Vitamin E supplementation effect on human platelet function, arachidonic acid metabolism, and plasma prostacyclin levels

M. J. Stampfer, J. A. Jakubowski, Douglas Orrick Faigel, R. Vaillancourt, D. Deykin

Research output: Contribution to journalArticlepeer-review

29 Scopus citations

Abstract

A randomized, placebo-controlled double-blind trial was conducted on 20 adults to assess the effect of vitamin E (800 IU/d 727 mg/d for 5 wk) on platelet function, arachidonic acid metabolism, and prostacyclin generation. Platelet aggregation was measured in response to collagen, arachidonic acid, and adenosine diphosphate. Thromboxane B2 was assayed in serum and in the supernatant plasma after platelet aggregation. Platelets were labeled with [3H]arachidonic acid to assess production and release of cyclooxygenase products (MDA, TXB2, and HHT), a lipoxygenase product (12-HETE), and arachidonic acid in response to stimulation by thrombin or collagen. Prostacyclin was measured in plasma and in blood collected from bleeding-time incisisons by a sensitive HPLC-RIA procedure. Despite marked increases in plasma and erythrocyte vitamin E levels in the vitamin E group, there were no significant differences between the vitamin E and placebo groups in any of the variables measured.

Original languageEnglish (US)
Pages (from-to)700-706
Number of pages7
JournalAmerican Journal of Clinical Nutrition
Volume47
Issue number4
StatePublished - 1988
Externally publishedYes

ASJC Scopus subject areas

  • Food Science
  • Medicine (miscellaneous)

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