Vascular Injury in the Zebrafish Tail Modulates Blood Flow and Peak Wall Shear Stress to Restore Embryonic Circular Network

Kyung In Baek; Shyr Shea Chang; Chih Chiang Chang; Mehrdad Roustaei; Yichen Ding; Yixuan Wang; Justin Chen; Ryan O'Donnell; Hong Chen; Julianne W. Ashby; Xiaolei Xu; Julia J. Mack; Susana Cavallero; Marcus Roper; Tzung K. Hsiai

doi:10.3389/fcvm.2022.841101

Vascular Injury in the Zebrafish Tail Modulates Blood Flow and Peak Wall Shear Stress to Restore Embryonic Circular Network

Kyung In Baek, Shyr Shea Chang, Chih Chiang Chang, Mehrdad Roustaei, Yichen Ding, Yixuan Wang, Justin Chen, Ryan O'Donnell, Hong Chen, Julianne W. Ashby, Xiaolei Xu, Julia J. Mack, Susana Cavallero, Marcus Roper, Tzung K. Hsiai

Biochemistry and Molecular Biology

Research output: Contribution to journal › Article › peer-review

Abstract

Mechano-responsive signaling pathways enable blood vessels within a connected network to structurally adapt to partition of blood flow between organ systems. Wall shear stress (WSS) modulates endothelial cell proliferation and arteriovenous specification. Here, we study vascular regeneration in a zebrafish model by using tail amputation to disrupt the embryonic circulatory loop (ECL) at 3 days post fertilization (dpf). We observed a local increase in blood flow and peak WSS in the Segmental Artery (SeA) immediately adjacent to the amputation site. By manipulating blood flow and WSS via changes in blood viscosity and myocardial contractility, we show that the angiogenic Notch-ephrinb2 cascade is hemodynamically activated in the SeA to guide arteriogenesis and network reconnection. Taken together, ECL amputation induces changes in microvascular topology to partition blood flow and increase WSS-mediated Notch-ephrinb2 pathway, promoting new vascular arterial loop formation and restoring microcirculation.

Original language	English (US)
Article number	841101
Journal	Frontiers in Cardiovascular Medicine
Volume	9
DOIs	https://doi.org/10.3389/fcvm.2022.841101
State	Published - Mar 18 2022

Keywords

Notch-ephrinb2 signaling
biophysics
peak wall shear stress
vascular injury and repair
vascular loop formation

ASJC Scopus subject areas

Cardiology and Cardiovascular Medicine

Access to Document

10.3389/fcvm.2022.841101

Cite this

Baek, K. I., Chang, S. S., Chang, C. C., Roustaei, M., Ding, Y., Wang, Y., Chen, J., O'Donnell, R., Chen, H., Ashby, J. W., Xu, X., Mack, J. J., Cavallero, S., Roper, M., & Hsiai, T. K. (2022). Vascular Injury in the Zebrafish Tail Modulates Blood Flow and Peak Wall Shear Stress to Restore Embryonic Circular Network. Frontiers in Cardiovascular Medicine, 9, Article 841101. https://doi.org/10.3389/fcvm.2022.841101

Baek, KI, Chang, SS, Chang, CC, Roustaei, M, Ding, Y, Wang, Y, Chen, J, O'Donnell, R, Chen, H, Ashby, JW, Xu, X, Mack, JJ, Cavallero, S, Roper, M & Hsiai, TK 2022, 'Vascular Injury in the Zebrafish Tail Modulates Blood Flow and Peak Wall Shear Stress to Restore Embryonic Circular Network', Frontiers in Cardiovascular Medicine, vol. 9, 841101. https://doi.org/10.3389/fcvm.2022.841101

@article{f86be4ca70be4734a383557f6c0fb1e2,

title = "Vascular Injury in the Zebrafish Tail Modulates Blood Flow and Peak Wall Shear Stress to Restore Embryonic Circular Network",

abstract = "Mechano-responsive signaling pathways enable blood vessels within a connected network to structurally adapt to partition of blood flow between organ systems. Wall shear stress (WSS) modulates endothelial cell proliferation and arteriovenous specification. Here, we study vascular regeneration in a zebrafish model by using tail amputation to disrupt the embryonic circulatory loop (ECL) at 3 days post fertilization (dpf). We observed a local increase in blood flow and peak WSS in the Segmental Artery (SeA) immediately adjacent to the amputation site. By manipulating blood flow and WSS via changes in blood viscosity and myocardial contractility, we show that the angiogenic Notch-ephrinb2 cascade is hemodynamically activated in the SeA to guide arteriogenesis and network reconnection. Taken together, ECL amputation induces changes in microvascular topology to partition blood flow and increase WSS-mediated Notch-ephrinb2 pathway, promoting new vascular arterial loop formation and restoring microcirculation.",

keywords = "Notch-ephrinb2 signaling, biophysics, peak wall shear stress, vascular injury and repair, vascular loop formation",

author = "Baek, {Kyung In} and Chang, {Shyr Shea} and Chang, {Chih Chiang} and Mehrdad Roustaei and Yichen Ding and Yixuan Wang and Justin Chen and Ryan O'Donnell and Hong Chen and Ashby, {Julianne W.} and Xiaolei Xu and Mack, {Julia J.} and Susana Cavallero and Marcus Roper and Hsiai, {Tzung K.}",

note = "Publisher Copyright: Copyright {\textcopyright} 2022 Baek, Chang, Chang, Roustaei, Ding, Wang, Chen, O'Donnell, Chen, Ashby, Xu, Mack, Cavallero, Roper and Hsiai.",

year = "2022",

month = mar,

day = "18",

doi = "10.3389/fcvm.2022.841101",

language = "English (US)",

volume = "9",

journal = "Frontiers in Cardiovascular Medicine",

issn = "2297-055X",

publisher = "Frontiers Media S. A.",

}

TY - JOUR

T1 - Vascular Injury in the Zebrafish Tail Modulates Blood Flow and Peak Wall Shear Stress to Restore Embryonic Circular Network

AU - Baek, Kyung In

AU - Chang, Shyr Shea

AU - Chang, Chih Chiang

AU - Roustaei, Mehrdad

AU - Ding, Yichen

AU - Wang, Yixuan

AU - Chen, Justin

AU - O'Donnell, Ryan

AU - Chen, Hong

AU - Ashby, Julianne W.

AU - Xu, Xiaolei

AU - Mack, Julia J.

AU - Cavallero, Susana

AU - Roper, Marcus

AU - Hsiai, Tzung K.

PY - 2022/3/18

Y1 - 2022/3/18

N2 - Mechano-responsive signaling pathways enable blood vessels within a connected network to structurally adapt to partition of blood flow between organ systems. Wall shear stress (WSS) modulates endothelial cell proliferation and arteriovenous specification. Here, we study vascular regeneration in a zebrafish model by using tail amputation to disrupt the embryonic circulatory loop (ECL) at 3 days post fertilization (dpf). We observed a local increase in blood flow and peak WSS in the Segmental Artery (SeA) immediately adjacent to the amputation site. By manipulating blood flow and WSS via changes in blood viscosity and myocardial contractility, we show that the angiogenic Notch-ephrinb2 cascade is hemodynamically activated in the SeA to guide arteriogenesis and network reconnection. Taken together, ECL amputation induces changes in microvascular topology to partition blood flow and increase WSS-mediated Notch-ephrinb2 pathway, promoting new vascular arterial loop formation and restoring microcirculation.

AB - Mechano-responsive signaling pathways enable blood vessels within a connected network to structurally adapt to partition of blood flow between organ systems. Wall shear stress (WSS) modulates endothelial cell proliferation and arteriovenous specification. Here, we study vascular regeneration in a zebrafish model by using tail amputation to disrupt the embryonic circulatory loop (ECL) at 3 days post fertilization (dpf). We observed a local increase in blood flow and peak WSS in the Segmental Artery (SeA) immediately adjacent to the amputation site. By manipulating blood flow and WSS via changes in blood viscosity and myocardial contractility, we show that the angiogenic Notch-ephrinb2 cascade is hemodynamically activated in the SeA to guide arteriogenesis and network reconnection. Taken together, ECL amputation induces changes in microvascular topology to partition blood flow and increase WSS-mediated Notch-ephrinb2 pathway, promoting new vascular arterial loop formation and restoring microcirculation.

KW - Notch-ephrinb2 signaling

KW - biophysics

KW - peak wall shear stress

KW - vascular injury and repair

KW - vascular loop formation

UR - http://www.scopus.com/inward/record.url?scp=85138523888&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85138523888&partnerID=8YFLogxK

U2 - 10.3389/fcvm.2022.841101

DO - 10.3389/fcvm.2022.841101

M3 - Article

AN - SCOPUS:85138523888

SN - 2297-055X

VL - 9

JO - Frontiers in Cardiovascular Medicine

JF - Frontiers in Cardiovascular Medicine

M1 - 841101

ER -

Vascular Injury in the Zebrafish Tail Modulates Blood Flow and Peak Wall Shear Stress to Restore Embryonic Circular Network

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this