Validation of cell-based fluorescence assays: Practice guidelines from the ICSH and ICCS - Part v - performance criteria

Brent Wood, Dragan Jevremovic, Marie C. Béné, Ming Yan, Patrick Jacobs, Virginia Litwin

Research output: Contribution to journalReview article

74 Scopus citations


Multi-color flow cytometry is a unique technology, which enables the analysis of heterogeneous cellular systems and provides multiparametric information on a cell-by-cell basis. A variety of factors contribute to the complexity of validating cell-based flow cytometric methods, including the lack of fully characterized cellular reference materials and the difficulty in obtaining, or creating, samples with varying levels of a given cell type or varying levels of expression of a given antigen. This document summarizes validation requirements and describes validation strategies for quasi-quantitative and qualitative cell-based flow cytometric assays.

Original languageEnglish (US)
Pages (from-to)315-323
Number of pages9
JournalCytometry Part B - Clinical Cytometry
Issue number5
StatePublished - Sep 1 2013



  • Cytometry
  • accuracy
  • imprecision
  • sensitivity
  • specificity

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Histology
  • Cell Biology

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