Validation of cell-based fluorescence assays: Practice guidelines from the ICSH and ICCS - Part v - performance criteria

Brent Wood, Dragan Jevremovic, Marie C. Béné, Ming Yan, Patrick Jacobs, Virginia Litwin

Research output: Contribution to journalReview article

72 Citations (Scopus)

Abstract

Multi-color flow cytometry is a unique technology, which enables the analysis of heterogeneous cellular systems and provides multiparametric information on a cell-by-cell basis. A variety of factors contribute to the complexity of validating cell-based flow cytometric methods, including the lack of fully characterized cellular reference materials and the difficulty in obtaining, or creating, samples with varying levels of a given cell type or varying levels of expression of a given antigen. This document summarizes validation requirements and describes validation strategies for quasi-quantitative and qualitative cell-based flow cytometric assays.

Original languageEnglish (US)
Pages (from-to)315-323
Number of pages9
JournalCytometry Part B - Clinical Cytometry
Volume84
Issue number5
DOIs
StatePublished - Sep 1 2013

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Practice Guidelines
Fluorescence
Flow Cytometry
Color
Technology
Antigens

Keywords

  • accuracy
  • Cytometry
  • imprecision
  • sensitivity
  • specificity

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Histology
  • Cell Biology

Cite this

Validation of cell-based fluorescence assays : Practice guidelines from the ICSH and ICCS - Part v - performance criteria. / Wood, Brent; Jevremovic, Dragan; Béné, Marie C.; Yan, Ming; Jacobs, Patrick; Litwin, Virginia.

In: Cytometry Part B - Clinical Cytometry, Vol. 84, No. 5, 01.09.2013, p. 315-323.

Research output: Contribution to journalReview article

Wood, Brent ; Jevremovic, Dragan ; Béné, Marie C. ; Yan, Ming ; Jacobs, Patrick ; Litwin, Virginia. / Validation of cell-based fluorescence assays : Practice guidelines from the ICSH and ICCS - Part v - performance criteria. In: Cytometry Part B - Clinical Cytometry. 2013 ; Vol. 84, No. 5. pp. 315-323.
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