Zinc finger-based knockout punches for zebrafish genes

Research output: Contribution to journalArticle

61 Citations (Scopus)

Abstract

The ability to manipulate the genome is critical to develop and test hypotheses based on genetics. Knockdown strategies focused on RNAi and/or morpholinos are excellent genetic tools, but they come with substantial technical limitations. A new gene targeting approach employing synthetic zinc finger nuclease (ZFN) technology is a powerful and complementary approach to directly modify genetic loci for many diverse applications, notably enhancing Danio rerio (the zebrafish) as an experimental organism for understanding human disease. This ZFN-based technology to generate targeted knockouts in this aquatic animal opens the door to an array of new biological models of human disease and genetic testing.

Original languageEnglish (US)
Pages (from-to)121-123
Number of pages3
JournalZebrafish
Volume5
Issue number2
DOIs
StatePublished - Jun 1 2008

Fingerprint

zinc finger motif
Zinc Fingers
Zebrafish
Danio rerio
nucleases
zinc
Technology
human diseases
Morpholinos
Biological Models
Genetic Loci
Gene Targeting
gene
Medical Genetics
Genetic Testing
RNA Interference
Genes
genes
gene targeting
Genome

ASJC Scopus subject areas

  • Developmental Biology
  • Molecular Biology
  • Aquatic Science

Cite this

Zinc finger-based knockout punches for zebrafish genes. / Ekker, Stephen C.

In: Zebrafish, Vol. 5, No. 2, 01.06.2008, p. 121-123.

Research output: Contribution to journalArticle

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