Viral neuraminidase treatment of dendritic cells enhances antigen-specific CD8+ T cell proliferation, but does not account for the CD4+ T cell independence of the CD8+ T cell response during influenza virus infection

Sang Kon Oh, Gabrielle T. Belz, Maryna C. Eichelberger

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

In vitro studies demonstrate that the increased alloreactive T cell response to dendritic cells (DC) that are treated with either live or inactivated influenza virus A/PR/8/34 is due to viral neuraminidase (NA) activity. Since virus-specific cytotoxic T lymphocytes (CTL) play an important role in immunity to heterologous influenza strains, we compared the activation of CD8+ T cells by untreated and NA-treated DC. Increased CTL activity was induced by NA-treated DC both in vitro and in vivo. Since the generation of CTL in response to influenza virus infection does not require prior "activation" of DC by CD4+ T cells (as is the case for many antigens), we asked whether NA activity contributed to this unconditional CD8+ T cell response. This was not the case. Future studies will determine the factors that are responsible for the CD4+ T-cell-independent influenza virus-specific CTL response.

Original languageEnglish (US)
Pages (from-to)403-411
Number of pages9
JournalVirology
Volume286
Issue number2
DOIs
StatePublished - Aug 1 2001

Keywords

  • CD8 T cells
  • Cytotoxic T lymphocytes
  • Dendritic cells
  • ICAM-1
  • Influenza virus
  • Neuraminidase
  • Sialic acid
  • Vaccines

ASJC Scopus subject areas

  • Virology

Fingerprint Dive into the research topics of 'Viral neuraminidase treatment of dendritic cells enhances antigen-specific CD8<sup>+</sup> T cell proliferation, but does not account for the CD4<sup>+</sup> T cell independence of the CD8<sup>+</sup> T cell response during influenza virus infection'. Together they form a unique fingerprint.

  • Cite this