Vitamin D and mineral deficiencies increase the plasma membrane calcium pump of chicken intestine

Robert H. Wasserman, Christina A. Smith, Marie E. Brindak, Nori De Talamoni, Curtis S. Fullmer, John T. Penniston, Rajiv Kumar

Research output: Contribution to journalArticle

100 Citations (Scopus)

Abstract

The basolateral membrane of the enterocyte was previously shown to contain an adenosine triphosphate-dependent calcium pump. Using immunological procedures, the localization of the Ca2+ pump in chick intestine, and the effect of dietary variables on the concentration of the pump, were studied. A monoclonal antibody produced against the human erythrocyte calcium pump was shown to cross-react with a chick intestinal Ca2+ pump epitope. The most intense staining of intestinal tissue, as determined immunohistochemically, occurred at the basolateral membrane of the duodenum, jejunum, ileum, and colon, with minor staining elsewhere. By the Western blotting procedure, vitamin D repletion of vitamin D-deficient chicks was shown to significantly increase the concentration of the Ca2+ pump epitope of duodenal, jejunal, and ileal mucosa by a factor of 2-3. Chicks were also fed diets deficient in calcium or phosphorus, a situation known to result in the stimulation of the synthesis of calbindin-D28k and an enhancement of the efficiency of Ca2+ absorption. Adaptation of the chicks to these deficient diets was verified by an increase in intestinal levels of calbindin-D28k, and is now shown to increase the Ca2+ pump epitope. From these immunological studies, it seems apparent that dietary variables that enhance intestinal Ca2+ absorption also increase the amount of the intestinal basolateral Ca2+ pump.

Original languageEnglish (US)
Pages (from-to)886-894
Number of pages9
JournalGastroenterology
Volume102
Issue number3
StatePublished - 1992

Fingerprint

Vitamin D Deficiency
Calbindin 1
Intestines
Minerals
Epitopes
Chickens
Cell Membrane
Calcium
Vitamin D
Staining and Labeling
Diet
Membranes
Enterocytes
Intestinal Absorption
Jejunum
Ileum
Duodenum
Phosphorus
Colon
Mucous Membrane

ASJC Scopus subject areas

  • Gastroenterology

Cite this

Wasserman, R. H., Smith, C. A., Brindak, M. E., Talamoni, N. D., Fullmer, C. S., Penniston, J. T., & Kumar, R. (1992). Vitamin D and mineral deficiencies increase the plasma membrane calcium pump of chicken intestine. Gastroenterology, 102(3), 886-894.

Vitamin D and mineral deficiencies increase the plasma membrane calcium pump of chicken intestine. / Wasserman, Robert H.; Smith, Christina A.; Brindak, Marie E.; Talamoni, Nori De; Fullmer, Curtis S.; Penniston, John T.; Kumar, Rajiv.

In: Gastroenterology, Vol. 102, No. 3, 1992, p. 886-894.

Research output: Contribution to journalArticle

Wasserman, RH, Smith, CA, Brindak, ME, Talamoni, ND, Fullmer, CS, Penniston, JT & Kumar, R 1992, 'Vitamin D and mineral deficiencies increase the plasma membrane calcium pump of chicken intestine', Gastroenterology, vol. 102, no. 3, pp. 886-894.
Wasserman RH, Smith CA, Brindak ME, Talamoni ND, Fullmer CS, Penniston JT et al. Vitamin D and mineral deficiencies increase the plasma membrane calcium pump of chicken intestine. Gastroenterology. 1992;102(3):886-894.
Wasserman, Robert H. ; Smith, Christina A. ; Brindak, Marie E. ; Talamoni, Nori De ; Fullmer, Curtis S. ; Penniston, John T. ; Kumar, Rajiv. / Vitamin D and mineral deficiencies increase the plasma membrane calcium pump of chicken intestine. In: Gastroenterology. 1992 ; Vol. 102, No. 3. pp. 886-894.
@article{256cade4e8a9429a93a80e64195b4dcb,
title = "Vitamin D and mineral deficiencies increase the plasma membrane calcium pump of chicken intestine",
abstract = "The basolateral membrane of the enterocyte was previously shown to contain an adenosine triphosphate-dependent calcium pump. Using immunological procedures, the localization of the Ca2+ pump in chick intestine, and the effect of dietary variables on the concentration of the pump, were studied. A monoclonal antibody produced against the human erythrocyte calcium pump was shown to cross-react with a chick intestinal Ca2+ pump epitope. The most intense staining of intestinal tissue, as determined immunohistochemically, occurred at the basolateral membrane of the duodenum, jejunum, ileum, and colon, with minor staining elsewhere. By the Western blotting procedure, vitamin D repletion of vitamin D-deficient chicks was shown to significantly increase the concentration of the Ca2+ pump epitope of duodenal, jejunal, and ileal mucosa by a factor of 2-3. Chicks were also fed diets deficient in calcium or phosphorus, a situation known to result in the stimulation of the synthesis of calbindin-D28k and an enhancement of the efficiency of Ca2+ absorption. Adaptation of the chicks to these deficient diets was verified by an increase in intestinal levels of calbindin-D28k, and is now shown to increase the Ca2+ pump epitope. From these immunological studies, it seems apparent that dietary variables that enhance intestinal Ca2+ absorption also increase the amount of the intestinal basolateral Ca2+ pump.",
author = "Wasserman, {Robert H.} and Smith, {Christina A.} and Brindak, {Marie E.} and Talamoni, {Nori De} and Fullmer, {Curtis S.} and Penniston, {John T.} and Rajiv Kumar",
year = "1992",
language = "English (US)",
volume = "102",
pages = "886--894",
journal = "Gastroenterology",
issn = "0016-5085",
publisher = "W.B. Saunders Ltd",
number = "3",

}

TY - JOUR

T1 - Vitamin D and mineral deficiencies increase the plasma membrane calcium pump of chicken intestine

AU - Wasserman, Robert H.

AU - Smith, Christina A.

AU - Brindak, Marie E.

AU - Talamoni, Nori De

AU - Fullmer, Curtis S.

AU - Penniston, John T.

AU - Kumar, Rajiv

PY - 1992

Y1 - 1992

N2 - The basolateral membrane of the enterocyte was previously shown to contain an adenosine triphosphate-dependent calcium pump. Using immunological procedures, the localization of the Ca2+ pump in chick intestine, and the effect of dietary variables on the concentration of the pump, were studied. A monoclonal antibody produced against the human erythrocyte calcium pump was shown to cross-react with a chick intestinal Ca2+ pump epitope. The most intense staining of intestinal tissue, as determined immunohistochemically, occurred at the basolateral membrane of the duodenum, jejunum, ileum, and colon, with minor staining elsewhere. By the Western blotting procedure, vitamin D repletion of vitamin D-deficient chicks was shown to significantly increase the concentration of the Ca2+ pump epitope of duodenal, jejunal, and ileal mucosa by a factor of 2-3. Chicks were also fed diets deficient in calcium or phosphorus, a situation known to result in the stimulation of the synthesis of calbindin-D28k and an enhancement of the efficiency of Ca2+ absorption. Adaptation of the chicks to these deficient diets was verified by an increase in intestinal levels of calbindin-D28k, and is now shown to increase the Ca2+ pump epitope. From these immunological studies, it seems apparent that dietary variables that enhance intestinal Ca2+ absorption also increase the amount of the intestinal basolateral Ca2+ pump.

AB - The basolateral membrane of the enterocyte was previously shown to contain an adenosine triphosphate-dependent calcium pump. Using immunological procedures, the localization of the Ca2+ pump in chick intestine, and the effect of dietary variables on the concentration of the pump, were studied. A monoclonal antibody produced against the human erythrocyte calcium pump was shown to cross-react with a chick intestinal Ca2+ pump epitope. The most intense staining of intestinal tissue, as determined immunohistochemically, occurred at the basolateral membrane of the duodenum, jejunum, ileum, and colon, with minor staining elsewhere. By the Western blotting procedure, vitamin D repletion of vitamin D-deficient chicks was shown to significantly increase the concentration of the Ca2+ pump epitope of duodenal, jejunal, and ileal mucosa by a factor of 2-3. Chicks were also fed diets deficient in calcium or phosphorus, a situation known to result in the stimulation of the synthesis of calbindin-D28k and an enhancement of the efficiency of Ca2+ absorption. Adaptation of the chicks to these deficient diets was verified by an increase in intestinal levels of calbindin-D28k, and is now shown to increase the Ca2+ pump epitope. From these immunological studies, it seems apparent that dietary variables that enhance intestinal Ca2+ absorption also increase the amount of the intestinal basolateral Ca2+ pump.

UR - http://www.scopus.com/inward/record.url?scp=0026500035&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026500035&partnerID=8YFLogxK

M3 - Article

C2 - 1311274

AN - SCOPUS:0026500035

VL - 102

SP - 886

EP - 894

JO - Gastroenterology

JF - Gastroenterology

SN - 0016-5085

IS - 3

ER -