Xenotropic virus-related restriction patterns of non-H-2 histocompatibility mutant mice strains

P. J. Wettstein, R. W. Melvold

Research output: Contribution to journalArticle

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Abstract

The relationship between alteration in the number of xenotropic virus-related sequences and non-H-2 histocompatibility (H) mutations in mice was investigated. Mutant classifications included gain, loss, and loss-gain mutations. Genomic DNA from a panel of non-H-2 H mutant strains on the C57BL/6 and BALB/c backgrounds was digested with a set of restriction enzymes with varying numbers of sites within endogenous xenotropic-related sequences. The digested DNA was then resolved on agarose gels. Southern blots of digested DNA were hybridized with the pX(env) probe specific for the env sequence of xenotropic viral sequences. The number of hybridizing bands varied from 7 to 19, depending on the restriction enzyme and inbred background. Most mutant strains were identical in their restriction patterns to the respective background strains. However, two B6 mutant strains, KH84 and HZ54, differed from C57BL/6 at a single band which appeared to be inherited from BALB/c in the derivation of the two congenic strains. The HZ43 strain lacked a male-specific band shared by both C57BL/6 and BALB/c; this loss was evidently independent of the original mutation which was observed to be autosomal. However, the KH148B and KH84 strains on the C57BL/6 background lacked single B6 bands. Both mutants were classified as gain mutants. An examination of previous reciprocal graft rejection patterns and retrovirus linkage to non-H-2 H loci indicated a strong inverse relationship between a linked retroviral sequence and presentation of a non-H-2 H antigen. This inverse correlation is consistent with reports of gene inactivation following retroviral insertion.

Original languageEnglish (US)
Pages (from-to)156-163
Number of pages8
JournalImmunogenetics
Volume23
Issue number3
StatePublished - 1986
Externally publishedYes

Fingerprint

Mutant Strains Mice
Histocompatibility
Viruses
Mutation
DNA
H-2 Antigens
Graft Rejection
Gene Silencing
Retroviridae
Enzymes
Southern Blotting
Sepharose
Gels

ASJC Scopus subject areas

  • Genetics
  • Immunology

Cite this

Xenotropic virus-related restriction patterns of non-H-2 histocompatibility mutant mice strains. / Wettstein, P. J.; Melvold, R. W.

In: Immunogenetics, Vol. 23, No. 3, 1986, p. 156-163.

Research output: Contribution to journalArticle

Wettstein, P. J. ; Melvold, R. W. / Xenotropic virus-related restriction patterns of non-H-2 histocompatibility mutant mice strains. In: Immunogenetics. 1986 ; Vol. 23, No. 3. pp. 156-163.
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