Vasoactive intestinal polypeptide: A putative transmitter in the canine gastric muscularis mucosa

F. Angel, V. L W Go, P. F. Schmalz, J. H. Szurszewski

Research output: Contribution to journalArticle

65 Citations (Scopus)

Abstract

The nature of the inhibitory transmitter in the canine gastric muscularis mucosae was studied in vitro using superfusion techniques. The inhibitory effect of nerve stimulation (10 V, 200 μs, 10 Hz) was not altered by adrenergic, cholinergic or serotonergic antagonists. Adenosine triphosphate had no effect on spontaneous mechanical activity. Nucleotide pyrophosphatase and apamin had no effect on the response to nerve stimulation. α-Chymotrypsin abolished the inhibitory effect of nerve stimulation. Radioimmunoassay of the muscle indicated the presence of gastrin/cholecystokinin-substance P- and vasoactive intestinal polypeptide (VIP)-like immunoreactivity. Of the three peptides present, only VIP produced a concentration-dependent relaxation. A substance with VIP-like immunoreactivity was released during nerve-induced relaxation of the muscle, and its release was blocked by tetrodotoxin and calcium-depleted solution. The inhibitory effect of nerve stimulation was abolished by VIP antiserum. These data strongly support the hypothesis that VIP or a closely related peptide is an inhibitory neurotransmitter in the canine gastric muscularis mucosae.

Original languageEnglish (US)
Pages (from-to)641-654
Number of pages14
JournalJournal of Physiology
VolumeVol. 341
StatePublished - 1983
Externally publishedYes

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Vasoactive Intestinal Peptide
Gastric Mucosa
Canidae
Mucous Membrane
nucleotide pyrophosphatase
Apamin
Peptides
Muscle Relaxation
Cholecystokinin
Gastrins
Tetrodotoxin
Chymotrypsin
Substance P
Adrenergic Agents
Cholinergic Agents
Radioimmunoassay
Neurotransmitter Agents
Immune Sera
Adenosine Triphosphate
Calcium

ASJC Scopus subject areas

  • Physiology

Cite this

Angel, F., Go, V. L. W., Schmalz, P. F., & Szurszewski, J. H. (1983). Vasoactive intestinal polypeptide: A putative transmitter in the canine gastric muscularis mucosa. Journal of Physiology, Vol. 341, 641-654.

Vasoactive intestinal polypeptide : A putative transmitter in the canine gastric muscularis mucosa. / Angel, F.; Go, V. L W; Schmalz, P. F.; Szurszewski, J. H.

In: Journal of Physiology, Vol. Vol. 341, 1983, p. 641-654.

Research output: Contribution to journalArticle

Angel, F, Go, VLW, Schmalz, PF & Szurszewski, JH 1983, 'Vasoactive intestinal polypeptide: A putative transmitter in the canine gastric muscularis mucosa', Journal of Physiology, vol. Vol. 341, pp. 641-654.
Angel, F. ; Go, V. L W ; Schmalz, P. F. ; Szurszewski, J. H. / Vasoactive intestinal polypeptide : A putative transmitter in the canine gastric muscularis mucosa. In: Journal of Physiology. 1983 ; Vol. Vol. 341. pp. 641-654.
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