Vitamin A concentrations in liver determined by isotope dilution assay with tetradeuterated vitamin A and by biopsy in generally healthy adult humans

H. C. Furr, O. Amedee-Manesme, A. J. Clifford, H. R. Bergen, A. D. Jones, D. P. Anderson, J. A. Olson

Research output: Contribution to journalArticle

110 Scopus citations

Abstract

The vitamin A status in 11 generally healthy surgical patients was estimated by measuring the dilution of a 45-mg oral dose of tetradeuterated retinyl acetate (99% pure). After purification of retinol by high-performance liquid chromatography, the ratio of 2H4-retinol:1H-retinol in plasma was measured by gas chromatography-mass spectrometry. On the basis of the observed ratios of [2H4]retinol:[1H]retinol over 19-47 d, the total body reserves and liver concentrations of vitamin A were calculated. Liver biopsy samples taken at surgery were directly analyzed for vitamin A. The correlation coefficient between calculated and measured liver vitamin A concentrations for 10 of the subjects was 0.88, and the Spearman's rank correlation coefficient was 0.95 (p < 0.002). Thus, total body reserves of vitamin A in humans can be estimated validly in the marginal and satisfactory ranges by a benign, relatively noninvasive procedure.

Original languageEnglish (US)
Pages (from-to)713-716
Number of pages4
JournalAmerican Journal of Clinical Nutrition
Volume49
Issue number4
DOIs
StatePublished - Jan 1 1989

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Nutrition and Dietetics

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