Validation of deconvolution analysis of LH secretion and half-life

T. Mulligan, H. A. Delemarre-van de Waal, M. L. Johnson, J. D. Veldhuis

Research output: Contribution to journalArticlepeer-review

33 Scopus citations

Abstract

Deconvolution methods constitute a class of analytic tools to quantitate hormone secretion and/or clearance in vivo. Although mathematically rigorous, deconvolution techniques have assumed, rather than proven, validity. Accordingly, we tested the validity of deconvolution analysis on true- positive human, animal (sheep and monkey), and computer-simulated data using the luteinizing hormone (LH) pulse signal as a relevant paradigm. We found that multiparameter deconvolution analysis has high discriminative sensitivity (human data 91%, animal 81%, computer-simulated 95%) and specificity (human 90%, animal 81%, computer-simulated 100%). Sensitivity was impaired by low secretory burst amplitude (< 0.1 IU · l-1 · min-1), short interpulse interval (< 60 min), infrequent venous sampling (every 20- 30 min), and high random experimental variation (e.g., noise > 15%). Specificity was hindered by noise. Deconvolution accurately characterized the unknown hormone half-life (r = +0.994) and production rate (r = +0.990). Interoperator reliability was high when statistically based criteria for secretory pulse detection were applied. We conclude that multiparameter deconvolution, within recognizable constraints, is a valid and reliable tool for in vivo investigation of hormone secretion and half-life.

Original languageEnglish (US)
Pages (from-to)R202-R211
JournalAmerican Journal of Physiology - Regulatory Integrative and Comparative Physiology
Volume267
Issue number1 36-1
DOIs
StatePublished - 1994

Keywords

  • hormone secretion
  • pulse analysis

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)

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