TY - JOUR
T1 - Ventricular cardiotrophin-1 activation precedes BNP in experimental heart failure
AU - Jougasaki, Michihisa
AU - Leskinen, Hanna
AU - Larsen, Amy M.
AU - Luchner, Andreas
AU - Cataliotti, Alessandro
AU - Tachibana, Issei
AU - Burnett, John C.
N1 - Funding Information:
We acknowledge the technical assistance of Gail J. Harty, Denise M. Heublein and Sharon M. Sandberg. This work was supported by grants from Japan Cardiovascular Research Foundation, the National Institute of Health (HL 36634), the Miami Heart Research Institute and Mayo Foundation.
PY - 2003/6/1
Y1 - 2003/6/1
N2 - Both cardiotrophin-1 (CT-1) and B-type or brain natriuretic peptide (BNP) are activated by cardiomyocyte stretch, and gene expression of CT-1 and BNP are augmented in the heart in experimental and human congestive heart failure (CHF). The goal of this study was to define cardiac gene expression of CT-1 and BNP by Northern blot analysis in normal (n=5), early left ventricular dysfunction (ELVD, n=5) and overt CHF dogs (n=5), in which ventricular function is progressively decreased. CT-1 mRNA was detected in both atria and ventricles in normal dogs. Ventricular CT-1 mRNA production increased in ELVD, and it further increased in overt CHF. Ventricular BNP mRNA remained below or at the limit of detection in normal and ELVD models, and it markedly increased in overt CHF. This study reports differential regulation of gene expression of CT-1 and BNP in the heart during the progression of CHF, and demonstrates that ventricular CT-1 gene activation precedes ventricular BNP gene activation.
AB - Both cardiotrophin-1 (CT-1) and B-type or brain natriuretic peptide (BNP) are activated by cardiomyocyte stretch, and gene expression of CT-1 and BNP are augmented in the heart in experimental and human congestive heart failure (CHF). The goal of this study was to define cardiac gene expression of CT-1 and BNP by Northern blot analysis in normal (n=5), early left ventricular dysfunction (ELVD, n=5) and overt CHF dogs (n=5), in which ventricular function is progressively decreased. CT-1 mRNA was detected in both atria and ventricles in normal dogs. Ventricular CT-1 mRNA production increased in ELVD, and it further increased in overt CHF. Ventricular BNP mRNA remained below or at the limit of detection in normal and ELVD models, and it markedly increased in overt CHF. This study reports differential regulation of gene expression of CT-1 and BNP in the heart during the progression of CHF, and demonstrates that ventricular CT-1 gene activation precedes ventricular BNP gene activation.
KW - BNP
KW - Cardiotrophin-1
KW - Congestive heart failure
KW - Cytokines
KW - Northern blot analysis
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U2 - 10.1016/S0196-9781(03)00163-3
DO - 10.1016/S0196-9781(03)00163-3
M3 - Article
C2 - 12948841
AN - SCOPUS:0042430588
SN - 0196-9781
VL - 24
SP - 889
EP - 892
JO - Peptides
JF - Peptides
IS - 6
ER -