press.
AB - Zinc modulates the structure and binding of the DNA binding domain of the 1α,25-dihydroxyvitamin D3 receptor to specific vitamin D response element DNA. To determine whether zinc alters 1α,25-dihydroxyvitamin D3-regulated genes in cells, we permanently transfected rat osteoblasts with two vitamin D-dependent promoter-reporter systems and examined their responses to 1α,25-dihydroxyvitamin D3 in the presence of increasing amounts of extracellular zinc. When extracellular zinc concentrations were increased in the presence of 1α,25-dihydroxyvitamin D3, there was an increase in the activity of 1α,25-dihydroxyvitamin D3-dependent promoters with increasing concentrations of zinc. The effect was specific for zinc since metals such as copper failed to increase the activity of 1α,25-dihydroxyvitamin D3-dependent promoters. The concentration of the vitamin D receptor within the cell and the affinity of 1α,25-dihydroxyvitamin D3 for its receptor remained unchanged with added zinc. Our results show that zinc increases the activity of 1α,25-dihydroxyvitamin D3-dependent promoters in osteoblasts. (C) 2000 Academic Press.
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U2 - 10.1006/bbrc.2000.2570
DO - 10.1006/bbrc.2000.2570
M3 - Article
C2 - 10777672
AN - SCOPUS:0034728763
SN - 0006-291X
VL - 271
SP - 1
EP - 7
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 1
ER -