Ventricular cardiotrophin-1 activation precedes BNP in experimental heart failure

Michihisa Jougasaki, Hanna Leskinen, Amy M. Larsen, Andreas Luchner, Alessandro Cataliotti, Issei Tachibana, John C Jr. Burnett

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Both cardiotrophin-1 (CT-1) and B-type or brain natriuretic peptide (BNP) are activated by cardiomyocyte stretch, and gene expression of CT-1 and BNP are augmented in the heart in experimental and human congestive heart failure (CHF). The goal of this study was to define cardiac gene expression of CT-1 and BNP by Northern blot analysis in normal (n=5), early left ventricular dysfunction (ELVD, n=5) and overt CHF dogs (n=5), in which ventricular function is progressively decreased. CT-1 mRNA was detected in both atria and ventricles in normal dogs. Ventricular CT-1 mRNA production increased in ELVD, and it further increased in overt CHF. Ventricular BNP mRNA remained below or at the limit of detection in normal and ELVD models, and it markedly increased in overt CHF. This study reports differential regulation of gene expression of CT-1 and BNP in the heart during the progression of CHF, and demonstrates that ventricular CT-1 gene activation precedes ventricular BNP gene activation.

Original languageEnglish (US)
Pages (from-to)889-892
Number of pages4
JournalPeptides
Volume24
Issue number6
DOIs
StatePublished - Jun 1 2003

Fingerprint

Brain Natriuretic Peptide
Heart Failure
Chemical activation
Gene expression
Messenger RNA
Transcriptional Activation
Genes
Dogs
Gene Expression
Ventricular Function
Gene Expression Regulation
Left Ventricular Dysfunction
cardiotrophin 1
Cardiac Myocytes
Northern Blotting
Limit of Detection

Keywords

  • BNP
  • Cardiotrophin-1
  • Congestive heart failure
  • Cytokines
  • Northern blot analysis

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology
  • Physiology
  • Cellular and Molecular Neuroscience

Cite this

Jougasaki, M., Leskinen, H., Larsen, A. M., Luchner, A., Cataliotti, A., Tachibana, I., & Burnett, J. C. J. (2003). Ventricular cardiotrophin-1 activation precedes BNP in experimental heart failure. Peptides, 24(6), 889-892. https://doi.org/10.1016/S0196-9781(03)00163-3

Ventricular cardiotrophin-1 activation precedes BNP in experimental heart failure. / Jougasaki, Michihisa; Leskinen, Hanna; Larsen, Amy M.; Luchner, Andreas; Cataliotti, Alessandro; Tachibana, Issei; Burnett, John C Jr.

In: Peptides, Vol. 24, No. 6, 01.06.2003, p. 889-892.

Research output: Contribution to journalArticle

Jougasaki, M, Leskinen, H, Larsen, AM, Luchner, A, Cataliotti, A, Tachibana, I & Burnett, JCJ 2003, 'Ventricular cardiotrophin-1 activation precedes BNP in experimental heart failure', Peptides, vol. 24, no. 6, pp. 889-892. https://doi.org/10.1016/S0196-9781(03)00163-3
Jougasaki M, Leskinen H, Larsen AM, Luchner A, Cataliotti A, Tachibana I et al. Ventricular cardiotrophin-1 activation precedes BNP in experimental heart failure. Peptides. 2003 Jun 1;24(6):889-892. https://doi.org/10.1016/S0196-9781(03)00163-3
Jougasaki, Michihisa ; Leskinen, Hanna ; Larsen, Amy M. ; Luchner, Andreas ; Cataliotti, Alessandro ; Tachibana, Issei ; Burnett, John C Jr. / Ventricular cardiotrophin-1 activation precedes BNP in experimental heart failure. In: Peptides. 2003 ; Vol. 24, No. 6. pp. 889-892.
@article{98c132030d4c4110ae4ef7853e187463,
title = "Ventricular cardiotrophin-1 activation precedes BNP in experimental heart failure",
abstract = "Both cardiotrophin-1 (CT-1) and B-type or brain natriuretic peptide (BNP) are activated by cardiomyocyte stretch, and gene expression of CT-1 and BNP are augmented in the heart in experimental and human congestive heart failure (CHF). The goal of this study was to define cardiac gene expression of CT-1 and BNP by Northern blot analysis in normal (n=5), early left ventricular dysfunction (ELVD, n=5) and overt CHF dogs (n=5), in which ventricular function is progressively decreased. CT-1 mRNA was detected in both atria and ventricles in normal dogs. Ventricular CT-1 mRNA production increased in ELVD, and it further increased in overt CHF. Ventricular BNP mRNA remained below or at the limit of detection in normal and ELVD models, and it markedly increased in overt CHF. This study reports differential regulation of gene expression of CT-1 and BNP in the heart during the progression of CHF, and demonstrates that ventricular CT-1 gene activation precedes ventricular BNP gene activation.",
keywords = "BNP, Cardiotrophin-1, Congestive heart failure, Cytokines, Northern blot analysis",
author = "Michihisa Jougasaki and Hanna Leskinen and Larsen, {Amy M.} and Andreas Luchner and Alessandro Cataliotti and Issei Tachibana and Burnett, {John C Jr.}",
year = "2003",
month = "6",
day = "1",
doi = "10.1016/S0196-9781(03)00163-3",
language = "English (US)",
volume = "24",
pages = "889--892",
journal = "Peptides",
issn = "0196-9781",
publisher = "Elsevier Inc.",
number = "6",

}

TY - JOUR

T1 - Ventricular cardiotrophin-1 activation precedes BNP in experimental heart failure

AU - Jougasaki, Michihisa

AU - Leskinen, Hanna

AU - Larsen, Amy M.

AU - Luchner, Andreas

AU - Cataliotti, Alessandro

AU - Tachibana, Issei

AU - Burnett, John C Jr.

PY - 2003/6/1

Y1 - 2003/6/1

N2 - Both cardiotrophin-1 (CT-1) and B-type or brain natriuretic peptide (BNP) are activated by cardiomyocyte stretch, and gene expression of CT-1 and BNP are augmented in the heart in experimental and human congestive heart failure (CHF). The goal of this study was to define cardiac gene expression of CT-1 and BNP by Northern blot analysis in normal (n=5), early left ventricular dysfunction (ELVD, n=5) and overt CHF dogs (n=5), in which ventricular function is progressively decreased. CT-1 mRNA was detected in both atria and ventricles in normal dogs. Ventricular CT-1 mRNA production increased in ELVD, and it further increased in overt CHF. Ventricular BNP mRNA remained below or at the limit of detection in normal and ELVD models, and it markedly increased in overt CHF. This study reports differential regulation of gene expression of CT-1 and BNP in the heart during the progression of CHF, and demonstrates that ventricular CT-1 gene activation precedes ventricular BNP gene activation.

AB - Both cardiotrophin-1 (CT-1) and B-type or brain natriuretic peptide (BNP) are activated by cardiomyocyte stretch, and gene expression of CT-1 and BNP are augmented in the heart in experimental and human congestive heart failure (CHF). The goal of this study was to define cardiac gene expression of CT-1 and BNP by Northern blot analysis in normal (n=5), early left ventricular dysfunction (ELVD, n=5) and overt CHF dogs (n=5), in which ventricular function is progressively decreased. CT-1 mRNA was detected in both atria and ventricles in normal dogs. Ventricular CT-1 mRNA production increased in ELVD, and it further increased in overt CHF. Ventricular BNP mRNA remained below or at the limit of detection in normal and ELVD models, and it markedly increased in overt CHF. This study reports differential regulation of gene expression of CT-1 and BNP in the heart during the progression of CHF, and demonstrates that ventricular CT-1 gene activation precedes ventricular BNP gene activation.

KW - BNP

KW - Cardiotrophin-1

KW - Congestive heart failure

KW - Cytokines

KW - Northern blot analysis

UR - http://www.scopus.com/inward/record.url?scp=0042430588&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0042430588&partnerID=8YFLogxK

U2 - 10.1016/S0196-9781(03)00163-3

DO - 10.1016/S0196-9781(03)00163-3

M3 - Article

VL - 24

SP - 889

EP - 892

JO - Peptides

JF - Peptides

SN - 0196-9781

IS - 6

ER -