Vasoactive intestinal polypeptide: a putative transmitter in the canine gastric muscularis mucosa.

F. Angel, V. L. Go, P. F. Schmalz, J. H. Szurszewski

Research output: Contribution to journalArticle

65 Scopus citations

Abstract

The nature of the inhibitory transmitter in the canine gastric muscularis mucosae was studied in vitro using superfusion techniques. The inhibitory effect of nerve stimulation (10 V, 200 mus, 10 Hz) was not altered by adrenergic, cholinergic or serotonergic antagonists. Adenosine triphosphate had no effect on spontaneous mechanical activity. Nucleotide pyrophosphatase and apamin had no effect on the response to nerve stimulation. Alpha‐chymotrypsin abolished the inhibitory effect of nerve stimulation. Radioimmunoassay of the muscle indicated the presence of gastrin/cholecystokinin‐substance P‐ and vasoactive intestinal polypeptide (VIP)‐like immunoreactivity. Of the three peptides present, only VIP produced a concentration‐dependent relaxation. A substance with VIP‐like immunoreactivity was released during nerve‐induced relaxation of the muscle, and its release was blocked by tetrodotoxin and calcium‐depleted solution. The inhibitory effect of nerve stimulation was abolished by VIP antiserum. These data strongly support the hypothesis that VIP or a closely related peptide is an inhibitory neurotransmitter in the canine gastric muscularis mucosae.

Original languageEnglish (US)
Pages (from-to)641-654
Number of pages14
JournalThe Journal of Physiology
Volume341
Issue number1
DOIs
StatePublished - Aug 1 1983

ASJC Scopus subject areas

  • Physiology

Fingerprint Dive into the research topics of 'Vasoactive intestinal polypeptide: a putative transmitter in the canine gastric muscularis mucosa.'. Together they form a unique fingerprint.

  • Cite this