Visualization of three-dimensional nephron structure with microcomputed tomography

Michael D. Bentley, Steven M. Jorgensen, Lilach O Lerman, Erik L. Ritman, J. Carlos Romero

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

The three-dimensional architecture of nephrons in situ and their interrelationship with other nephrons are difficult to visualize by microscopic methods. The present study uses microcomputed X-ray tomography (micro-CT) to visualize intact nephrons in situ. Rat kidneys were perfusion-fixed with buffered formalin and their vasculature was subsequently perfused with radiopaque silicone. Cortical tissue was stained en bloc with osmium tetroxide, embedded in plastic, scanned, and reconstructed at voxel resolutions of 6, 2, and 1 μm. At 6 μm resolution, large blood vessels and glomeruli could be visualized but nephrons and their lumens were small and difficult to visualize. Optimal images were obtained using a synchrotron radiation source at 2 μm resolution where nephron components could be identified, correlated with histological sections, and traced. Proximal tubules had large diameters and opaque walls, whereas distal tubules, connecting tubules, and collecting ducts had smaller diameters and less opaque walls. Blood vessels could be distinguished from nephrons by the luminal presence of radiopaque silicone. Proximal tubules were three times longer than distal tubules. Proximal and distal tubules were tightly coiled in the outer cortex but were loosely coiled in the middle and inner cortex. The connecting tubules had the narrowest diameters of the tubules and converged to form arcades that paralleled the radial vessels as they extended to the outer cortex. These results illustrate a potential use of micro-CT to obtain three-dimensional information about nephron architecture and nephron interrelationships, which could be useful in evaluating experimental tubular hypertrophy, atrophy, and necrosis.

Original languageEnglish (US)
Pages (from-to)277-283
Number of pages7
JournalAnatomical Record
Volume290
Issue number3
DOIs
StatePublished - Mar 2007

Fingerprint

micro-computed tomography
X-Ray Microtomography
nephrons
Nephrons
tomography
visualization
vessel
blood
osmium
cortex
proximal tubules
silicone
Silicones
plastic
blood vessels
Blood Vessels
X-radiation
Osmium Tetroxide
Synchrotrons
atrophy

Keywords

  • Imaging
  • Kidney
  • Osmium
  • Renal tubules
  • Tomography

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Biotechnology
  • Anatomy
  • Histology

Cite this

Visualization of three-dimensional nephron structure with microcomputed tomography. / Bentley, Michael D.; Jorgensen, Steven M.; Lerman, Lilach O; Ritman, Erik L.; Romero, J. Carlos.

In: Anatomical Record, Vol. 290, No. 3, 03.2007, p. 277-283.

Research output: Contribution to journalArticle

Bentley, Michael D. ; Jorgensen, Steven M. ; Lerman, Lilach O ; Ritman, Erik L. ; Romero, J. Carlos. / Visualization of three-dimensional nephron structure with microcomputed tomography. In: Anatomical Record. 2007 ; Vol. 290, No. 3. pp. 277-283.
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