Viral attachment induces rapid recruitment of an innate immune sensor (TRIM5α) to the plasma membrane

Seiga Ohmine, Raman Deep Singh, David L. Marks, Melissa A. Meyer, Richard E. Pagano, Yasuhiro H Ikeda

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

TRIM5α (tripartite motif 5α) acts as a pattern recognition receptor specific for the retrovirus capsid lattice and blocks infection by HIV-1 immediately after entry. However, the precise mechanisms underlying this rapid recognition of viral components remain elusive. Here, we analyzed the influence of viral exposure on TRIM5α. Total internal reflection fluorescence microscopy and lipid flotation assays revealed rapid recruitment of a TRIM5α subpopulation to the plasma membrane (PM) upon exposure to vesicular stomatitis virus-G-pseudotyped HIV-1 viral-like particles (VLPs), but not to envelope (Env)-less HIV-1 VLPs. TRIM5α signals were frequently colocalized with those of HIV-1 capsid at the PM. Exposure to HIV-1 Env-pseudotyped HIV-1 vectors also triggered translocation of endogenous TRIM5α to lipid microdomains within human T cells. Similarly, clustering of lipid microdomains by a glycosphingolipid stereoisomer resulted in rapid TRIM5α recruitment to the PM. Of note, recruitment of endogenous rhesus TRIM5α to the PM prior to HIV-1 infection significantly increased the potency of viral restriction. Our data therefore suggest the importance of TRIM5α recruitment to the PM for TRIM5α-mediated innate immune sensing and restriction of retroviral infection.

Original languageEnglish (US)
Pages (from-to)414-424
Number of pages11
JournalJournal of Innate Immunity
Volume5
Issue number4
DOIs
StatePublished - Jun 2013

Fingerprint

HIV-1
Cell Membrane
Capsid
Lipids
Virion
Pattern Recognition Receptors
Viral Structures
Glycosphingolipids
Vesicular Stomatitis
Stereoisomerism
Retroviridae
Infection
Fluorescence Microscopy
HIV Infections
Cluster Analysis
Viruses
T-Lymphocytes

Keywords

  • HIV
  • Innate immunity
  • Membrane rafts
  • Plasma membrane
  • Restriction factor
  • TRIM5α

ASJC Scopus subject areas

  • Immunology and Allergy

Cite this

Ohmine, S., Singh, R. D., Marks, D. L., Meyer, M. A., Pagano, R. E., & Ikeda, Y. H. (2013). Viral attachment induces rapid recruitment of an innate immune sensor (TRIM5α) to the plasma membrane. Journal of Innate Immunity, 5(4), 414-424. https://doi.org/10.1159/000346963

Viral attachment induces rapid recruitment of an innate immune sensor (TRIM5α) to the plasma membrane. / Ohmine, Seiga; Singh, Raman Deep; Marks, David L.; Meyer, Melissa A.; Pagano, Richard E.; Ikeda, Yasuhiro H.

In: Journal of Innate Immunity, Vol. 5, No. 4, 06.2013, p. 414-424.

Research output: Contribution to journalArticle

Ohmine, Seiga ; Singh, Raman Deep ; Marks, David L. ; Meyer, Melissa A. ; Pagano, Richard E. ; Ikeda, Yasuhiro H. / Viral attachment induces rapid recruitment of an innate immune sensor (TRIM5α) to the plasma membrane. In: Journal of Innate Immunity. 2013 ; Vol. 5, No. 4. pp. 414-424.
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