Validation of Osteogenic Properties of Cytochalasin D by High-Resolution RNA-Sequencing in Mesenchymal Stem Cells Derived from Bone Marrow and Adipose Tissues

Rebekah M. Samsonraj, Christopher R. Paradise, Amel Dudakovic, Buer Sen, Asha A. Nair, Allan B Dietz, David R Deyle, Simon M. Cool, Janet Rubin, Andre J van Wijnen

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Differentiation of mesenchymal stromal/stem cells (MSCs) involves a series of molecular signals and gene transcription events required for attaining cell lineage commitment. Modulation of the actin cytoskeleton using cytochalasin D (CytoD) drives osteogenesis at early timepoints in bone marrow-derived MSCs and also initiates a robust osteogenic differentiation program in adipose tissue-derived MSCs. To understand the molecular basis for these pronounced effects on osteogenic differentiation, we investigated global changes in gene expression in CytoD-treated murine and human MSCs by high-resolution RNA-sequencing (RNA-seq) analysis. A three-way bioinformatic comparison between human adipose tissue-derived MSCs (hAMSCs), human bone marrow-derived MSCs (hBMSCs), and mouse bone marrow-derived MSCs (mBMSCs) revealed significant upregulation of genes linked to extracellular matrix organization, cell adhesion and bone metabolism. As anticipated, the activation of these differentiation-related genes is accompanied by a downregulation of nuclear and cell cycle-related genes presumably reflecting cytostatic effects of CytoD. We also identified eight novel CytoD activated genes - VGLL4, ARHGAP24, KLHL24, RCBTB2, BDH2, SCARF2, ACAD10, HEPH - which are commonly upregulated across the two species and tissue sources of our MSC samples. We selected the Hippo pathway-related VGLL4 gene, which encodes the transcriptional co-factor Vestigial-like 4, for further study because this pathway is linked to osteogenesis. VGLL4 small interfering RNA depletion reduces mineralization of hAMSCs during CytoD-induced osteogenic differentiation. Together, our RNA-seq analyses suggest that while the stimulatory effects of CytoD on osteogenesis are pleiotropic and depend on the biological state of the cell type, a small group of genes including VGLL4 may contribute to MSC commitment toward the bone lineage.

Original languageEnglish (US)
Pages (from-to)1136-1145
Number of pages10
JournalStem Cells and Development
Volume27
Issue number16
DOIs
StatePublished - Aug 15 2018

Fingerprint

RNA Sequence Analysis
Cytochalasin D
Mesenchymal Stromal Cells
Adipose Tissue
Bone Marrow
Bone and Bones
Osteogenesis
Genes
RNA
cdc Genes
Cytostatic Agents

Keywords

  • bone
  • cell signaling
  • cytochalasin D
  • mesenchymal stromal cell
  • osteoblast
  • osteogenesis
  • stem cell

ASJC Scopus subject areas

  • Hematology
  • Developmental Biology
  • Cell Biology

Cite this

Validation of Osteogenic Properties of Cytochalasin D by High-Resolution RNA-Sequencing in Mesenchymal Stem Cells Derived from Bone Marrow and Adipose Tissues. / Samsonraj, Rebekah M.; Paradise, Christopher R.; Dudakovic, Amel; Sen, Buer; Nair, Asha A.; Dietz, Allan B; Deyle, David R; Cool, Simon M.; Rubin, Janet; van Wijnen, Andre J.

In: Stem Cells and Development, Vol. 27, No. 16, 15.08.2018, p. 1136-1145.

Research output: Contribution to journalArticle

Samsonraj, Rebekah M. ; Paradise, Christopher R. ; Dudakovic, Amel ; Sen, Buer ; Nair, Asha A. ; Dietz, Allan B ; Deyle, David R ; Cool, Simon M. ; Rubin, Janet ; van Wijnen, Andre J. / Validation of Osteogenic Properties of Cytochalasin D by High-Resolution RNA-Sequencing in Mesenchymal Stem Cells Derived from Bone Marrow and Adipose Tissues. In: Stem Cells and Development. 2018 ; Vol. 27, No. 16. pp. 1136-1145.
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