Viral neuraminidase treatment of dendritic cells enhances antigen-specific CD8+ T cell proliferation, but does not account for the CD4+ T cell independence of the CD8+ T cell response during influenza virus infection

SangKon Oh, Gabrielle T. Belz, Maryna C. Eichelberger

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

In vitro studies demonstrate that the increased alloreactive T cell response to dendritic cells (DC) that are treated with either live or inactivated influenza virus A/PR/8/34 is due to viral neuraminidase (NA) activity. Since virus-specific cytotoxic T lymphocytes (CTL) play an important role in immunity to heterologous influenza strains, we compared the activation of CD8+ T cells by untreated and NA-treated DC. Increased CTL activity was induced by NA-treated DC both in vitro and in vivo. Since the generation of CTL in response to influenza virus infection does not require prior "activation" of DC by CD4+ T cells (as is the case for many antigens), we asked whether NA activity contributed to this unconditional CD8+ T cell response. This was not the case. Future studies will determine the factors that are responsible for the CD4+ T-cell-independent influenza virus-specific CTL response.

Original languageEnglish (US)
Pages (from-to)403-411
Number of pages9
JournalVirology
Volume286
Issue number2
DOIs
StatePublished - Aug 1 2001
Externally publishedYes

Fingerprint

CD8 Antigens
Neuraminidase
Virus Diseases
Orthomyxoviridae
Dendritic Cells
Cytotoxic T-Lymphocytes
Cell Proliferation
T-Lymphocytes
Heterologous Immunity
Influenza A virus
Human Influenza
Viruses
Antigens

Keywords

  • CD8 T cells
  • Cytotoxic T lymphocytes
  • Dendritic cells
  • ICAM-1
  • Influenza virus
  • Neuraminidase
  • Sialic acid
  • Vaccines

ASJC Scopus subject areas

  • Virology

Cite this

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abstract = "In vitro studies demonstrate that the increased alloreactive T cell response to dendritic cells (DC) that are treated with either live or inactivated influenza virus A/PR/8/34 is due to viral neuraminidase (NA) activity. Since virus-specific cytotoxic T lymphocytes (CTL) play an important role in immunity to heterologous influenza strains, we compared the activation of CD8+ T cells by untreated and NA-treated DC. Increased CTL activity was induced by NA-treated DC both in vitro and in vivo. Since the generation of CTL in response to influenza virus infection does not require prior {"}activation{"} of DC by CD4+ T cells (as is the case for many antigens), we asked whether NA activity contributed to this unconditional CD8+ T cell response. This was not the case. Future studies will determine the factors that are responsible for the CD4+ T-cell-independent influenza virus-specific CTL response.",
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AU - Oh, SangKon

AU - Belz, Gabrielle T.

AU - Eichelberger, Maryna C.

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N2 - In vitro studies demonstrate that the increased alloreactive T cell response to dendritic cells (DC) that are treated with either live or inactivated influenza virus A/PR/8/34 is due to viral neuraminidase (NA) activity. Since virus-specific cytotoxic T lymphocytes (CTL) play an important role in immunity to heterologous influenza strains, we compared the activation of CD8+ T cells by untreated and NA-treated DC. Increased CTL activity was induced by NA-treated DC both in vitro and in vivo. Since the generation of CTL in response to influenza virus infection does not require prior "activation" of DC by CD4+ T cells (as is the case for many antigens), we asked whether NA activity contributed to this unconditional CD8+ T cell response. This was not the case. Future studies will determine the factors that are responsible for the CD4+ T-cell-independent influenza virus-specific CTL response.

AB - In vitro studies demonstrate that the increased alloreactive T cell response to dendritic cells (DC) that are treated with either live or inactivated influenza virus A/PR/8/34 is due to viral neuraminidase (NA) activity. Since virus-specific cytotoxic T lymphocytes (CTL) play an important role in immunity to heterologous influenza strains, we compared the activation of CD8+ T cells by untreated and NA-treated DC. Increased CTL activity was induced by NA-treated DC both in vitro and in vivo. Since the generation of CTL in response to influenza virus infection does not require prior "activation" of DC by CD4+ T cells (as is the case for many antigens), we asked whether NA activity contributed to this unconditional CD8+ T cell response. This was not the case. Future studies will determine the factors that are responsible for the CD4+ T-cell-independent influenza virus-specific CTL response.

KW - CD8 T cells

KW - Cytotoxic T lymphocytes

KW - Dendritic cells

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KW - Influenza virus

KW - Neuraminidase

KW - Sialic acid

KW - Vaccines

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