Abstract
Digoxigenin-labeled total genomic DNA from Oryza eichingeri was hybridized in situ to somatic chromosome preparations of F1, F2, backcross progenies, and a pollen-derived tetraploid plant (E24) from O. sativa (2n = 24, genome AA) x O. eichingeri (2n = 24, genome CC), which allowed a definitive discrimination between A- and C-genome chromosomes. Twelve chromosomes in F1, F2, BC1, and twenty-four chromosomes in plant E24 were clearly revealed to be of O. eichingeri origin, thus confirming that both BC1 and F2 were allotriploids (2n = 36, AAC) while plant E24 was an amphiploid (2n = 48, AACC). In addition, the presence of O. eichingeri chromosomes in four alien addition lines was characterized. The results suggest that FISH/GISH may be a powerful tool for monitoring the O. sativa-alien chromosome in the progenies of rice interspecific hybridization.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 48-51 |
| Number of pages | 4 |
| Journal | Genome |
| Volume | 42 |
| Issue number | 1 |
| State | Published - 1999 |
| Externally published | Yes |
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Keywords
- Alien addition lines
- Amphiploid
- Fluorescent in situ hybridization
- Oryza eichingeri
- Oryza sativa
ASJC Scopus subject areas
- Agricultural and Biological Sciences(all)
- Biotechnology
- Genetics
- Genetics(clinical)