Whipple's arthritis: Direct detection of Tropheryma whippelii in synovial fluid and tissue

J. Desmond O'Duffy, W. Leroy Griffing, Chin Yang Li, Manal F. Abdelmalek, David H. Persing

Research output: Contribution to journalArticle

78 Citations (Scopus)

Abstract

We describe 2 patients presenting with polyarthritis in whom the synovial fluid (1 patient) or synovial tissue (1 patient) was positive for Tropheryma whippelii, the Whipple's disease-associated bacillus, when examined by polymerase chain reaction (PCR) and DNA sequencing. Histopathologic findings were consistent with articular Whipple's disease in the synovial fluid of 1 patient and the synovial tissue of the other. In both patients, bowel mucosal specimens were negative for Whipple's disease features by histologic and PCR methods. One patient was positive for T whippelii in the peripheral blood. Control synovial fluid specimens from 40 patients with other arthritides, including Lyme arthritis, were negative. Sequencing of a 284-basepair region of the 16S ribosomal RNA gene confirmed that the sequence is closely related to the known T whippelii sequence. Both patients responded to treatment with antibiotics.

Original languageEnglish (US)
Pages (from-to)812-817
Number of pages6
JournalArthritis and Rheumatism
Volume42
Issue number4
DOIs
StatePublished - Apr 1999

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Tropheryma
Synovial Fluid
Arthritis
Whipple Disease
16S Ribosomal RNA
Polymerase Chain Reaction
Lyme Disease
DNA Sequence Analysis
rRNA Genes
Bacillus
Joints
Anti-Bacterial Agents

ASJC Scopus subject areas

  • Immunology
  • Rheumatology

Cite this

Whipple's arthritis : Direct detection of Tropheryma whippelii in synovial fluid and tissue. / O'Duffy, J. Desmond; Griffing, W. Leroy; Li, Chin Yang; Abdelmalek, Manal F.; Persing, David H.

In: Arthritis and Rheumatism, Vol. 42, No. 4, 04.1999, p. 812-817.

Research output: Contribution to journalArticle

O'Duffy, J. Desmond ; Griffing, W. Leroy ; Li, Chin Yang ; Abdelmalek, Manal F. ; Persing, David H. / Whipple's arthritis : Direct detection of Tropheryma whippelii in synovial fluid and tissue. In: Arthritis and Rheumatism. 1999 ; Vol. 42, No. 4. pp. 812-817.
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