Vascularized bone grafting in a canine carpal avascular necrosis model

Wouter F. Willems, Gregory M. Alberton, Allen Thorp Bishop, Thomas Kremer

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Background: Limited experimental research has been performed on the treatment of avascular necrosis (AVN) by vascularized bone grafting. Questions/purposes: A new model simulating carpal AVN was created to investigate surgical revascularization of necrotic bone. Methods: In seven mongrel dogs, AVN was induced by removal of the radial carpal bones bilaterally, deep-freezing, coating in cyanoacrylate, and reimplantation. A reverse-flow vascularized bone graft from the distal radius was implanted in the avascular radial carpal bone. The contralateral side served as an untreated ischemic control. Bone blood flow, bone volume, radiography, histomorphometry, histology, and MRI were analyzed at 4 weeks. Results: Blood flow was substantially higher in grafted bones when compared with controls (14.68 ± 15.43 versus 0.27 ± 0.28 mL/minute/100 g). Blood flow correlated with increased osteoid formation and higher levels of bone turnover. T1 and T2 signals on MRI did not correlate with quantitative bone blood flow measurements. Necrotic bones with no blood flow had normal T1 and T2 signals, whereas revascularized bones had signal changes when compared with adjacent carpal bones. No major collapse occurred in any radiocarpal bone. Conclusion: In a canine experimental model, investigation of carpal AVN shows the ability of vascularized bone grafting to revascularize and remodel avascular bone. Clinical Relevance: Surgical revascularization of necrotic bone induced by vascularized bone grafting results in increased bone perfusion and bone remodeling as compared with untreated necrotic bone. MRI T1 and T2 signals can be normal in necrotic avascular bone.

Original languageEnglish (US)
Pages (from-to)2831-2837
Number of pages7
JournalClinical Orthopaedics and Related Research
Volume469
Issue number10
DOIs
StatePublished - Oct 2011

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Bone Transplantation
Wrist
Canidae
Necrosis
Bone and Bones
Carpal Bones
Bone Remodeling
Cyanoacrylates
Osteonecrosis
Replantation
Radiography
Freezing
Histology

ASJC Scopus subject areas

  • Orthopedics and Sports Medicine

Cite this

Vascularized bone grafting in a canine carpal avascular necrosis model. / Willems, Wouter F.; Alberton, Gregory M.; Bishop, Allen Thorp; Kremer, Thomas.

In: Clinical Orthopaedics and Related Research, Vol. 469, No. 10, 10.2011, p. 2831-2837.

Research output: Contribution to journalArticle

Willems, Wouter F. ; Alberton, Gregory M. ; Bishop, Allen Thorp ; Kremer, Thomas. / Vascularized bone grafting in a canine carpal avascular necrosis model. In: Clinical Orthopaedics and Related Research. 2011 ; Vol. 469, No. 10. pp. 2831-2837.
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