@article{826eca06a83d4497953bf7e4edeeb7d2,
title = "Whole genome sequencing of cyanobacterium Nostoc sp. CCCryo 231-06 using microfluidic single cell technology",
abstract = "The Nostoc sp. strain CCCryo 231-06 is a cyanobacterial strain capable of surviving under extreme conditions and thus is of great interest for the astrobiology community. The knowledge of its complete genome sequence would serve as a guide for further studies. However, a major concern has been placed on the effects of contamination on the quality of sequencing data without a reference genome. Here, we report the use of microfluidic technology combined with single cell sequencing and de novo assembly to minimize the contamination and recover the complete genome of the Nostoc strain CCCryo 231-06 with high quality. 100% of the whole genome was recovered with all contaminants removed and a strongly supported phylogenetic tree. The data reported can be useful for comparative genomics for phylogenetic and taxonomic studies. The method used in this work can be applied to studies that require high-quality assemblies of genomes of unknown microorganisms.",
keywords = "Astrobiology, Microbial genomics, Microbiology, Space sciences",
author = "Yuguang Liu and Patricio Jeraldo and William Herbert and Samantha McDonough and Bruce Eckloff and Dirk Schulze-Makuch and {de Vera}, {Jean Pierre} and Charles Cockell and Thomas Leya and Mickael Baqu{\'e} and Jin Jen and Marina Walther-Antonio",
note = "Funding Information: This research was funded by the following sources. Marina Walther-Antonio acknowledges The Ivan Bowen Family Foundation and CTSA Grant Number KL2 TR002379 from the National Center for Advancing Translational Sciences (NCATS). Its contents are solely the responsibility of the authors and do not necessarily represent the official views of the NIH. Dirk Schulze-Makuch and Jean-Pierre de Vera acknowledge support from ERC Advanced Grant “HOME” (# 339231 ), and in addition, Jean-Pierre de Vera acknowledges support from ESA for the BIOMEX project ( ESA-ILSRA 2009-0834 ) and DLR for the DLR-FuW-Project BIOMEX ( 2474128 ). Charles S. Cockell was supported by the Science and Technology Facilities Council (STFC) , Grant No. ST/M001261/1 . Mickael Baqu{\'e} acknowledges the support of the Deutsche Forschungsgemeinschaft (DFG – German Research Foundation) for the project “Raman Biosignatures for Astrobiology Research” (RaBioFAM; project number: 426601242 ). In addition, we thank the Microbiome Program and the Center for Individualized Medicine, Mayo Clinic for their support, and Dr. Alexander Revzin at Mayo Clinic for granting us the access to his microfabrication facilities. Funding Information: This research was funded by the following sources. Marina Walther-Antonio acknowledges The Ivan Bowen Family Foundation and CTSA Grant Number KL2 TR002379 from the National Center for Advancing Translational Sciences (NCATS). Its contents are solely the responsibility of the authors and do not necessarily represent the official views of the NIH. Dirk Schulze-Makuch and Jean-Pierre de Vera acknowledge support from ERC Advanced Grant “HOME” (# 339231), and in addition, Jean-Pierre de Vera acknowledges support from ESA for the BIOMEX project (ESA-ILSRA 2009-0834) and DLR for the DLR-FuW-Project BIOMEX (2474128). Charles S. Cockell was supported by the Science and Technology Facilities Council (STFC), Grant No. ST/M001261/1. Mickael Baqu{\'e} acknowledges the support of the Deutsche Forschungsgemeinschaft (DFG – German Research Foundation) for the project “Raman Biosignatures for Astrobiology Research” (RaBioFAM; project number: 426601242). In addition, we thank the Microbiome Program and the Center for Individualized Medicine, Mayo Clinic for their support, and Dr. Alexander Revzin at Mayo Clinic for granting us the access to his microfabrication facilities. Experimental methodology: Y.L. and W.M.A.; Bioinformatic methodology: P.J.; Formal analysis: P.J. Y.L. and W.H.; Library optimization, preparation, and sequencing: S.M.; Funding acquisition and project supervision: D.S.M. J.P.d.V. C.C. T.L. M.B. and M.W.A.; NGS optimization and sequencing supervision, B.E. and J.J.; Writing–original draft, Y.L. and P.J.; Writing–review & editing, Y.L. W.H. D.S.M. J.P.d.V. C.C. T.L. M.B. and M.W.A. The authors declare no competing interests. Publisher Copyright: {\textcopyright} 2022 The Author(s)",
year = "2022",
month = may,
day = "20",
doi = "10.1016/j.isci.2022.104291",
language = "English (US)",
volume = "25",
journal = "iScience",
issn = "2589-0042",
publisher = "Elsevier Inc.",
number = "5",
}