Whole cell electrophysiology of primary cultured murine enterochromaffin cells

Katilyn Knutson, Peter R. Strege, Joyce Li, Andrew B. Leiter, Gianrico Farrugia, Arthur Beyder

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Enterochromaffin (EC) cells in the gastrointestinal (GI) epithelium constitute the largest subpopulation of enteroendocrine cells. As specialized sensory cells, EC cells sense luminal stimuli and convert them into serotonin (5-hyroxytryptamine, 5-HT) release events. However, the electrophysiology of these cells is poorly understood because they are difficult to culture and to identify. The method presented in this paper outlines primary EC cell cultures optimized for single cell electrophysiology. This protocol utilizes a transgenic cyan fluorescent protein (CFP) reporter to identify mouse EC cells in mixed primary cultures, advancing the approach to obtaining high-quality recordings of whole cell electrophysiology in voltage-and current-clamp modes.

Original languageEnglish (US)
Article numbere58112
JournalJournal of Visualized Experiments
Volume2018
Issue number139
DOIs
StatePublished - Sep 26 2018

Fingerprint

Enterochromaffin Cells
Electrophysiology
Clamping devices
Enteroendocrine Cells
Cell culture
Primary Cell Culture
Serotonin
Patch-Clamp Techniques
Proteins
Epithelium
Electric potential

Keywords

  • Biology
  • Current clamp
  • Enterochromaffin cell
  • Epithelium
  • Gastrointestinal
  • Issue 139
  • Primary culture
  • Voltage clamp
  • Whole cell electrophysiology

ASJC Scopus subject areas

  • Neuroscience(all)
  • Chemical Engineering(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

Cite this

Whole cell electrophysiology of primary cultured murine enterochromaffin cells. / Knutson, Katilyn; Strege, Peter R.; Li, Joyce; Leiter, Andrew B.; Farrugia, Gianrico; Beyder, Arthur.

In: Journal of Visualized Experiments, Vol. 2018, No. 139, e58112, 26.09.2018.

Research output: Contribution to journalArticle

Knutson, Katilyn ; Strege, Peter R. ; Li, Joyce ; Leiter, Andrew B. ; Farrugia, Gianrico ; Beyder, Arthur. / Whole cell electrophysiology of primary cultured murine enterochromaffin cells. In: Journal of Visualized Experiments. 2018 ; Vol. 2018, No. 139.
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