Validation of the measurement of low concentrations of 5-hydroxytryptamine in plasma using high performance liquid chromatography

Wendy Atkinson, Stephen J. Lockhart, Lesley A. Houghton, Brian G. Keevil

Research output: Contribution to journalArticle

14 Scopus citations

Abstract

A sensitive and rapid assay is described for the measurement of low concentrations of 5-hydroxytryptamine (5-HT) present in human platelet-depleted plasma (PDP) using reverse-phase high performance liquid chromatography (HPLC) with fluorimetric detection. With an analysis time of 12 min, this method is particularly useful for large-scale clinical trials investigating small differences in PDP 5-HT concentrations in conditions such as functional gastrointestinal disorders (FGID). The limit of detection and quantification were 1 and 3 nmol/l, respectively, and the calibration curve linear between 1 and 1000 nmol/l. The within-day and between-day precision were 4.3 and <13.6%, respectively.

Original languageEnglish (US)
Pages (from-to)173-176
Number of pages4
JournalJournal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume832
Issue number1
DOIs
StatePublished - Feb 17 2006

Keywords

  • 5-HT
  • HPLC
  • Plasma
  • Validation

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry
  • Cell Biology

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