Vertebrate development requires ARVCF and p120 catenins and their interplay with RhoA and Rac

Xiang Fang, Hong Ji, Si Wan Kim, Jae Il Park, Travis G. Vaught, Panos Z. Anastasiadis, Malgorzata Ciesiolka, Pierre D. McCrea

Research output: Contribution to journalArticle

76 Scopus citations

Abstract

Using an animal model system and depletion-rescue strategies, we have addressed the requirement and functions of armadillo repeat gene deleted in velo-cardio-facial syndrome (ARVCF) and p120 catenins in early vertebrate embryogenesis. We find that xARVCF and Xp120 are essential to development given that depletion of either results in disrupted gastrulation and axial elongation, which are specific phenotypes based on self-rescue analysis and further criteria. Exogenous xARVCF or Xp120 cross-rescued depletion of the other, and each depletion was additionally rescued with (carefully titrated) dominant-negative RhoA or dominant-active Rac. Although xARVCF or Xp120 depletion did not appear to reduce the adhesive function of C-cadherin in standard cell reaggregation and additional assays, C-cadherin levels were somewhat reduced after xARVCF or Xp120 depletion, and rescue analysis using partial or full-length C-cadherin constructs suggested contributory effects on altered adhesion and signaling functions. This work indicates the required functions of both p120 and ARVCF in vertebrate embryogenesis and their shared functional interplay with RhoA, Rac, and cadherin in a developmental context.

Original languageEnglish (US)
Pages (from-to)87-98
Number of pages12
JournalJournal of Cell Biology
Volume165
Issue number1
DOIs
StatePublished - Apr 12 2004

Keywords

  • Actin cytoskeleton
  • Cell adhesion
  • Cell motility
  • GTPase
  • Morphogenesis

ASJC Scopus subject areas

  • Cell Biology

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    Fang, X., Ji, H., Kim, S. W., Park, J. I., Vaught, T. G., Anastasiadis, P. Z., Ciesiolka, M., & McCrea, P. D. (2004). Vertebrate development requires ARVCF and p120 catenins and their interplay with RhoA and Rac. Journal of Cell Biology, 165(1), 87-98. https://doi.org/10.1083/jcb.200307109