TY - JOUR
T1 - Visualization of Oryza eichingeri chromosomes in intergenomic hybrid plants from O. sativa x O. eichingeri via fluorescent in situ hybridization
AU - Yan, H.
AU - Min, S.
AU - Zhu, L.
PY - 1999/1/1
Y1 - 1999/1/1
N2 - Digoxigenin-labeled total genomic DNA from Oryza eichingeri was hybridized in situ to somatic chromosome preparations of F1, F2, backcross progenies, and a pollen-derived tetraploid plant (E24) from O. sativa (2n = 24, genome AA) x O. eichingeri (2n = 24, genome CC), which allowed a definitive discrimination between A- and C-genome chromosomes. Twelve chromosomes in F1, F2, BC1, and twenty-four chromosomes in plant E24 were clearly revealed to be of O. eichingeri origin, thus confirming that both BC1 and F2 were allotriploids (2n = 36, AAC) while plant E24 was an amphiploid (2n = 48, AACC). In addition, the presence of O. eichingeri chromosomes in four alien addition lines was characterized. The results suggest that FISH/GISH may be a powerful tool for monitoring the O. sativa-alien chromosome in the progenies of rice interspecific hybridization.
AB - Digoxigenin-labeled total genomic DNA from Oryza eichingeri was hybridized in situ to somatic chromosome preparations of F1, F2, backcross progenies, and a pollen-derived tetraploid plant (E24) from O. sativa (2n = 24, genome AA) x O. eichingeri (2n = 24, genome CC), which allowed a definitive discrimination between A- and C-genome chromosomes. Twelve chromosomes in F1, F2, BC1, and twenty-four chromosomes in plant E24 were clearly revealed to be of O. eichingeri origin, thus confirming that both BC1 and F2 were allotriploids (2n = 36, AAC) while plant E24 was an amphiploid (2n = 48, AACC). In addition, the presence of O. eichingeri chromosomes in four alien addition lines was characterized. The results suggest that FISH/GISH may be a powerful tool for monitoring the O. sativa-alien chromosome in the progenies of rice interspecific hybridization.
KW - Alien addition lines
KW - Amphiploid
KW - Fluorescent in situ hybridization
KW - Oryza eichingeri
KW - Oryza sativa
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U2 - 10.1139/g98-096
DO - 10.1139/g98-096
M3 - Article
AN - SCOPUS:0032955691
VL - 42
SP - 48
EP - 51
JO - Genome / National Research Council Canada = Genome / Conseil national de recherches Canada
JF - Genome / National Research Council Canada = Genome / Conseil national de recherches Canada
SN - 0831-2796
IS - 1
ER -