Waldenström macroglobulinemia neoplastic cells lack immunoglobulin heavy chain locus translocations but have frequent 6q deletions

Roelandt F J Schop, W. Michael Kuehl, Scott A. Van Wier, Gregory J. Ahmann, Tammy Price-Troska, Richard J. Bailey, Syed M. Jalal, Ying Qi, Robert A. Kyle, Philip R. Greipp, Rafael Fonseca

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Abstract

Lymphoplasmacytic lymphoma (LPL) is characterized by t(9;14)(p13;q32) in 50% of patients who lack paraproteinemia. Waldenström macroglobulinemia (WM), which has an immunoglobulin M (IgM) paraproteinemia, is classified as an LPL. Rare reports have suggested that WM sometimes is associated with 14q23 translocations, deletions of 6q, and t(11;18)(q21; q21). We tested for these abnormalities in the clonal cells of WM patients. We selected patients with clinicopathologic diagnosis of WM (all had IgM levels greater than 1.5 g/dL). Southern blot assay was used to detect legitimate and illegitimate IgH switch rearrangements. In addition to conventional cytogenetic (CC) and multicolor metaphase fluorescence in situ hybridization (M-FISH) analyses, we used interphase FISH to screen for t(9;14)(p13; q32) and other IgH translocations, t(11; 18)(q21;q21), and 6q21 deletions. Genomic stability was also assessed using chromosome enumeration probes for chromosomes 7, 9, 11, 12, 15, and 17 in 15 patients. There was no evidence of either legitimate or illegitimate IgH rearrangements by Southern blot assay (n = 12). CC (n = 37), M-FISH (n = 5), and interphase FISH (n = 42) failed to identify IgH or t(11;18) translocations. Although tumor cells from most patients were diploid for the chromosomes studied, deletions of 6q21 were observed in 42% of patients. In contrast to LPL tumors that are not associated with paraproteinemia and that have frequent t(9;14)(p13;q32) translocations, IgH translocations are not found in WM, a form of LPL tumor distinguished by IgM paraproteinemia. However, WM tumor cells, which appear to be diploid or near diploid, often have deletions of 6q21.

Original languageEnglish (US)
Pages (from-to)2996-3001
Number of pages6
JournalBlood
Volume100
Issue number8
DOIs
StatePublished - Oct 15 2002

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Waldenstrom Macroglobulinemia
Immunoglobulin Heavy Chains
Tumors
Paraproteinemias
Chromosomes
Immunoglobulin M
Lymphoma
Diploidy
Assays
Fluorescence
Interphase
Cells
Metaphase
Southern Blotting
Fluorescence In Situ Hybridization
Cytogenetics
Neoplasms
Chromosome Deletion
Chromosomes, Human, Pair 9
Chromosomes, Human, Pair 7

ASJC Scopus subject areas

  • Hematology

Cite this

Schop, R. F. J., Michael Kuehl, W., Van Wier, S. A., Ahmann, G. J., Price-Troska, T., Bailey, R. J., ... Fonseca, R. (2002). Waldenström macroglobulinemia neoplastic cells lack immunoglobulin heavy chain locus translocations but have frequent 6q deletions. Blood, 100(8), 2996-3001. https://doi.org/10.1182/blood.V100.8.2996

Waldenström macroglobulinemia neoplastic cells lack immunoglobulin heavy chain locus translocations but have frequent 6q deletions. / Schop, Roelandt F J; Michael Kuehl, W.; Van Wier, Scott A.; Ahmann, Gregory J.; Price-Troska, Tammy; Bailey, Richard J.; Jalal, Syed M.; Qi, Ying; Kyle, Robert A.; Greipp, Philip R.; Fonseca, Rafael.

In: Blood, Vol. 100, No. 8, 15.10.2002, p. 2996-3001.

Research output: Contribution to journalArticle

Schop, RFJ, Michael Kuehl, W, Van Wier, SA, Ahmann, GJ, Price-Troska, T, Bailey, RJ, Jalal, SM, Qi, Y, Kyle, RA, Greipp, PR & Fonseca, R 2002, 'Waldenström macroglobulinemia neoplastic cells lack immunoglobulin heavy chain locus translocations but have frequent 6q deletions', Blood, vol. 100, no. 8, pp. 2996-3001. https://doi.org/10.1182/blood.V100.8.2996
Schop RFJ, Michael Kuehl W, Van Wier SA, Ahmann GJ, Price-Troska T, Bailey RJ et al. Waldenström macroglobulinemia neoplastic cells lack immunoglobulin heavy chain locus translocations but have frequent 6q deletions. Blood. 2002 Oct 15;100(8):2996-3001. https://doi.org/10.1182/blood.V100.8.2996
Schop, Roelandt F J ; Michael Kuehl, W. ; Van Wier, Scott A. ; Ahmann, Gregory J. ; Price-Troska, Tammy ; Bailey, Richard J. ; Jalal, Syed M. ; Qi, Ying ; Kyle, Robert A. ; Greipp, Philip R. ; Fonseca, Rafael. / Waldenström macroglobulinemia neoplastic cells lack immunoglobulin heavy chain locus translocations but have frequent 6q deletions. In: Blood. 2002 ; Vol. 100, No. 8. pp. 2996-3001.
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abstract = "Lymphoplasmacytic lymphoma (LPL) is characterized by t(9;14)(p13;q32) in 50{\%} of patients who lack paraproteinemia. Waldenstr{\"o}m macroglobulinemia (WM), which has an immunoglobulin M (IgM) paraproteinemia, is classified as an LPL. Rare reports have suggested that WM sometimes is associated with 14q23 translocations, deletions of 6q, and t(11;18)(q21; q21). We tested for these abnormalities in the clonal cells of WM patients. We selected patients with clinicopathologic diagnosis of WM (all had IgM levels greater than 1.5 g/dL). Southern blot assay was used to detect legitimate and illegitimate IgH switch rearrangements. In addition to conventional cytogenetic (CC) and multicolor metaphase fluorescence in situ hybridization (M-FISH) analyses, we used interphase FISH to screen for t(9;14)(p13; q32) and other IgH translocations, t(11; 18)(q21;q21), and 6q21 deletions. Genomic stability was also assessed using chromosome enumeration probes for chromosomes 7, 9, 11, 12, 15, and 17 in 15 patients. There was no evidence of either legitimate or illegitimate IgH rearrangements by Southern blot assay (n = 12). CC (n = 37), M-FISH (n = 5), and interphase FISH (n = 42) failed to identify IgH or t(11;18) translocations. Although tumor cells from most patients were diploid for the chromosomes studied, deletions of 6q21 were observed in 42{\%} of patients. In contrast to LPL tumors that are not associated with paraproteinemia and that have frequent t(9;14)(p13;q32) translocations, IgH translocations are not found in WM, a form of LPL tumor distinguished by IgM paraproteinemia. However, WM tumor cells, which appear to be diploid or near diploid, often have deletions of 6q21.",
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AU - Ahmann, Gregory J.

AU - Price-Troska, Tammy

AU - Bailey, Richard J.

AU - Jalal, Syed M.

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AB - Lymphoplasmacytic lymphoma (LPL) is characterized by t(9;14)(p13;q32) in 50% of patients who lack paraproteinemia. Waldenström macroglobulinemia (WM), which has an immunoglobulin M (IgM) paraproteinemia, is classified as an LPL. Rare reports have suggested that WM sometimes is associated with 14q23 translocations, deletions of 6q, and t(11;18)(q21; q21). We tested for these abnormalities in the clonal cells of WM patients. We selected patients with clinicopathologic diagnosis of WM (all had IgM levels greater than 1.5 g/dL). Southern blot assay was used to detect legitimate and illegitimate IgH switch rearrangements. In addition to conventional cytogenetic (CC) and multicolor metaphase fluorescence in situ hybridization (M-FISH) analyses, we used interphase FISH to screen for t(9;14)(p13; q32) and other IgH translocations, t(11; 18)(q21;q21), and 6q21 deletions. Genomic stability was also assessed using chromosome enumeration probes for chromosomes 7, 9, 11, 12, 15, and 17 in 15 patients. There was no evidence of either legitimate or illegitimate IgH rearrangements by Southern blot assay (n = 12). CC (n = 37), M-FISH (n = 5), and interphase FISH (n = 42) failed to identify IgH or t(11;18) translocations. Although tumor cells from most patients were diploid for the chromosomes studied, deletions of 6q21 were observed in 42% of patients. In contrast to LPL tumors that are not associated with paraproteinemia and that have frequent t(9;14)(p13;q32) translocations, IgH translocations are not found in WM, a form of LPL tumor distinguished by IgM paraproteinemia. However, WM tumor cells, which appear to be diploid or near diploid, often have deletions of 6q21.

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