Validation of the measurement of low concentrations of 5-hydroxytryptamine in plasma using high performance liquid chromatography

Wendy Atkinson, Stephen J. Lockhart, Lesley A. Houghton, Brian G. Keevil

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

A sensitive and rapid assay is described for the measurement of low concentrations of 5-hydroxytryptamine (5-HT) present in human platelet-depleted plasma (PDP) using reverse-phase high performance liquid chromatography (HPLC) with fluorimetric detection. With an analysis time of 12 min, this method is particularly useful for large-scale clinical trials investigating small differences in PDP 5-HT concentrations in conditions such as functional gastrointestinal disorders (FGID). The limit of detection and quantification were 1 and 3 nmol/l, respectively, and the calibration curve linear between 1 and 1000 nmol/l. The within-day and between-day precision were 4.3 and <13.6%, respectively.

Original languageEnglish (US)
Pages (from-to)173-176
Number of pages4
JournalJournal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume832
Issue number1
DOIs
StatePublished - Feb 17 2006
Externally publishedYes

Fingerprint

High performance liquid chromatography
Platelets
Serotonin
Blood Platelets
High Pressure Liquid Chromatography
Plasmas
Gastrointestinal Diseases
Reverse-Phase Chromatography
Calibration
Limit of Detection
Assays
Clinical Trials

Keywords

  • 5-HT
  • HPLC
  • Plasma
  • Validation

ASJC Scopus subject areas

  • Biochemistry

Cite this

Validation of the measurement of low concentrations of 5-hydroxytryptamine in plasma using high performance liquid chromatography. / Atkinson, Wendy; Lockhart, Stephen J.; Houghton, Lesley A.; Keevil, Brian G.

In: Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, Vol. 832, No. 1, 17.02.2006, p. 173-176.

Research output: Contribution to journalArticle

@article{ec7000d81dea4101b2630f6dfa36a71f,
title = "Validation of the measurement of low concentrations of 5-hydroxytryptamine in plasma using high performance liquid chromatography",
abstract = "A sensitive and rapid assay is described for the measurement of low concentrations of 5-hydroxytryptamine (5-HT) present in human platelet-depleted plasma (PDP) using reverse-phase high performance liquid chromatography (HPLC) with fluorimetric detection. With an analysis time of 12 min, this method is particularly useful for large-scale clinical trials investigating small differences in PDP 5-HT concentrations in conditions such as functional gastrointestinal disorders (FGID). The limit of detection and quantification were 1 and 3 nmol/l, respectively, and the calibration curve linear between 1 and 1000 nmol/l. The within-day and between-day precision were 4.3 and <13.6{\%}, respectively.",
keywords = "5-HT, HPLC, Plasma, Validation",
author = "Wendy Atkinson and Lockhart, {Stephen J.} and Houghton, {Lesley A.} and Keevil, {Brian G.}",
year = "2006",
month = "2",
day = "17",
doi = "10.1016/j.jchromb.2005.12.038",
language = "English (US)",
volume = "832",
pages = "173--176",
journal = "Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences",
issn = "1570-0232",
publisher = "Elsevier",
number = "1",

}

TY - JOUR

T1 - Validation of the measurement of low concentrations of 5-hydroxytryptamine in plasma using high performance liquid chromatography

AU - Atkinson, Wendy

AU - Lockhart, Stephen J.

AU - Houghton, Lesley A.

AU - Keevil, Brian G.

PY - 2006/2/17

Y1 - 2006/2/17

N2 - A sensitive and rapid assay is described for the measurement of low concentrations of 5-hydroxytryptamine (5-HT) present in human platelet-depleted plasma (PDP) using reverse-phase high performance liquid chromatography (HPLC) with fluorimetric detection. With an analysis time of 12 min, this method is particularly useful for large-scale clinical trials investigating small differences in PDP 5-HT concentrations in conditions such as functional gastrointestinal disorders (FGID). The limit of detection and quantification were 1 and 3 nmol/l, respectively, and the calibration curve linear between 1 and 1000 nmol/l. The within-day and between-day precision were 4.3 and <13.6%, respectively.

AB - A sensitive and rapid assay is described for the measurement of low concentrations of 5-hydroxytryptamine (5-HT) present in human platelet-depleted plasma (PDP) using reverse-phase high performance liquid chromatography (HPLC) with fluorimetric detection. With an analysis time of 12 min, this method is particularly useful for large-scale clinical trials investigating small differences in PDP 5-HT concentrations in conditions such as functional gastrointestinal disorders (FGID). The limit of detection and quantification were 1 and 3 nmol/l, respectively, and the calibration curve linear between 1 and 1000 nmol/l. The within-day and between-day precision were 4.3 and <13.6%, respectively.

KW - 5-HT

KW - HPLC

KW - Plasma

KW - Validation

UR - http://www.scopus.com/inward/record.url?scp=32244433356&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=32244433356&partnerID=8YFLogxK

U2 - 10.1016/j.jchromb.2005.12.038

DO - 10.1016/j.jchromb.2005.12.038

M3 - Article

C2 - 16442351

AN - SCOPUS:32244433356

VL - 832

SP - 173

EP - 176

JO - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences

JF - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences

SN - 1570-0232

IS - 1

ER -