Zinc finger-based knockout punches for zebrafish genes

Research output: Contribution to journalShort survey

61 Scopus citations

Abstract

The ability to manipulate the genome is critical to develop and test hypotheses based on genetics. Knockdown strategies focused on RNAi and/or morpholinos are excellent genetic tools, but they come with substantial technical limitations. A new gene targeting approach employing synthetic zinc finger nuclease (ZFN) technology is a powerful and complementary approach to directly modify genetic loci for many diverse applications, notably enhancing Danio rerio (the zebrafish) as an experimental organism for understanding human disease. This ZFN-based technology to generate targeted knockouts in this aquatic animal opens the door to an array of new biological models of human disease and genetic testing.

Original languageEnglish (US)
Pages (from-to)121-123
Number of pages3
JournalZebrafish
Volume5
Issue number2
DOIs
StatePublished - Jun 1 2008

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ASJC Scopus subject areas

  • Animal Science and Zoology
  • Developmental Biology

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