TY - JOUR
T1 - Very-low-density lipoprotein subfraction composition and metabolism by adipose tissue
AU - Fisher, Rachel M.
AU - Miles, John M.
AU - Kottke, Bruce A.
AU - Frayn, Keith N.
AU - Coppack, Simon W.
N1 - Funding Information:
From the Endocrine and Atherosclerosis Research Units, Mayo Clinic, Rochester, MN; Oxford Lipid Metabolism Group, Radcliffe Infirmary, Oxford; and Department of Medicine, University College London Medical School, Rayne Institute, London, UK. Submitted September 2, \]995; accepted December 22, 1996. Supported in part by grants from the US Public Health Service (DK38092 and RR00585) and a British Heart Foundation Research Studentship (R.M.E ). Address reprint requests to Rachel M. Fisher, D Phil, Division of Cardiovascular Genetics, Department of Medicine, University College London Medical School, Rayne Institute, London, WC1E 6JJ UK. Copyright © 1997 by W.B. Saunders Company 0026-0495/97/4606-0002503.00/0
Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.
PY - 1997
Y1 - 1997
N2 - Lipoprotein lipase (LPL) plays a pivotal role in very-low-density lipoprotein (VLDL) metabolism. Within the circulation, the VLDL population is heterogeneous with respect to both size and composition. Several studies have investigated the action of LPL in vitro on different VLDL subfractions, but little is known of the action of LPL in vivo. To investigate this, arterial end adipose tissue venous plasma samples were obtained from 16 normal male healthy volunteers (aged 24.4 ± 1.8 years; body mass index, 23.5 ± 0.7 kg · m-2) following an overnight fast. VLDL subfractions were isolated (VLDL1 of S(f) 60 to 400 and VLDL2 of S(f) 20 to 60) end characterized in terms of triacylglycerol (TAG) and apolipoprotein (apo) B, E, CI, CII, and CIII content. The apolipoprotein content of VLDL1 differed from that of VLDL2: the VLDL2 fraction contained significantly more apo B (0.018 ± 0.004 v 0.011 ± 0.003 μmol · L-1, P = .001) but the ratios of TAG:apo B and apo CI:B, CII:B, and CIII:B were significantly higher in VLDL1 (48,200 ± 7,960 v 13,860 ± 2,420, 22.7 ± 5.5 v 12.5 ± 2.2, 45.0 ± 6.3 v 14.9 ± 2.0, and 0.434 ± 0.077 v 0.357 ± 0.054, respectively, molar ratios, all P < .05). The venous blood draining an adipose tissue depot contained less VLDL1-TAG than arterial blood (328 ± 68 v 381 ± 83 μmol · L-1, respectively, PC .01), whereas VLDL2-TAG exhibited an opposite tendency (199 ± 46 v 172 ± 31 μmol · L-1, NS). Concentrations of VLDL1-apo B, -apo CII, and -apo CIII were significantly less in adipose tissue venous blood compared with arterial blood (0.011 ± 0.004 v 0.013 ± 0.004, 0.38 ± 0.08 v 0.43 ± 0.10, and 1.33 ± 0.35 v 1.58 ± 0.38 μmol · L-1, respectively, all P <: .05). These studies demonstrated novel differences in VLDL1 and VLDL2 in terms of composition and metabolism by human adipose tissue LPL in vivo.
AB - Lipoprotein lipase (LPL) plays a pivotal role in very-low-density lipoprotein (VLDL) metabolism. Within the circulation, the VLDL population is heterogeneous with respect to both size and composition. Several studies have investigated the action of LPL in vitro on different VLDL subfractions, but little is known of the action of LPL in vivo. To investigate this, arterial end adipose tissue venous plasma samples were obtained from 16 normal male healthy volunteers (aged 24.4 ± 1.8 years; body mass index, 23.5 ± 0.7 kg · m-2) following an overnight fast. VLDL subfractions were isolated (VLDL1 of S(f) 60 to 400 and VLDL2 of S(f) 20 to 60) end characterized in terms of triacylglycerol (TAG) and apolipoprotein (apo) B, E, CI, CII, and CIII content. The apolipoprotein content of VLDL1 differed from that of VLDL2: the VLDL2 fraction contained significantly more apo B (0.018 ± 0.004 v 0.011 ± 0.003 μmol · L-1, P = .001) but the ratios of TAG:apo B and apo CI:B, CII:B, and CIII:B were significantly higher in VLDL1 (48,200 ± 7,960 v 13,860 ± 2,420, 22.7 ± 5.5 v 12.5 ± 2.2, 45.0 ± 6.3 v 14.9 ± 2.0, and 0.434 ± 0.077 v 0.357 ± 0.054, respectively, molar ratios, all P < .05). The venous blood draining an adipose tissue depot contained less VLDL1-TAG than arterial blood (328 ± 68 v 381 ± 83 μmol · L-1, respectively, PC .01), whereas VLDL2-TAG exhibited an opposite tendency (199 ± 46 v 172 ± 31 μmol · L-1, NS). Concentrations of VLDL1-apo B, -apo CII, and -apo CIII were significantly less in adipose tissue venous blood compared with arterial blood (0.011 ± 0.004 v 0.013 ± 0.004, 0.38 ± 0.08 v 0.43 ± 0.10, and 1.33 ± 0.35 v 1.58 ± 0.38 μmol · L-1, respectively, all P <: .05). These studies demonstrated novel differences in VLDL1 and VLDL2 in terms of composition and metabolism by human adipose tissue LPL in vivo.
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U2 - 10.1016/S0026-0495(97)90001-9
DO - 10.1016/S0026-0495(97)90001-9
M3 - Article
C2 - 9186293
AN - SCOPUS:0030919969
SN - 0026-0495
VL - 46
SP - 605
EP - 610
JO - Metabolism: Clinical and Experimental
JF - Metabolism: Clinical and Experimental
IS - 6
ER -