Abstract
A randomized, placebo-controlled double-blind trial was conducted on 20 adults to assess the effect of vitamin E (800 IU/d 727 mg/d for 5 wk) on platelet function, arachidonic acid metabolism, and prostacyclin generation. Platelet aggregation was measured in response to collagen, arachidonic acid, and adenosine diphosphate. Thromboxane B2 was assayed in serum and in the supernatant plasma after platelet aggregation. Platelets were labeled with [3H]arachidonic acid to assess production and release of cyclooxygenase products (MDA, TXB2, and HHT), a lipoxygenase product (12-HETE), and arachidonic acid in response to stimulation by thrombin or collagen. Prostacyclin was measured in plasma and in blood collected from bleeding-time incisisons by a sensitive HPLC-RIA procedure. Despite marked increases in plasma and erythrocyte vitamin E levels in the vitamin E group, there were no significant differences between the vitamin E and placebo groups in any of the variables measured.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 700-706 |
| Number of pages | 7 |
| Journal | American Journal of Clinical Nutrition |
| Volume | 47 |
| Issue number | 4 |
| State | Published - 1988 |
| Externally published | Yes |
Fingerprint
ASJC Scopus subject areas
- Food Science
- Medicine (miscellaneous)
Cite this
Vitamin E supplementation effect on human platelet function, arachidonic acid metabolism, and plasma prostacyclin levels. / Stampfer, M. J.; Jakubowski, J. A.; Faigel, Douglas Orrick; Vaillancourt, R.; Deykin, D.
In: American Journal of Clinical Nutrition, Vol. 47, No. 4, 1988, p. 700-706.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Vitamin E supplementation effect on human platelet function, arachidonic acid metabolism, and plasma prostacyclin levels
AU - Stampfer, M. J.
AU - Jakubowski, J. A.
AU - Faigel, Douglas Orrick
AU - Vaillancourt, R.
AU - Deykin, D.
PY - 1988
Y1 - 1988
N2 - A randomized, placebo-controlled double-blind trial was conducted on 20 adults to assess the effect of vitamin E (800 IU/d 727 mg/d for 5 wk) on platelet function, arachidonic acid metabolism, and prostacyclin generation. Platelet aggregation was measured in response to collagen, arachidonic acid, and adenosine diphosphate. Thromboxane B2 was assayed in serum and in the supernatant plasma after platelet aggregation. Platelets were labeled with [3H]arachidonic acid to assess production and release of cyclooxygenase products (MDA, TXB2, and HHT), a lipoxygenase product (12-HETE), and arachidonic acid in response to stimulation by thrombin or collagen. Prostacyclin was measured in plasma and in blood collected from bleeding-time incisisons by a sensitive HPLC-RIA procedure. Despite marked increases in plasma and erythrocyte vitamin E levels in the vitamin E group, there were no significant differences between the vitamin E and placebo groups in any of the variables measured.
AB - A randomized, placebo-controlled double-blind trial was conducted on 20 adults to assess the effect of vitamin E (800 IU/d 727 mg/d for 5 wk) on platelet function, arachidonic acid metabolism, and prostacyclin generation. Platelet aggregation was measured in response to collagen, arachidonic acid, and adenosine diphosphate. Thromboxane B2 was assayed in serum and in the supernatant plasma after platelet aggregation. Platelets were labeled with [3H]arachidonic acid to assess production and release of cyclooxygenase products (MDA, TXB2, and HHT), a lipoxygenase product (12-HETE), and arachidonic acid in response to stimulation by thrombin or collagen. Prostacyclin was measured in plasma and in blood collected from bleeding-time incisisons by a sensitive HPLC-RIA procedure. Despite marked increases in plasma and erythrocyte vitamin E levels in the vitamin E group, there were no significant differences between the vitamin E and placebo groups in any of the variables measured.
UR - http://www.scopus.com/inward/record.url?scp=0023945965&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0023945965&partnerID=8YFLogxK
M3 - Article
C2 - 3128100
AN - SCOPUS:0023945965
VL - 47
SP - 700
EP - 706
JO - American Journal of Clinical Nutrition
JF - American Journal of Clinical Nutrition
SN - 0002-9165
IS - 4
ER -