Very-low-density lipoprotein subfraction composition and metabolism by adipose tissue

Rachel M. Fisher, John M. Miles, Bruce A. Kottke, Keith N. Frayn, Simon W. Coppack

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Lipoprotein lipase (LPL) plays a pivotal role in very-low-density lipoprotein (VLDL) metabolism. Within the circulation, the VLDL population is heterogeneous with respect to both size and composition. Several studies have investigated the action of LPL in vitro on different VLDL subfractions, but little is known of the action of LPL in vivo. To investigate this, arterial end adipose tissue venous plasma samples were obtained from 16 normal male healthy volunteers (aged 24.4 ± 1.8 years; body mass index, 23.5 ± 0.7 kg · m-2) following an overnight fast. VLDL subfractions were isolated (VLDL1 of S(f) 60 to 400 and VLDL2 of S(f) 20 to 60) end characterized in terms of triacylglycerol (TAG) and apolipoprotein (apo) B, E, CI, CII, and CIII content. The apolipoprotein content of VLDL1 differed from that of VLDL2: the VLDL2 fraction contained significantly more apo B (0.018 ± 0.004 v 0.011 ± 0.003 μmol · L-1, P = .001) but the ratios of TAG:apo B and apo CI:B, CII:B, and CIII:B were significantly higher in VLDL1 (48,200 ± 7,960 v 13,860 ± 2,420, 22.7 ± 5.5 v 12.5 ± 2.2, 45.0 ± 6.3 v 14.9 ± 2.0, and 0.434 ± 0.077 v 0.357 ± 0.054, respectively, molar ratios, all P < .05). The venous blood draining an adipose tissue depot contained less VLDL1-TAG than arterial blood (328 ± 68 v 381 ± 83 μmol · L-1, respectively, PC .01), whereas VLDL2-TAG exhibited an opposite tendency (199 ± 46 v 172 ± 31 μmol · L-1, NS). Concentrations of VLDL1-apo B, -apo CII, and -apo CIII were significantly less in adipose tissue venous blood compared with arterial blood (0.011 ± 0.004 v 0.013 ± 0.004, 0.38 ± 0.08 v 0.43 ± 0.10, and 1.33 ± 0.35 v 1.58 ± 0.38 μmol · L-1, respectively, all P <: .05). These studies demonstrated novel differences in VLDL1 and VLDL2 in terms of composition and metabolism by human adipose tissue LPL in vivo.

Original languageEnglish (US)
Pages (from-to)605-610
Number of pages6
JournalMetabolism: Clinical and Experimental
Volume46
Issue number6
DOIs
StatePublished - 1997
Externally publishedYes

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VLDL Lipoproteins
Apolipoproteins B
Lipoprotein Lipase
Adipose Tissue
Triglycerides
Apolipoprotein C-II
Apolipoprotein C-III
Apolipoproteins
Apolipoproteins E
Healthy Volunteers
Body Mass Index
Population

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Very-low-density lipoprotein subfraction composition and metabolism by adipose tissue. / Fisher, Rachel M.; Miles, John M.; Kottke, Bruce A.; Frayn, Keith N.; Coppack, Simon W.

In: Metabolism: Clinical and Experimental, Vol. 46, No. 6, 1997, p. 605-610.

Research output: Contribution to journalArticle

Fisher, Rachel M. ; Miles, John M. ; Kottke, Bruce A. ; Frayn, Keith N. ; Coppack, Simon W. / Very-low-density lipoprotein subfraction composition and metabolism by adipose tissue. In: Metabolism: Clinical and Experimental. 1997 ; Vol. 46, No. 6. pp. 605-610.
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AU - Coppack, Simon W.

PY - 1997

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