Vitamin D deficiency is associated with an increased autoimmune response in healthy individuals and in patients with systemic lupus erythematosus

Lauren L. Ritterhouse, Sherry R. Crowe, Timothy B. Niewold, Diane L. Kamen, Susan R. Macwana, Virginia C. Roberts, Amy B. Dedeke, John B. Harley, R. Hal Scofield, Joel M. Guthridge, Judith A. James

Research output: Contribution to journalArticle

146 Citations (Scopus)

Abstract

Objectives: Vitamin D deficiency is widespread and has been associated with many chronic diseases, including autoimmune disorders. A study was undertaken to explore the impact of low vitamin D levels on autoantibody production in healthy individuals, as well as B cell hyperactivity and interferon α (IFNα) activity in patients with systemic lupus erythematosus (SLE). Methods: Serum samples from 32 European American female patients with SLE and 32 matched controls were tested for 25-hydroxyvitamin D (25(OH)D) levels, lupus-associated autoantibodies and serum IFNα activity. Isolated peripheral blood mononuclear cells were tested for intracellular phospho-ERK 1/2 as a measure of B cell activation status. Results: Vitamin D deficiency (25(OH)D <20 ng/ml) was significantly more frequent among patients with SLE (n=32, 69%) and antinuclear antibody (ANA)-positive controls (n=14, 71%) compared with ANA-negative controls (n=18, 22%) (OR 7.7, 95% CI 2.0 to 29.4, p=0.003 and OR 8.8, 95% CI 1.8 to 43.6, p=0.011, respectively). Patients with high B cell activation had lower mean (SD) 25(OH)D levels than patients with low B cell activation (17.2 (5.1) vs 24.2 (3.9) ng/ml; p=0.009). Patients with vitamin D deficiency also had higher mean (SD) serum IFNα activity than patients without vitamin D deficiency (3.5 (6.6) vs 0.3 (0.3); p=0.02). Conclusions: The observation that ANA-positive healthy controls are significantly more likely to be deficient in vitamin D than ANA-negative healthy controls, together with the finding that vitamin D deficiency is associated with certain immune abnormalities in SLE, suggests that vitamin D plays an important role in autoantibody production and SLE pathogenesis.

Original languageEnglish (US)
Pages (from-to)1569-1574
Number of pages6
JournalAnnals of the Rheumatic Diseases
Volume70
Issue number9
DOIs
StatePublished - Sep 2011
Externally publishedYes

Fingerprint

Vitamin D Deficiency
Autoimmunity
Vitamin D
Systemic Lupus Erythematosus
Antinuclear Antibodies
B-Lymphocytes
Autoantibodies
Interferons
Cells
Chemical activation
Serum
Blood Cells
Chronic Disease
Blood

ASJC Scopus subject areas

  • Rheumatology
  • Immunology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Allergy

Cite this

Vitamin D deficiency is associated with an increased autoimmune response in healthy individuals and in patients with systemic lupus erythematosus. / Ritterhouse, Lauren L.; Crowe, Sherry R.; Niewold, Timothy B.; Kamen, Diane L.; Macwana, Susan R.; Roberts, Virginia C.; Dedeke, Amy B.; Harley, John B.; Scofield, R. Hal; Guthridge, Joel M.; James, Judith A.

In: Annals of the Rheumatic Diseases, Vol. 70, No. 9, 09.2011, p. 1569-1574.

Research output: Contribution to journalArticle

Ritterhouse, LL, Crowe, SR, Niewold, TB, Kamen, DL, Macwana, SR, Roberts, VC, Dedeke, AB, Harley, JB, Scofield, RH, Guthridge, JM & James, JA 2011, 'Vitamin D deficiency is associated with an increased autoimmune response in healthy individuals and in patients with systemic lupus erythematosus', Annals of the Rheumatic Diseases, vol. 70, no. 9, pp. 1569-1574. https://doi.org/10.1136/ard.2010.148494
Ritterhouse, Lauren L. ; Crowe, Sherry R. ; Niewold, Timothy B. ; Kamen, Diane L. ; Macwana, Susan R. ; Roberts, Virginia C. ; Dedeke, Amy B. ; Harley, John B. ; Scofield, R. Hal ; Guthridge, Joel M. ; James, Judith A. / Vitamin D deficiency is associated with an increased autoimmune response in healthy individuals and in patients with systemic lupus erythematosus. In: Annals of the Rheumatic Diseases. 2011 ; Vol. 70, No. 9. pp. 1569-1574.
@article{68b735864ce24a689f4b5be2e1857bef,
title = "Vitamin D deficiency is associated with an increased autoimmune response in healthy individuals and in patients with systemic lupus erythematosus",
abstract = "Objectives: Vitamin D deficiency is widespread and has been associated with many chronic diseases, including autoimmune disorders. A study was undertaken to explore the impact of low vitamin D levels on autoantibody production in healthy individuals, as well as B cell hyperactivity and interferon α (IFNα) activity in patients with systemic lupus erythematosus (SLE). Methods: Serum samples from 32 European American female patients with SLE and 32 matched controls were tested for 25-hydroxyvitamin D (25(OH)D) levels, lupus-associated autoantibodies and serum IFNα activity. Isolated peripheral blood mononuclear cells were tested for intracellular phospho-ERK 1/2 as a measure of B cell activation status. Results: Vitamin D deficiency (25(OH)D <20 ng/ml) was significantly more frequent among patients with SLE (n=32, 69{\%}) and antinuclear antibody (ANA)-positive controls (n=14, 71{\%}) compared with ANA-negative controls (n=18, 22{\%}) (OR 7.7, 95{\%} CI 2.0 to 29.4, p=0.003 and OR 8.8, 95{\%} CI 1.8 to 43.6, p=0.011, respectively). Patients with high B cell activation had lower mean (SD) 25(OH)D levels than patients with low B cell activation (17.2 (5.1) vs 24.2 (3.9) ng/ml; p=0.009). Patients with vitamin D deficiency also had higher mean (SD) serum IFNα activity than patients without vitamin D deficiency (3.5 (6.6) vs 0.3 (0.3); p=0.02). Conclusions: The observation that ANA-positive healthy controls are significantly more likely to be deficient in vitamin D than ANA-negative healthy controls, together with the finding that vitamin D deficiency is associated with certain immune abnormalities in SLE, suggests that vitamin D plays an important role in autoantibody production and SLE pathogenesis.",
author = "Ritterhouse, {Lauren L.} and Crowe, {Sherry R.} and Niewold, {Timothy B.} and Kamen, {Diane L.} and Macwana, {Susan R.} and Roberts, {Virginia C.} and Dedeke, {Amy B.} and Harley, {John B.} and Scofield, {R. Hal} and Guthridge, {Joel M.} and James, {Judith A.}",
year = "2011",
month = "9",
doi = "10.1136/ard.2010.148494",
language = "English (US)",
volume = "70",
pages = "1569--1574",
journal = "Annals of the Rheumatic Diseases",
issn = "0003-4967",
publisher = "BMJ Publishing Group",
number = "9",

}

TY - JOUR

T1 - Vitamin D deficiency is associated with an increased autoimmune response in healthy individuals and in patients with systemic lupus erythematosus

AU - Ritterhouse, Lauren L.

AU - Crowe, Sherry R.

AU - Niewold, Timothy B.

AU - Kamen, Diane L.

AU - Macwana, Susan R.

AU - Roberts, Virginia C.

AU - Dedeke, Amy B.

AU - Harley, John B.

AU - Scofield, R. Hal

AU - Guthridge, Joel M.

AU - James, Judith A.

PY - 2011/9

Y1 - 2011/9

N2 - Objectives: Vitamin D deficiency is widespread and has been associated with many chronic diseases, including autoimmune disorders. A study was undertaken to explore the impact of low vitamin D levels on autoantibody production in healthy individuals, as well as B cell hyperactivity and interferon α (IFNα) activity in patients with systemic lupus erythematosus (SLE). Methods: Serum samples from 32 European American female patients with SLE and 32 matched controls were tested for 25-hydroxyvitamin D (25(OH)D) levels, lupus-associated autoantibodies and serum IFNα activity. Isolated peripheral blood mononuclear cells were tested for intracellular phospho-ERK 1/2 as a measure of B cell activation status. Results: Vitamin D deficiency (25(OH)D <20 ng/ml) was significantly more frequent among patients with SLE (n=32, 69%) and antinuclear antibody (ANA)-positive controls (n=14, 71%) compared with ANA-negative controls (n=18, 22%) (OR 7.7, 95% CI 2.0 to 29.4, p=0.003 and OR 8.8, 95% CI 1.8 to 43.6, p=0.011, respectively). Patients with high B cell activation had lower mean (SD) 25(OH)D levels than patients with low B cell activation (17.2 (5.1) vs 24.2 (3.9) ng/ml; p=0.009). Patients with vitamin D deficiency also had higher mean (SD) serum IFNα activity than patients without vitamin D deficiency (3.5 (6.6) vs 0.3 (0.3); p=0.02). Conclusions: The observation that ANA-positive healthy controls are significantly more likely to be deficient in vitamin D than ANA-negative healthy controls, together with the finding that vitamin D deficiency is associated with certain immune abnormalities in SLE, suggests that vitamin D plays an important role in autoantibody production and SLE pathogenesis.

AB - Objectives: Vitamin D deficiency is widespread and has been associated with many chronic diseases, including autoimmune disorders. A study was undertaken to explore the impact of low vitamin D levels on autoantibody production in healthy individuals, as well as B cell hyperactivity and interferon α (IFNα) activity in patients with systemic lupus erythematosus (SLE). Methods: Serum samples from 32 European American female patients with SLE and 32 matched controls were tested for 25-hydroxyvitamin D (25(OH)D) levels, lupus-associated autoantibodies and serum IFNα activity. Isolated peripheral blood mononuclear cells were tested for intracellular phospho-ERK 1/2 as a measure of B cell activation status. Results: Vitamin D deficiency (25(OH)D <20 ng/ml) was significantly more frequent among patients with SLE (n=32, 69%) and antinuclear antibody (ANA)-positive controls (n=14, 71%) compared with ANA-negative controls (n=18, 22%) (OR 7.7, 95% CI 2.0 to 29.4, p=0.003 and OR 8.8, 95% CI 1.8 to 43.6, p=0.011, respectively). Patients with high B cell activation had lower mean (SD) 25(OH)D levels than patients with low B cell activation (17.2 (5.1) vs 24.2 (3.9) ng/ml; p=0.009). Patients with vitamin D deficiency also had higher mean (SD) serum IFNα activity than patients without vitamin D deficiency (3.5 (6.6) vs 0.3 (0.3); p=0.02). Conclusions: The observation that ANA-positive healthy controls are significantly more likely to be deficient in vitamin D than ANA-negative healthy controls, together with the finding that vitamin D deficiency is associated with certain immune abnormalities in SLE, suggests that vitamin D plays an important role in autoantibody production and SLE pathogenesis.

UR - http://www.scopus.com/inward/record.url?scp=80051475298&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=80051475298&partnerID=8YFLogxK

U2 - 10.1136/ard.2010.148494

DO - 10.1136/ard.2010.148494

M3 - Article

C2 - 21586442

AN - SCOPUS:80051475298

VL - 70

SP - 1569

EP - 1574

JO - Annals of the Rheumatic Diseases

JF - Annals of the Rheumatic Diseases

SN - 0003-4967

IS - 9

ER -