Visualization of Oryza eichingeri chromosomes in intergenomic hybrid plants from O. sativa x O. eichingeri via fluorescent in situ hybridization

H. Yan; S. Min; L. Zhu

doi:10.1139/g98-096

Visualization of Oryza eichingeri chromosomes in intergenomic hybrid plants from O. sativa x O. eichingeri via fluorescent in situ hybridization

H. Yan, S. Min, L. Zhu

Research output: Contribution to journal › Article › peer-review

16 Scopus citations

Abstract

Digoxigenin-labeled total genomic DNA from Oryza eichingeri was hybridized in situ to somatic chromosome preparations of F₁, F₂, backcross progenies, and a pollen-derived tetraploid plant (E₂₄) from O. sativa (2n = 24, genome AA) x O. eichingeri (2n = 24, genome CC), which allowed a definitive discrimination between A- and C-genome chromosomes. Twelve chromosomes in F₁, F₂, BC₁, and twenty-four chromosomes in plant E₂₄ were clearly revealed to be of O. eichingeri origin, thus confirming that both BC₁ and F₂ were allotriploids (2n = 36, AAC) while plant E₂₄ was an amphiploid (2n = 48, AACC). In addition, the presence of O. eichingeri chromosomes in four alien addition lines was characterized. The results suggest that FISH/GISH may be a powerful tool for monitoring the O. sativa-alien chromosome in the progenies of rice interspecific hybridization.

Original language	English (US)
Pages (from-to)	48-51
Number of pages	4
Journal	Genome
Volume	42
Issue number	1
DOIs	https://doi.org/10.1139/g98-096
State	Published - 1999

Keywords

Alien addition lines
Amphiploid
Fluorescent in situ hybridization
Oryza eichingeri
Oryza sativa

ASJC Scopus subject areas

Biotechnology
Molecular Biology
Genetics

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title = "Visualization of Oryza eichingeri chromosomes in intergenomic hybrid plants from O. sativa x O. eichingeri via fluorescent in situ hybridization",

abstract = "Digoxigenin-labeled total genomic DNA from Oryza eichingeri was hybridized in situ to somatic chromosome preparations of F1, F2, backcross progenies, and a pollen-derived tetraploid plant (E24) from O. sativa (2n = 24, genome AA) x O. eichingeri (2n = 24, genome CC), which allowed a definitive discrimination between A- and C-genome chromosomes. Twelve chromosomes in F1, F2, BC1, and twenty-four chromosomes in plant E24 were clearly revealed to be of O. eichingeri origin, thus confirming that both BC1 and F2 were allotriploids (2n = 36, AAC) while plant E24 was an amphiploid (2n = 48, AACC). In addition, the presence of O. eichingeri chromosomes in four alien addition lines was characterized. The results suggest that FISH/GISH may be a powerful tool for monitoring the O. sativa-alien chromosome in the progenies of rice interspecific hybridization.",

keywords = "Alien addition lines, Amphiploid, Fluorescent in situ hybridization, Oryza eichingeri, Oryza sativa",

author = "H. Yan and S. Min and L. Zhu",

year = "1999",

doi = "10.1139/g98-096",

language = "English (US)",

volume = "42",

pages = "48--51",

journal = "Genome",

issn = "0831-2796",

publisher = "National Research Council of Canada",

number = "1",

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T1 - Visualization of Oryza eichingeri chromosomes in intergenomic hybrid plants from O. sativa x O. eichingeri via fluorescent in situ hybridization

AU - Yan, H.

AU - Min, S.

AU - Zhu, L.

PY - 1999

Y1 - 1999

N2 - Digoxigenin-labeled total genomic DNA from Oryza eichingeri was hybridized in situ to somatic chromosome preparations of F1, F2, backcross progenies, and a pollen-derived tetraploid plant (E24) from O. sativa (2n = 24, genome AA) x O. eichingeri (2n = 24, genome CC), which allowed a definitive discrimination between A- and C-genome chromosomes. Twelve chromosomes in F1, F2, BC1, and twenty-four chromosomes in plant E24 were clearly revealed to be of O. eichingeri origin, thus confirming that both BC1 and F2 were allotriploids (2n = 36, AAC) while plant E24 was an amphiploid (2n = 48, AACC). In addition, the presence of O. eichingeri chromosomes in four alien addition lines was characterized. The results suggest that FISH/GISH may be a powerful tool for monitoring the O. sativa-alien chromosome in the progenies of rice interspecific hybridization.

AB - Digoxigenin-labeled total genomic DNA from Oryza eichingeri was hybridized in situ to somatic chromosome preparations of F1, F2, backcross progenies, and a pollen-derived tetraploid plant (E24) from O. sativa (2n = 24, genome AA) x O. eichingeri (2n = 24, genome CC), which allowed a definitive discrimination between A- and C-genome chromosomes. Twelve chromosomes in F1, F2, BC1, and twenty-four chromosomes in plant E24 were clearly revealed to be of O. eichingeri origin, thus confirming that both BC1 and F2 were allotriploids (2n = 36, AAC) while plant E24 was an amphiploid (2n = 48, AACC). In addition, the presence of O. eichingeri chromosomes in four alien addition lines was characterized. The results suggest that FISH/GISH may be a powerful tool for monitoring the O. sativa-alien chromosome in the progenies of rice interspecific hybridization.

KW - Alien addition lines

KW - Amphiploid

KW - Fluorescent in situ hybridization

KW - Oryza eichingeri

KW - Oryza sativa

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Visualization of Oryza eichingeri chromosomes in intergenomic hybrid plants from O. sativa x O. eichingeri via fluorescent in situ hybridization

Abstract

Keywords

ASJC Scopus subject areas

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