Variable expression of IL-1β has minimal effect on the radiation sensitivity of three human glioma cell lines

H. J. Ross, R. J. Antoniono, J. A. Buckmeier, J. L. Redpath

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

Interleukin 1 (IL-1) has been reported to act as a radioprotector in vivo. Data from our laboratory and from other investigators suggest that glioma cell lines can produce bioactive cytokines including IL-1 and also express IL-1 receptors. In view of the putative radioprotective effect of this cytokine, we have examined the in vitro radiosensitivity of three human glioma cell lines with widely varying levels of endogenous IL-1β. The data reveal that when irradiated (2 Gy/min) as confluent cultures (conditions optimal for differential IL-1β expression), and plated for colony formation after postirradiation holding, cell survival was not correlated with level of IL-1β mRNA expression. Retinoic acid has been shown to dramatically enhance levels of IL-1β mRNA expression in the two IL-1-expressing cell lines. However, this was not correlated with a further reduction in radiosensitivity. These data indicate that IL-1β does not act as an endogenous radioprotector in these cells under these experimental conditions.

Original languageEnglish (US)
Pages (from-to)785-791
Number of pages7
JournalInternational Journal of Radiation Biology
Volume66
Issue number6
StatePublished - 1994

ASJC Scopus subject areas

  • Radiological and Ultrasound Technology
  • Radiology Nuclear Medicine and imaging

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